In this paper, one-dimensional Hamiltonian operators with spectral parameter-dependent boundary conditions are investigated. First, the eigenvalues of the problem under consideration are transformed into the eigenvalues of an operator in an appropriate Hilbert space. Then, some properties of the eigenvalues are given. Moreover, the continuity and differentiability of the eigenvalues of the problem are obtained, and the differential expressions of the eigenvalues concerning each parameter are also given. Finally, Green’s function is also involved.

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