In this paper, we provide a detailed analysis of the analytic continuation of the spectral zeta function associated with one-dimensional regular Sturm-Liouville problems endowed with self-adjoint separated and coupled boundary conditions. The spectral zeta function is represented in terms of a complex integral and the analytic continuation in the entire complex plane is achieved by using the well-known Liouville-Green (or WKB) asymptotic expansion of the eigenfunctions associated with the problem. The analytically continued expression of the spectral zeta function is then used to compute the functional determinant of the Sturm-Liouville operator and the coefficients of the asymptotic expansion of the associated heat kernel.
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