The intriguing network of antibody–antigen (Ab–Ag) interactions is highly governed by environmental perturbations and the nature of biomolecular interaction. Protein–protein interactions (PPIs) have potential applications in developing protein-adsorption-based sensors and nano-scale materials. Therefore, characterizing PPIs in the presence of a nanomaterial at the molecular level becomes imperative. The present work involves the investigation of antiferritin–ferritin (Ab–Ag) protein interactions under the influence of tungsten disulfide quantum dots (WS2 QDs). Isothermal calorimetry and contact angle measurements validated the strong influence of WS2 QDs on Ab–Ag interactions. The interfacial signatures of nano–bio-interactions were evaluated using sum frequency generation vibration spectroscopy (SFG-VS) at the air–water interface. Our SFG results reveal a variation in the tilt angle of methyl groups by ∼12° ± 2° for the Ab–Ag system in the presence of WS2 QDs. The results illustrated an enhanced ordering of water molecules in the presence of QDs, which underpins the active role of interfacial water molecules during nano–bio-interactions. We have also witnessed a differential impact of QDs on Ab–Ag by raising the concentration of the Ab–Ag combination, which showcased an increased inter-molecular interaction among the Ab and Ag molecules and a minimal influence on the methyl tilt angle. These findings suggest the formation of stronger and ordered Ab–Ag complexes upon introducing WS2 QDs in the aqueous medium and signify the potentiality of WS2 QDs relevant to protein-based sensing assays.
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28 February 2024
Research Article|
February 27 2024
Role of tungsten disulfide quantum dots in specific protein–protein interactions at air–water interface
Special Collection:
Recent Developments in Nonlinear Optics at Interfaces
Harsharan Kaur
;
Harsharan Kaur
(Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing)
1
Department of Biomedical Engineering, Indian Institute of Technology Ropar
, Rupnagar, Punjab 140001, India
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Mayank Garg
;
Mayank Garg
(Conceptualization, Data curation, Investigation, Methodology, Writing – original draft, Writing – review & editing)
2
CSIR-Central Scientific Instruments Organisation (CSIR-CSIO)
, Chandigarh 160030, India
3
Academy of Scientific and Innovative Research (AcSIR)
, Ghaziabad 201002, India
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Deepak Tomar
;
Deepak Tomar
(Conceptualization, Formal analysis, Writing – review & editing)
4
Department of Physics, Indian Institute of Technology Ropar
, Rupnagar, Punjab 140001, India
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Suman Singh
;
Suman Singh
a)
(Conceptualization, Formal analysis, Funding acquisition, Supervision, Writing – review & editing)
2
CSIR-Central Scientific Instruments Organisation (CSIR-CSIO)
, Chandigarh 160030, India
3
Academy of Scientific and Innovative Research (AcSIR)
, Ghaziabad 201002, India
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Kailash C. Jena
Kailash C. Jena
a)
(Conceptualization, Formal analysis, Funding acquisition, Supervision, Validation, Writing – review & editing)
1
Department of Biomedical Engineering, Indian Institute of Technology Ropar
, Rupnagar, Punjab 140001, India
4
Department of Physics, Indian Institute of Technology Ropar
, Rupnagar, Punjab 140001, India
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J. Chem. Phys. 160, 084705 (2024)
Article history
Received:
November 14 2023
Accepted:
February 05 2024
Citation
Harsharan Kaur, Mayank Garg, Deepak Tomar, Suman Singh, Kailash C. Jena; Role of tungsten disulfide quantum dots in specific protein–protein interactions at air–water interface. J. Chem. Phys. 28 February 2024; 160 (8): 084705. https://doi.org/10.1063/5.0187563
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