The intriguing network of antibody–antigen (Ab–Ag) interactions is highly governed by environmental perturbations and the nature of biomolecular interaction. Protein–protein interactions (PPIs) have potential applications in developing protein-adsorption-based sensors and nano-scale materials. Therefore, characterizing PPIs in the presence of a nanomaterial at the molecular level becomes imperative. The present work involves the investigation of antiferritin–ferritin (Ab–Ag) protein interactions under the influence of tungsten disulfide quantum dots (WS2 QDs). Isothermal calorimetry and contact angle measurements validated the strong influence of WS2 QDs on Ab–Ag interactions. The interfacial signatures of nano–bio-interactions were evaluated using sum frequency generation vibration spectroscopy (SFG-VS) at the air–water interface. Our SFG results reveal a variation in the tilt angle of methyl groups by ∼12° ± 2° for the Ab–Ag system in the presence of WS2 QDs. The results illustrated an enhanced ordering of water molecules in the presence of QDs, which underpins the active role of interfacial water molecules during nano–bio-interactions. We have also witnessed a differential impact of QDs on Ab–Ag by raising the concentration of the Ab–Ag combination, which showcased an increased inter-molecular interaction among the Ab and Ag molecules and a minimal influence on the methyl tilt angle. These findings suggest the formation of stronger and ordered Ab–Ag complexes upon introducing WS2 QDs in the aqueous medium and signify the potentiality of WS2 QDs relevant to protein-based sensing assays.

1.
K.
Fuxe
,
D. O.
Borroto-Escuela
,
W.
Romero-Fernandez
,
M.
Palkovits
,
A. O.
Tarakanov
,
F.
Ciruela
, and
L. F.
Agnati
,
Neuropsychopharmacol
39
,
131
(
2014
).
2.
I. S.
Moreira
,
P. A.
Fernandes
, and
M. J.
Ramos
,
Proteins: Struct., Funct., Bioinf.
68
,
803
(
2007
).
3.
J. H.
Morris
,
G. M.
Knudsen
,
E.
Verschueren
,
J. R.
Johnson
,
P.
Cimermancic
,
A. L.
Greninger
, and
A. R.
Pico
,
Nat. Protoc.
9
,
2539
(
2014
).
4.
J.
Qin
,
X.
Li
,
L.
Cao
,
S.
Du
,
W.
Wang
, and
S. Q.
Yao
,
J. Am. Chem. Soc.
142
,
417
(
2019
).
5.
M. G.
Plach
,
F.
Semmelmann
,
F.
Busch
,
M.
Busch
,
L.
Heizinger
,
V. H.
Wysocki
,
R.
Merkl
, and
R.
Sterner
,
Proc. Natl. Acad. Sci. U. S. A.
114
,
E8333
(
2017
).
6.
D. J.
Müller
and
Y. F.
Dufrene
,
Nat. Nanotechnol.
3
,
261
(
2008
).
7.
B.
Martial
,
T.
Lefevre
,
T.
Buffeteau
, and
M.
Auger
,
ACS Nano
13
,
3232
(
2019
).
8.
S.-H.
Park
,
W.
Ko
,
H. S.
Lee
, and
I.
Shin
,
J. Am. Chem. Soc.
141
,
4273
(
2019
).
9.
A. E.
Rydeen
,
E. M.
Brustad
, and
G. J.
Pielak
,
J. Am. Chem. Soc.
140
,
7441
(
2018
).
10.
N. C.
Yoder
and
K.
Kumar
,
J. Am. Chem. Soc.
128
,
188
(
2006
).
11.
A. M.
Watkins
,
R.
Bonneau
, and
P. S.
Arora
,
J. Am. Chem. Soc.
138
,
10386
(
2016
).
12.
R.
Mogaki
,
K.
Okuro
,
R.
Ueki
,
S.
Sando
, and
T.
Aida
,
J. Am. Chem. Soc.
141
,
8035
(
2019
).
13.
D.
Lis
and
F.
Cecchet
,
Chem. Phys. Chem.
17
,
2645
(
2016
).
14.
Y.
Hayamizu
,
C. R.
So
,
S.
Dag
,
T. S.
Page
,
D.
Starkebaum
, and
M.
Sarikaya
,
Sci. Rep.
6
,
33778
(
2016
).
15.
Q.
Ouyang
,
S.
Zeng
,
L.
Jiang
,
L.
Hong
,
G.
Xu
,
X.-Q.
Dinh
,
J.
Qian
,
S.
He
,
J.
Qu
, and
P.
Coquet
,
Sci. Rep.
6
,
28190
(
2016
).
16.
O.
Parlak
,
P.
Seshadri
,
I.
Lundström
,
A. P.
Turner
, and
A.
Tiwari
,
Adv. Mater. Interfaces
1
,
1400136
(
2014
).
17.
S.
Batool
,
M.
Idrees
,
M. S.
Javed
,
M.
Saleem
, and
J.
Kong
,
Mater. Chem. Phys.
246
,
122832
(
2020
).
18.
X.
Sun
,
J.
Fan
,
C.
Fu
,
L.
Yao
,
S.
Zhao
,
J.
Wang
, and
J.
Xiao
,
Sci. Rep.
7
,
10290
(
2017
).
19.
X.
Zhao
,
D.
He
,
Y.
Wang
, and
C.
Fu
,
Mater. Chem. Phys.
207
,
130
(
2018
).
20.
Y.
Yan
,
C.
Zhang
,
W.
Gu
,
C.
Ding
,
X.
Li
, and
Y.
Xian
,
J. Phys. Chem. C
120
,
12170
(
2016
).
21.
M.-J.
Kim
,
S.-J.
Jeon
,
T. W.
Kang
,
J.-M.
Ju
,
D.
Yim
,
H.-I.
Kim
,
J. H.
Park
, and
J.-H.
Kim
,
ACS Appl. Mater. Interfaces
9
,
12316
(
2017
).
22.
L.
Dreesen
,
Y.
Sartenaer
,
C.
Humbert
,
A. A.
Mani
,
J.-J.
Lemaire
,
C.
Méthivier
,
C.-M.
Pradier
,
P.
Thiry
, and
A.
Peremans
,
Thin Solid Films
464–465
,
373
(
2004
).
23.
B.
Saha
,
T. H.
Evers
, and
M. W.
Prins
,
Anal. Chem.
86
,
8158
(
2014
).
24.
M. L.
McDermott
,
H.
Vanselous
,
S. A.
Corcelli
, and
P. B.
Petersen
,
ACS Cent. Sci.
3
,
708
(
2017
).
25.
A. P.
Fellows
,
M. T.
Casford
, and
P. B.
Davies
,
AIP Adv.
11
,
045119
(
2021
).
26.
E. J.
Robertson
,
G. K.
Olivier
,
M.
Qian
,
C.
Proulx
,
R. N.
Zuckermann
, and
G. L.
Richmond
,
Proc. Natl. Acad. Sci. U. S. A.
111
,
13284
(
2014
).
27.
K.
Meister
,
S.
Strazdaite
,
A. L.
DeVries
,
S.
Lotze
,
L. L.
Olijve
,
I. K.
Voets
, and
H. J.
Bakker
,
Proc. Natl. Acad. Sci. U. S. A.
111
,
17732
(
2014
).
28.
S.
Chaudhary
,
H.
Kaur
,
H.
Kaur
,
B.
Rana
,
D.
Tomar
, and
K. C.
Jena
,
Appl. Spectrosc.
75
,
1497
(
2021
).
29.
K.
Engelhardt
,
U.
Weichsel
,
E.
Kraft
,
D.
Segets
,
W.
Peukert
, and
B.
Braunschweig
,
J. Phys. Chem. B
118
,
4098
(
2014
).
30.
J. A.
Mondal
,
S.
Nihonyanagi
,
S.
Yamaguchi
, and
T.
Tahara
,
J. Am. Chem. Soc.
134
,
7842
(
2012
).
31.
C. M.
Johnson
and
S.
Baldelli
,
Chem. Rev.
114
,
8416
(
2014
).
32.
S.
Chaudhary
,
H.
Kaur
,
H.
Kaur
, and
K. C.
Jena
,
Appl. Phys. Lett.
123
,
033703
(
2023
).
33.
A.
Ge
,
J. H.
Seo
,
L.
Qiao
,
N.
Yui
, and
S.
Ye
,
ACS Appl. Mater. Interfaces
7
,
22709
(
2015
).
34.
M.
Xiao
,
S.
Wei
,
Y.
Li
,
J.
Jasensky
,
J.
Chen
,
C. L.
Brooks
, and
Z.
Chen
,
Chem. Sci.
9
,
1769
(
2018
).
35.
M.
Xiao
,
S.
Wei
,
J.
Chen
,
J.
Tian
,
C. L.
Brooks
III
,
E. N. G.
Marsh
, and
Z.
Chen
,
J. Am. Chem. Soc.
141
,
9980
(
2019
).
36.
A. P.
Boughton
,
P.
Yang
,
V. M.
Tesmer
,
B.
Ding
,
J. J.
Tesmer
, and
Z.
Chen
,
Proc. Natl. Acad. Sci. U. S. A.
108
,
E667
(
2011
).
37.
M. A.
Knovich
,
J. A.
Storey
,
L. G.
Coffman
,
S. V.
Torti
, and
F. M.
Torti
,
Blood Rev.
23
,
95
(
2009
).
38.
G. n.
Jutz
,
P.
van Rijn
,
B.
Santos Miranda
, and
A.
Böker
,
Chem. Rev.
115
,
1653
(
2015
).
39.
S.
Allen
,
X.
Chen
,
J.
Davies
,
M. C.
Davies
,
A. C.
Dawkes
,
J. C.
Edwards
,
C. J.
Roberts
,
J.
Sefton
,
S. J.
Tendler
, and
P. M.
Williams
,
Biochemistry
36
,
7457
(
1997
).
40.
M.
Garg
,
M.
Chatterjee
,
A. L.
Sharma
, and
S.
Singh
,
Biosens. Bioelectron.
151
,
111979
(
2020
).
41.
H.
Kaur
,
D.
Tomar
,
H.
Kaur
,
B.
Rana
,
S.
Chaudhary
, and
K. C.
Jena
, in
Advances in Spectroscopy: Molecules to Materials: Proceedings of NCASMM 2018
, edited by
D. K.
Singh
,
S.
Das
and
A.
Materny
(
Springer Nature
,
2019
), Vol.
236
, p.
39
.
42.
H.
Kaur
,
S.
Chaudhary
,
H.
Kaur
,
M.
Chaudhary
, and
K. C.
Jena
,
ACS Appl. Nano Mater.
5
,
411
(
2021
).
43.
B.
Rana
,
D. J.
Fairhurst
, and
K. C.
Jena
,
J. Am. Chem. Soc.
144
,
17832
(
2022
).
44.
H.
Kaur
,
M.
Verma
,
S.
Kaur
,
B.
Rana
,
N.
Singh
, and
K. C.
Jena
,
Langmuir
38
,
13456
(
2022
).
45.
K. M.
Kazakbaeva
,
A.
Buglanov
, and
S.
Bakhramov
,
Chem. Nat. Compd.
22
,
90
(
1986
).
46.
E.
Gasteiger
,
C.
Hoogland
,
A.
Gattiker
,
M. R.
Wilkins
,
R. D.
Appel
, and
A.
Bairoch
,
The Proteomics Protocols Handbook
(
Springer
,
2005
), p.
571
.
47.
M. M.
Pierce
,
C.
Raman
, and
B. T.
Nall
,
Methods
19
,
213
(
1999
).
48.
E.
Omanovic-Miklicanin
,
I.
Manfield
, and
T.
Wilkins
,
J. Therm. Anal. Calorim.
127
,
605
(
2017
).
49.
P.
Mokaberi
,
F.
Babayan-Mashhadi
,
Z.
Amiri Tehrani Zadeh
,
M. R.
Saberi
, and
J.
Chamani
,
J. Biomol. Struct. Dyn.
39
,
3358
(
2021
).
51.
C.
Grote
,
K. J.
Chiad
,
D.
Vollmer
, and
G.
Garnweitner
,
Chem. Commun.
48
,
1464
(
2012
).
52.
Y.
Hong
,
H.
Zhou
,
W.
Qian
,
B.
Zuo
, and
X.
Wang
,
J. Phys. Chem. C
121
,
19816
(
2017
).
53.
E.
Tyrode
,
C. M.
Johnson
,
A.
Kumpulainen
,
M. W.
Rutland
, and
P. M.
Claesson
,
J. Am. Chem. Soc.
127
,
16848
(
2005
).
54.
J.
Kim
and
G. A.
Somorjai
,
J. Am. Chem. Soc.
125
,
3150
(
2003
).
55.
M.
Li
,
A.
Zhao
,
K.
Dong
,
W.
Li
,
J.
Ren
, and
X.
Qu
,
Nano Res.
8
,
3216
(
2015
).
56.
A. N.
Bordenyuk
,
C.
Weeraman
,
A.
Yatawara
,
H. D.
Jayathilake
,
I.
Stiopkin
,
Y.
Liu
, and
A. V.
Benderskii
,
J. Phys. Chem. C
111
,
8925
(
2007
).
57.
N.
Ji
and
Y.-R.
Shen
,
J. Chem. Phys.
120
,
7107
(
2004
).
58.
E. L.
Hommel
and
H. C.
Allen
,
Analyst
128
,
750
(
2003
).
59.
W.
Liu
,
L.
Fu
,
Z.
Wang
,
Z.
Sohrabpour
,
X.
Li
,
Y.
Liu
,
H. F.
Wang
, and
E. C. Y.
Yan
,
Phys. Chem. Chem. Phys.
20
,
22421
(
2018
).
60.
U. I.
Premadasa
,
N. M.
Adhikari
, and
K. L. A.
Cimatu
,
J. Phys. Chem. C
123
,
28201
(
2019
).
61.
H. F.
Wang
,
W.
Gan
,
R.
Lu
,
Y.
Rao
, and
B. H.
Wu
,
Int. Rev. Phys. Chem.
24
,
191
(
2005
).
62.
S.
Ye
,
P.
Majumdar
,
B.
Chisholm
,
S.
Stafslien
, and
Z.
Chen
,
Langmuir
26
,
16455
(
2010
).
63.
T. T.
Bui
,
L. A.
Colón
, and
L.
Velarde
,
J. Phys. Chem. Lett.
12
,
5695
(
2021
).
64.
K.
Tian
and
S.
Ye
,
J. Phys. Chem. C
119
,
25394
(
2015
).
65.
Z.
Liu
,
M.
Liu
,
Y.
Liu
,
C.
Zhang
,
X.
Wang
,
L.
Ma
,
H.
Cai
, and
Q.
Cheng
,
Appl. Surf. Sci.
521
,
146364
(
2020
).
66.
K.
Tian
,
B.
Zhang
,
S.
Ye
, and
Y.
Luo
,
J. Phys. Chem. C
119
,
16587
(
2015
).
67.
J.-S.
Sin
,
Y.-M.
Jang
,
C.-H.
Kim
, and
H.-C.
Kim
,
AIP Adv.
8
,
105222
(
2018
).
68.
J. J.
Karnes
and
I.
Benjamin
,
J. Phys. Chem. B
125
,
3629
(
2021
).
69.
S.
Pothoczki
,
A.
Ottochian
,
M.
Rovira-Esteva
,
L.
Pardo
,
J. L.
Tamarit
, and
G.
Cuello
,
Phys. Rev. B
85
,
014202
(
2012
).
70.
Y.
Levy
and
J. N.
Onuchic
,
Annu. Rev. Biophys. Biomol. Struct.
35
,
389
(
2006
).
71.
M.
Heyden
,
J. Chem. Phys.
150
,
094701
(
2019
).
72.
S.
Strazdaite
,
J.
Versluis
,
E. H.
Backus
, and
H. J.
Bakker
,
J. Chem. Phys.
140
,
054711
(
2014
).
73.
M.
Sovago
,
R. K.
Campen
,
G. W.
Wurpel
,
M.
Müller
,
H. J.
Bakker
, and
M.
Bonn
,
Phys. Rev. Lett.
100
,
173901
(
2008
).
74.
S. N.
Wren
,
B. P.
Gordon
,
N. A.
Valley
,
L. E.
McWilliams
, and
G. L.
Richmond
,
J. Phys. Chem. A
119
,
6391
(
2015
).
75.
N.
Watanabe
,
K.
Suga
, and
H.
Umakoshi
,
J. Chem.
2019
,
4867327
.
76.
S.
Pal
and
A.
Chattopadhyay
,
J. Phys. Chem. Lett.
12
,
9697
(
2021
).

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