We visualized a dynamic process of fatty acid uptake of brown adipocytes using a time-lapse ultra-broadband multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging system with an onstage incubator. Combined with the deuterium labeling technique, the intracellular uptake of saturated fatty acids was traced up to 9 h, a substantial advance over the initial multiplex CARS system, with an analysis time of 80 min. Characteristic metabolic activities of brown adipocytes, such as resistance to lipid saturation, were elucidated, supporting the utility of the newly developed system.
Visualization of intracellular lipid metabolism in brown adipocytes by time-lapse ultra-multiplex CARS microspectroscopy with an onstage incubator
Present address: Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, 5-10-1 Fuchinobe, Chuo-Ku, Sagamihara-shi, Kanagawa 252-5258 Japan.
Note: This paper is part of the JCP Special Topic on Chemical Imaging.
Yuki Takei, Rie Hirai, Aya Fukuda, Shinichi Miyazaki, Rintaro Shimada, Yuko Okamatsu-Ogura, Masayuki Saito, Philippe Leproux, Koji Hisatake, Hideaki Kano; Visualization of intracellular lipid metabolism in brown adipocytes by time-lapse ultra-multiplex CARS microspectroscopy with an onstage incubator. J. Chem. Phys. 28 September 2021; 155 (12): 125102. https://doi.org/10.1063/5.0063250
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