The design and characterization of a new laser-desorption molecular beam source, tailored for use in x-ray free-electron laser and ultrashort-pulse laser imaging experiments, is presented. It consists of a single mechanical unit containing all source components, including the molecular-beam valve, the sample, and the fiber-coupled desorption laser, which is movable in five axes, as required for experiments at central facilities. Utilizing strong-field ionization, we characterize the produced molecular beam and evaluate the influence of desorption laser pulse energy, relative timing of valve opening and desorption laser, sample bar height, and which part of the molecular packet is probed on the sample properties. Strong-field ionization acts as a universal probe and allows detecting all species present in the molecular beam, and hence enables us to analyze the purity of the produced molecular beam, including molecular fragments. We present optimized experimental parameters for the production of the purest molecular beam, containing the highest yield of intact parent ions, which we find to be very sensitive to the placement of the desorbed-molecule plumes within the supersonic expansion.
REFERENCES
1.
F. J.
Vastola
and A. J.
Pirone
, “Ionization of organic solids by laser irradiation
,” Adv. Mass Spectrom.
4
, 107
(1968
).2.
A. M.
Rijs
and J.
Oomens
, “IR spectroscopic techniques to study isolated biomolecules
,” in Gas-Phase IR Spectroscopy and Structure of Biological Molecules
, edited by A. M.
Rijs
and J.
Oomens
(Springer Verlag
, 2015
), Chap. 1, pp. 1
–42
.3.
R.
Tembreull
and D. M.
Lubman
, “Resonant two-photon ionization of small peptides using pulsed laser desorption in supersonic beam mass spectrometry
,” Anal. Chem.
59
, 1003
–1006
(1987
).4.
G.
Meijer
, M. S.
de Vries
, H. E.
Hunziker
, and H. R.
Wendt
, “Laser desorption jet-cooling of organic molecules–Cooling characteristics and detection sensitivity
,” Appl. Phys. B
51
, 395
–403
(1990
).5.
E.
Nir
, K.
Kleinermanns
, and M. S.
de Vries
, “Pairing of isolated nucleic-acid bases in the absence of the DNA backbone
,” Nature
408
, 949
–951
(2000
).6.
M. S.
de Vries
and P.
Hobza
, “Gas-phase spectroscopy of biomolecular building blocks
,” Annu. Rev. Phys. Chem.
58
, 585
–612
(2007
).7.
S.-i.
Ishiuchi
, K.
Yamada
, H.
Oba
, H.
Wako
, and M.
Fujii
, “Gas phase ultraviolet and infrared spectroscopy on a partial peptide of β2-adrenoceptor SIVSF-NH2 by a laser desorption supersonic jet technique
,” Phys. Chem. Chem. Phys.
18
, 23277
–23284
(2016
).8.
J. M.
Bakker
, C.
Plützer
, I.
Hünig
, T.
Häber
, I.
Compagnon
, G.
von Helden
, G.
Meijer
, and K.
Kleinermanns
, “Folding structures of isolated peptides as revealed by gas-phase mid-infrared spectroscopy
,” Chem. Phys. Chem.
6
, 120
–128
(2005
).9.
J. M.
Bakker
, L. M.
Aleese
, G.
Meijer
, and G.
von Helden
, “Fingerprint IR spectroscopy to probe amino acid conformations in the gas phase
,” Phys. Rev. Lett.
91
, 203003
(2003
).10.
M. R.
Ligare
, A. M.
Rijs
, G.
Berden
, M.
Kabeláč
, D.
Nachtigallova
, J.
Oomens
, and M. S.
de Vries
, “Resonant infrared multiple photon dissociation spectroscopy of anionic nucleotide monophosphate clusters
,” J. Phys. Chem. B
119
, 7894
–7901
(2015
).11.
J.
Zhang
, L.
Pei
, and W.
Kong
, “Zero kinetic energy photoelectron spectroscopy of tetracene using laser desorption for vaporization
,” J. Chem. Phys.
128
, 104301
(2008
).12.
A.
Barty
, J.
Küpper
, and H. N.
Chapman
, “Molecular imaging using x-ray free-electron lasers
,” Annu. Rev. Phys. Chem.
64
, 415
–435
(2013
).13.
R.
Neutze
, R.
Wouts
, D.
van der Spoel
, E.
Weckert
, and J.
Hajdu
, “Potential for biomolecular imaging with femtosecond x-ray pulses
,” Nature
406
, 752
–757
(2000
).14.
B.
Ziaja
, H. N.
Chapman
, R.
Fäustlin
, S.
Hau-Riege
, Z.
Jurek
, A. V.
Martin
, S.
Toleikis
, F.
Wang
, E.
Weckert
, and R.
Santra
, “Limitations of coherent diffractive imaging of single objects due to their damage by intense x-ray radiation
,” New J. Phys.
14
, 115015
(2012
).15.
U.
Lorenz
, N. M.
Kabachnik
, E.
Weckert
, and I. A.
Vartanyants
, “Impact of ultrafast electronic damage in single-particle x-ray imaging experiments
,” Phys. Rev. E
86
, 051911
(2012
); e-print arXiv:1206.6960 [physics].16.
K.
Nass
, L.
Foucar
, T. R. M.
Barends
, E.
Hartmann
, S.
Botha
, R. L.
Shoeman
, R. B.
Doak
, R.
Alonso-Mori
, A.
Aquila
, S.
Bajt
, A.
Barty
, R.
Bean
, K. R.
Beyerlein
, M.
Bublitz
, N.
Drachmann
, J.
Gregersen
, H. O.
Jönsson
, W.
Kabsch
, S.
Kassemeyer
, J. E.
Koglin
, M.
Krumrey
, D.
Mattle
, M.
Messerschmidt
, P.
Nissen
, L.
Reinhard
, O.
Sitsel
, D.
Sokaras
, G. J.
Williams
, S.
Hau-Riege
, N.
Timneanu
, C.
Caleman
, H. N.
Chapman
, S.
Boutet
, and I.
Schlichting
, “Indications of radiation damage in ferredoxin microcrystals using high-intensity X-FEL beams
,” J. Synchrotron Radiat.
22
, 225
–238
(2015
).17.
J.
Küpper
, S.
Stern
, L.
Holmegaard
, F.
Filsinger
, A.
Rouzée
, A.
Rudenko
, P.
Johnsson
, A. V.
Martin
, M.
Adolph
, A.
Aquila
, S.
Bajt
, A.
Barty
, C.
Bostedt
, J.
Bozek
, C.
Caleman
, R.
Coffee
, N.
Coppola
, T.
Delmas
, S.
Epp
, B.
Erk
, L.
Foucar
, T.
Gorkhover
, L.
Gumprecht
, A.
Hartmann
, R.
Hartmann
, G.
Hauser
, P.
Holl
, A.
Hömke
, N.
Kimmel
, F.
Krasniqi
, K.-U.
Kühnel
, J.
Maurer
, M.
Messerschmidt
, R.
Moshammer
, C.
Reich
, B.
Rudek
, R.
Santra
, I.
Schlichting
, C.
Schmidt
, S.
Schorb
, J.
Schulz
, H.
Soltau
, J. C. H.
Spence
, D.
Starodub
, L.
Strüder
, J.
Thøgersen
, M. J. J.
Vrakking
, G.
Weidenspointner
, T. A.
White
, C.
Wunderer
, G.
Meijer
, J.
Ullrich
, H.
Stapelfeldt
, D.
Rolles
, and H. N.
Chapman
, “X-ray diffraction from isolated and strongly aligned gas-phase molecules with a free-electron laser
,” Phys. Rev. Lett.
112
, 083002
(2014
); e-print arXiv:1307.4577 [physics].18.
S.
Stern
, L.
Holmegaard
, F.
Filsinger
, A.
Rouzée
, A.
Rudenko
, P.
Johnsson
, A. V.
Martin
, A.
Barty
, C.
Bostedt
, J. D.
Bozek
, R. N.
Coffee
, S.
Epp
, B.
Erk
, L.
Foucar
, R.
Hartmann
, N.
Kimmel
, K.-U.
Kühnel
, J.
Maurer
, M.
Messerschmidt
, B.
Rudek
, D. G.
Starodub
, J.
Thøgersen
, G.
Weidenspointner
, T. A.
White
, H.
Stapelfeldt
, D.
Rolles
, H. N.
Chapman
, and J.
Küpper
, “Toward atomic resolution diffractive imaging of isolated molecules with x-ray free-electron lasers
,” Faraday Discuss.
171
, 393
(2014
); e-print arXiv:1403.2553 [physics].19.
J. M.
Glownia
, A.
Natan
, J. P.
Cryan
, R.
Hartsock
, M.
Kozina
, M. P.
Minitti
, S.
Nelson
, J.
Robinson
, T.
Sato
, T.
van Driel
, G.
Welch
, C.
Weninger
, D.
Zhi
, and P. H.
Bucksbaum
, “Self-referenced coherent diffraction x-ray movie of angstrom- and femtosecond-scale atomic motion
,” Phys. Rev. Lett.
117
, 153003
(2016
); e-print arXiv:1608.03039 [physics].20.
F.
Calegari
, D.
Ayuso
, A.
Trabattoni
, L.
Belshaw
, S.
De Camillis
, S.
Anumula
, F.
Frassetto
, L.
Poletto
, A.
Palacios
, P.
Decleva
, J. B.
Greenwood
, F.
Martín
, and M.
Nisoli
, “Ultrafast electron dynamics in phenylalanine initiated by attosecond pulses
,” Science
346
, 336
–339
(2014
).21.
B.
Yan
, S.
Jaeqx
, W. J.
van der Zande
, and A. M.
Rijs
, “A conformation-selective IR-UV study of the dipeptides Ac-Phe-Ser-NH2 and Ac-Phe-Cys-NH2: Probing the SHO and OHO hydrogen bond interactions
,” Phys. Chem. Chem. Phys.
16
, 10770
–10778
(2014
).22.
M.
Alauddin
, H. S.
Biswal
, E.
Gloaguen
, and M.
Mons
, “Intra-residue interactions in proteins: Interplay between serine or cysteine side chains and backbone conformations, revealed by laser spectroscopy of isolated model peptides
,” Phys. Chem. Chem. Phys.
17
, 2169
–2178
(2015
).23.
Y.-P.
Chang
, D. A.
Horke
, S.
Trippel
, and J.
Küpper
, “Spatially-controlled complex molecules and their applications
,” Int. Rev. Phys. Chem.
34
, 557
–590
(2015
); e-print arXiv:1505.05632 [physics].24.
D.
Irimia
, D.
Dobrikov
, R.
Kortekaas
, H.
Voet
, D. A.
van den Ende
, W. A.
Groen
, and M. H. M.
Janssen
, “A short pulse (7 μs FWHM) and high repetition rate (dc–5kHz) cantilever piezovalve for pulsed atomic and molecular beams
,” Rev. Sci. Instrum.
80
, 113303
(2009
).25.
K.
Luria
, W.
Christen
, and U.
Even
, “Generation and propagation of intense supersonic beams
,” J. Phys. Chem. A
115
, 7362
–7367
(2011
).26.
H. L.
Bethlem
, M. R.
Tarbutt
, J.
Küpper
, D.
Carty
, K.
Wohlfart
, E. A.
Hinds
, and G.
Meijer
, “Alternating gradient focusing and deceleration of polar molecules
,” J. Phys. B
39
, R263
–R291
(2006
); e-print arXiv:0604020 [physics].27.
E. R.
Abraham
and E. A.
Cornell
, “Teflon feedthrough for coupling optical fibers into ultrahigh vacuum systems
,” Appl. Opt.
37
, 1762
–1763
(1998
).28.
A. T. J. B.
Eppink
and D. H.
Parker
, “Velocity map imaging of ions and electrons using electrostatic lenses: Application in photoelectron and photofragment ion imaging of molecular oxygen
,” Rev. Sci. Instrum.
68
, 3477
–3484
(1997
).29.
E. A.
Rohlfing
, D. M.
Cox
, and A.
Kaldor
, “Production and characterization of supersonic carbon cluster beams
,” J. Chem. Phys.
81
, 3322
–3330
(1984
).30.
C. R.
Calvert
, L.
Belshaw
, M. J.
Duffy
, O.
Kelly
, R. B.
King
, A. G.
Smyth
, T. J.
Kelly
, J. T.
Costello
, D. J.
Timson
, W. A.
Bryan
, T.
Kierspel
, P.
Rice
, I. C. E.
Turcu
, C. M.
Cacho
, E.
Springate
, I. D.
Williams
, and J. B.
Greenwood
, “LIAD-fs scheme for studies of ultrafast laser interactions with gas phase biomolecules
,” Phys. Chem. Chem. Phys.
14
, 6289
–6297
(2012
).31.
P.
Arrowsmith
, M. S.
de Vries
, H. E.
Hunziker
, and H. R.
Wendt
, “Pulsed laser desorption near a jet orifice: Concentration profiles of entrained perylene vapor
,” Appl. Phys. B
46
, 165
–173
(1988
).32.
D. A.
Horke
, N.
Roth
, L.
Worbs
, and J.
Küpper
, “Characterizing gas flow from aerosol particle injectors
,” J. Appl. Phys.
121
, 123106
(2017
); e-print arXiv:1609.09020 [physics].33.
F.
Piuzzi
, I.
Dimicoli
, M.
Mons
, B.
Tardivel
, and Q. C.
Zhao
, “A simple laser vaporization source for thermally fragile molecules coupled to a supersonic expansion: Application to the spectroscopy of tryptophan
,” Chem. Phys. Lett.
320
, 282
–288
(2000
).34.
F.
Filsinger
, U.
Erlekam
, G.
von Helden
, J.
Küpper
, and G.
Meijer
, “Selector for structural isomers of neutral molecules
,” Phys. Rev. Lett.
100
, 133003
(2008
); e-print arXiv:0802.2795 [physics].© 2017 Author(s).
2017
Author(s)
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