Randomly rotating particles that have been isotropically labeled with rigidly linked fluorophores will undergo non-isotropic (patchy) photobleaching under illumination due to the dipole coupling of fluorophores with light. For a rotational diffusion rate D of the particle and a photobleaching time scale of the fluorophores, the dynamics of this process are characterized by the dimensionless combination . We find significant interparticle fluctuations at intermediate . These fluctuations vanish at both large and small or at small or large elapsed times t. Associated with these fluctuations between particles, we also observe transient non-monotonicities of the brightness of individual particles. These non-monotonicities can be as much as 20% of the original brightness. We show that these novel photobleach-fluctuations dominate over variability of single-fluorophore orientation when there are at least 103 fluorophores on individual particles.
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14 September 2017
Research Article|
September 12 2017
Photobleaching of randomly rotating fluorescently decorated particles
Swadhin Taneja;
Swadhin Taneja
a)
Department of Physics and Atmospheric Science, Dalhousie University
, Halifax, Nova Scotia B3H 4R2, Canada
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Andrew D. Rutenberg
Andrew D. Rutenberg
b)
Department of Physics and Atmospheric Science, Dalhousie University
, Halifax, Nova Scotia B3H 4R2, Canada
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a)
Electronic mail: swadhin.taneja@dal.ca
b)
Electronic mail: andrew.rutenberg@dal.ca
J. Chem. Phys. 147, 104105 (2017)
Article history
Received:
June 11 2017
Accepted:
August 28 2017
Citation
Swadhin Taneja, Andrew D. Rutenberg; Photobleaching of randomly rotating fluorescently decorated particles. J. Chem. Phys. 14 September 2017; 147 (10): 104105. https://doi.org/10.1063/1.4989673
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