Salt, glycerol, and dimethyl sulfoxide (DMSO) are used to modify the properties of protein solutions. We experimentally determined the effect of these additives on the phase behavior of lysozyme solutions. Upon the addition of glycerol and DMSO, the fluid–solid transition and the gas–liquid coexistence curve (binodal) shift to lower temperatures and the gap between them increases. The experimentally observed trends are consistent with our theoretical predictions based on the thermodynamic perturbation theory and the Derjaguin-Landau-Verwey-Overbeek model for the lysozyme-lysozyme pair interactions. The values of the parameters describing the interactions, namely the refractive indices, dielectric constants, Hamaker constant and cut-off length, are extracted from literature or are experimentally determined by independent experiments, including static light scattering, to determine the second virial coefficient. We observe that both, glycerol and DMSO, render the potential more repulsive, while sodium chloride reduces the repulsion.
Skip Nav Destination
Article navigation
7 January 2012
Research Article|
January 04 2012
Effect of glycerol and dimethyl sulfoxide on the phase behavior of lysozyme: Theory and experiments
Christoph Gögelein;
Christoph Gögelein
1
Max-Planck-Institut für Dynamik und Selbstorganisation
, Am Faßberg 17, 37077 Göttingen, Germany
Search for other works by this author on:
Dana Wagner;
Dana Wagner
b)
2Condensed Matter Physics Laboratory,
Heinrich-Heine-University
, Universitätsstraße 1, 40225 Düsseldorf, Germany
Search for other works by this author on:
Frédéric Cardinaux;
Frédéric Cardinaux
2Condensed Matter Physics Laboratory,
Heinrich-Heine-University
, Universitätsstraße 1, 40225 Düsseldorf, Germany
Search for other works by this author on:
Gerhard Nägele;
Gerhard Nägele
3
Institute of Complex Systems (ICS-3)
, Forschungszentrum Jülich, 52425 Jülich, Germany
Search for other works by this author on:
Stefan U. Egelhaaf
Stefan U. Egelhaaf
2Condensed Matter Physics Laboratory,
Heinrich-Heine-University
, Universitätsstraße 1, 40225 Düsseldorf, Germany
Search for other works by this author on:
a)
Electronic mail: christoph.goegelein@ds.mpg.de.
b)
Christoph Gögelein and Dana Wagner contributed equally to this work.
J. Chem. Phys. 136, 015102 (2012)
Article history
Received:
October 27 2011
Accepted:
December 06 2011
Citation
Christoph Gögelein, Dana Wagner, Frédéric Cardinaux, Gerhard Nägele, Stefan U. Egelhaaf; Effect of glycerol and dimethyl sulfoxide on the phase behavior of lysozyme: Theory and experiments. J. Chem. Phys. 7 January 2012; 136 (1): 015102. https://doi.org/10.1063/1.3673442
Download citation file:
Sign in
Don't already have an account? Register
Sign In
You could not be signed in. Please check your credentials and make sure you have an active account and try again.
Sign in via your Institution
Sign in via your InstitutionPay-Per-View Access
$40.00
Citing articles via
Related Content
Dimethyl sulfoxide induced structural transformations and non-monotonic concentration dependence of conformational fluctuation around active site of lysozyme
J. Chem. Phys. (March 2012)
Heterogeneity in binary mixtures of dimethyl sulfoxide and glycerol: Fluorescence correlation spectroscopy
J. Chem. Phys. (June 2013)
Comparison of the low‐temperature crystallization of glasses in the ternary systems H2O‐NaCl‐dimethyl sulfoxide and H2O‐NaCl‐glycerol
Journal of Applied Physics (September 2008)
Protein phase behavior and crystallization: Effect of glycerol
J. Chem. Phys. (September 2007)