A new analytical approach was developed to characterize the properties of water molecules bound to macromolecules in solution using nuclear magnetic resonance (NMR) relaxation. A combination of conventional (single-quantum) and triple-quantum filtered Hahn echo and inversion recovery measurements was employed. From measured relaxation rate constants, the fraction and the correlation time of bound molecules and the relaxation rate constant of bulk water in solution were calculated. This was done by solving analytically a set of nonlinear equations describing the overall relaxation rate constants in the presence of chemical exchange between bulk and bound water. The analytical approach shows the uniqueness of the solution for a given set of three relaxation rate constants. This important result sheds light on the data reduction problem from NMR experiments on biological systems. Water bound in photosystem I isolated from the wild type and rubA variant of the cyanobacterium Synechocystis species PCC 7002 was investigated for the first time. The analysis revealed that photosystem I isolated from the wild type binds water molecules, whereas photosystem I isolated from the rubA variant binds only . The accuracy of the method proposed can be increased by further enrichment. The methodology, established for the first time in this work, allows the study of a diverse range of biological samples regardless of their size and molecular weight. Applied initially to photosystem I, this novel method has important consequences for the future investigation of the assembly of biological molecules.
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7 January 2008
Research Article|
January 02 2008
Investigation of water bound to photosystem I with multiquantum filtered nuclear magnetic resonance
M. Krzystyniak;
M. Krzystyniak
Institute of Chemistry, Sekr. C 2,
Technische Universität Berlin
, Strasse des 17 Juni 135, D-10623 Berlin, Germany and Fachbereich Physik, Institut für Experimental Physik (WE 1), Freie Universität Berlin
, Arnimallee 14, D-14195 Berlin, Germany
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Gaozhong Shen;
Gaozhong Shen
Department of Biochemistry and Molecular Biology,
The Pennsylvania State University
, University Park, Pennsylvania 16802, USA
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John H. Golbeck;
John H. Golbeck
Department of Biochemistry and Molecular Biology,
The Pennsylvania State University
, University Park, Pennsylvania 16802, USA and Department of Chemistry, The Pennsylvania State University
, University Park, Pennsylvania 16802, USA
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Mikhail L. Antonkine
Mikhail L. Antonkine
Fachbereich Physik, Institut für Experimental Physik (WE 1),
Freie Universität Berlin
, Arnimallee 14, D-14195 Berlin, Germany and Max-Planck Institut für Bioanorganische Chemie Stiftstrasse
, 34-36/D-45470 Mülheim an der Ruhr, Germany
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J. Chem. Phys. 128, 014503 (2008)
Article history
Received:
April 18 2007
Accepted:
October 25 2007
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Citation
M. Krzystyniak, Gaozhong Shen, John H. Golbeck, Mikhail L. Antonkine; Investigation of water bound to photosystem I with multiquantum filtered nuclear magnetic resonance. J. Chem. Phys. 7 January 2008; 128 (1): 014503. https://doi.org/10.1063/1.2813891
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