In this study, the temporal distribution of the photobleaching of tetramethylrhodamine molecules embedded in agarose gel is measured using a single molecule detection technique equipped with a confocal microscope and a multichannel scaler. The result shows the exponential photobleaching time distribution at long time scales and the average photobleaching time of 46.1±3.8 ms for a given excitation intensity of about 8 kW/cm2.

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