Pump/probe self-heterodyne experiments on the amide I band of a small de novo cyclic pentapeptide were utilized to demonstrate a novel form of two-dimensional (2D) vibrational spectroscopy. Spectrally resolved cross peaks are observed, which measure the coupling between different peptide units and which can be related to the structure of the peptide, in analogy to 2D-NMR spectroscopy. In contrast to our previous work, these experiments work in the time domain in the semiimpulsive limit, employing two intense ultrashort infrared laser pulses. A theoretical formalism is presented in order to model the interstate coherent wave packet generated by the excitation pulse and the resulting spectroscopic signal. The observed coherences provide an independent proof of excitonic coupling within the amide I manifold of the peptide backbone.
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22 January 2000
Research Article|
January 22 2000
Pump/probe self heterodyned 2D spectroscopy of vibrational transitions of a small globular peptide
Peter Hamm;
Peter Hamm
Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6323
Max Born Institut, 12489 Berlin, Germany
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Manho Lim;
Manho Lim
Department of Chemistry and Department of Biophysics and Biochemistry, Johnson Foundation, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6323
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William F. DeGrado;
William F. DeGrado
Department of Chemistry and Department of Biophysics and Biochemistry, Johnson Foundation, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6323
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Robin M. Hochstrasser
Robin M. Hochstrasser
Department of Chemistry and Department of Biophysics and Biochemistry, Johnson Foundation, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6323
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J. Chem. Phys. 112, 1907–1916 (2000)
Article history
Received:
July 19 1999
Accepted:
October 26 1999
Citation
Peter Hamm, Manho Lim, William F. DeGrado, Robin M. Hochstrasser; Pump/probe self heterodyned 2D spectroscopy of vibrational transitions of a small globular peptide. J. Chem. Phys. 22 January 2000; 112 (4): 1907–1916. https://doi.org/10.1063/1.480772
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