Frontiers of magnetic force microscopy

First demonstrated in 1987,^1,2 magnetic force microscopy (MFM) is a well-established and widely used technique. Over the last three decades, the method has been extensively used in a vast number of applications, where the knowledge of the local distribution of the magnetic properties of thin film materials and their nanostructures is of paramount importance. This functional technique relies on quantifying the long-range magnetostatic force between the magnetic sample and a magnetically coated probe positioned at a constant height over the specimen surface. In its simplest form, the typical MFM procedure involves two linear scans. First, the topography of the surface is obtained by using tapping mode atomic force microscopy (AFM) (i.e., exploiting van der Waals interactions between the probe and sample). During the second scan, the probe is lifted away from the sample [i.e., van der Waals interactions are negligible and the probe experiences only long-range magnetic (and electrostatic) interactions] and the initial topography profile is repeated at the constant lift scan height [Fig. 1(a)].

The knowledge and expertise accumulated in the initial period of MFM development established a fundamental base for the modern commercial MFM systems. However until recently, unlike other functional scanning probe microscopy (SPM) techniques, commercial MFM systems have not demonstrated a variety of modes and were used primarily on their own. At the same time, the use of MFM was somewhat limited to “classic” ferromagnetic (FM) samples, although they were represented in a variety of forms. Recently, the rise of novel materials, often combining magnetic and other functional properties or demonstrating complex forms of magnetism, such as multiferroics, topological insulators (TIs), and magnetic semiconductors, has stimulated a burst in the development of advanced MFM modes.

A number of methods have been developed to image magnetic structures with different sensitivities and on many lateral scales. These methods can be roughly divided into beam- and scanning probe-based techniques. The former involves a broad spectrum of physical principles of operation (i.e., polarized light, x-rays, and electrons) and includes both well-established and novel techniques such as magneto-optical microscopy based on Kerr and Faraday effects,^3,4 Lorentz force microscopy,⁵ scanning electron microscopy (SEM) with polarization analysis,^6,7 and photoemission electron microscopy,^8,9 specifically including x-ray magnetic linear and circular dichroism microscopy.¹⁰ 

The latter group comprises a variety of magnetically sensitive SPM-based techniques. One of the recent exciting examples includes integration of nitrogen vacancy (NV) defect centers with high-Q diamond mechanical oscillators, allowing realization of a quantum qubit system with the advantages of highly coherent electron spin and narrow optical transitions, accompanied by nanometer scale resolution.^11,12 Another example is magnetic resonance force microscopy that succeeded in detecting single electrons and small nuclear spin ensembles.^13,14 Successful examples of mounting a magnetic sensor on a scanning probe include Hall probe microscopy¹⁵ and superconducting quantum interference device (SQUID) microscopy.^16–18 All these methods have both advantages and drawbacks, as well as a different degree of application in research and industry. These techniques are, however, beyond the scope of this Perspective article, which will entirely focus on MFM.

MFM has been most widely used for the local characterization of magnetic nanostructures and imaging the magnetic field distribution at the surface of magnetic materials.^1,2,19 Despite decades of advances in magnetic imaging,²⁰ obtaining direct, uncoupled, and quantitative information with high spatial resolution remains an outstanding challenge.

Among all methods for the observation of magnetic domain structures, MFM is the most widely used, due to its high spatial resolution (down to ∼10 nm),²¹ sensitivity (∼10 pN),²² relative simplicity in sample preparation, capability to apply in situ magnetic fields to study magnetization processes,²³ and its ability to operate in different environments.²⁴ The MFM technique has been proven as an excellent characterization tool in both fundamental research and industrial applications. For comprehensive MFM reviews performed in the early days of MFM, see Refs. 21, 25, and 26.

The aim of this Perspective article is to analyze recent progress in the development of MFM, present the current state-of-the-art, and outline the future and perspective of this fascinating field. Such emerging aspects as probe-engineering alternatives, new instrumentation, quantitative measurements, the correct interpretation of the resulting MFM images, the loss of energy analysis and applications of MFM to new emerging areas of the material science, physics and biology, etc. are subjects of ongoing research that will be discussed in this work.

The article is organized as follows: Sec. II describes the physical principles of MFM, and common artifacts in MFM measurement; the review (Sec. III) describes the recent developments in instrumentation and the main application areas; finally, this Perspective article (Sec. IV) presents the emerging trends in the field of MFM.

Despite the advantages highlighted throughout this article, MFM is not without its limitations and errors. Just like other SPM techniques, MFM is susceptible to artifacts, which can perturb the measured image and, without careful handling, can lead to incorrect interpretation of the results. Many common SPM-based artifacts and methods for reducing their effects are discussed elsewhere.³⁴  Table I summarizes MFM-specific artifacts and solutions to minimize their effects on recorded images. A number of these will be specifically discussed throughout the present work.

Arguably the most important factor for accurately representing stray magnetic fields emanating from the measurand is careful consideration of the probe and its own magnetic and physical properties relative to the sample. The resolution and sensitivity of MFM probes are primarily governed by the tip's shape and magnetic properties. However, as an MFM image is a convolution of both the sample and the probe's magnetic properties, it is imperative to also consider the induced effects of the probe and sample's stray-field on each other, as this can result in imaging artifacts, such as altering the moment of either the sample or the probe.^35,36 For example, Fig. 2 shows that the magnetic state of a low coercivity Ni disk can be perturbed by the MFM probe with higher magnetic moment [standard moment (SM)] during data acquisition, compared to the low moment (LM) probe [(a) and (b), respectively].

Another common artifact in MFM data acquisition is the effects of both induced electrostatic interactions between the probe and the sample, and magnetic contamination. There are a few examples of misinterpreted MFM images in literature due to these parasitic artifacts, including proposed magnetic highly ordered pyrolytic graphite (HOPG)³⁷ (demonstrated that observed contrast was due to electrostatics, i.e., not a magnetic origin, by Martínez-Martín et al.³⁸) and room temperature ferromagnetism in C₆₀ polymers^39,40 [shown to be Fe₃C contamination^41,42 and later retracted by (most) of the original authors⁴³]. Thus, for magnetic contamination, it is vital to carefully monitor the magnetic history and exclude exposure of magnetic materials and tools (e.g., catalysts, tweezers, etc.) to the sample in fabrication/handling processes prior to the measurement. In the case of parasitic electrostatics, it is crucial to be able to identify and nullify the adverse artifacts. For this, it is primarily important to consider the electrical grounding during the measurement, with alternative active and passive methods for nullifying the effects discussed later in Sec. III.

Here, we describe the recent developments in instrumentation, quantitative MFM (qMFM) modeling, and modern application areas of MFM. Specifically, we address such areas of instrumentational development as in-field and low/high temperature MFM and discuss the compensation of electrostatic signals and energy dissipation in MFM. We also briefly present different types of MFM, i.e., dynamic magneto-electric force microscopy (MeFM), bimodal MFM, and magnetic scanning gate microscopy (mSGM), as well as the development of custom-designed MFM probes. The modern objects of MFM studies discussed here include (ultra-)thin films with perpendicular magnetic anisotropy (PMA), artificial spin ice as an example of patterned structures, magnetic topological structures, multiferroic materials, and materials for Life Science applications.

Only a few years after the initial development of MFM, different groups explored the power of MFM imaging with in situ applied magnetic fields. Initially, custom-built approaches (typically consisting of a system of coils or permanent magnets) were implemented in commercial MFM equipment with in-plane (IP) or out-of-plane (OOP) field for maximum amplitudes ranging from 300 to 500 mT. At that time, hot topics included the evaluation of switching fields of sub-micron magnetic patterns⁵⁸ and the study of the magnetization reversal both in thin films with perpendicular magnetic anisotropy (PMA)^59,60 and in magnetic nanowires (NWs)^61,62 (with OOP and IP fields, respectively). As the available range of magnetic fields progressed (up to 1000 mT), it became possible to study the magnetization process in materials touted for magnetic recording media.^63,64 Specialized custom MFM systems with in field capabilities operating under extreme conditions (7 T OOP at 7.5 K and UHV) were reported, e.g., Kappenberger et al.,⁶⁵ and used currently for probing novel nanomagnetism, e.g., exchange bias multilayers.^66,67 Moreover, the application of vector magnetic fields in MFM was recently demonstrated.²¹ 

In addition to the standard MFM images recorded at fixed magnetic fields, two different groups developed in situ MFM imaging in variable field, where the probe scans along one spatial dimension, while the slow axis of the scan corresponds to a gradual change of the magnetic field.^21,68 This variable field MFM mode allows for the evaluation of the critical fields in individual magnetic elements or the coercive field of the MFM probes.

The in-field MFM technique provides a reliable understanding of the internal spin structure and its magnetization reversal processes and has been recently applied to studies of both the domain configuration and domain wall (DW) properties in various magnetic thin films and nano-objects.^69–71 For example, in-field MFM has been used to characterize the novel spin configuration and the magnetization mechanism in cylindrical magnetic NWs, which are exempt of the Walker breakdown limit that restricts the DW velocity.^72,73 The in-field MFM technique is also paramount for studies of the topologically protected magnetic states characterized by the Dzyaloshinskii–Moriya interaction (DMI), e.g., magnetic skyrmions, since this technique is being intensively used to analyze their stability, nucleation, and propagation.^74–78 

The combination of nanomagnetism and biomedical applications has also been a hot topic in recent years, e.g., in application to studies of hyperthermia effect for cancer treatment. The study of individual magnetic nanoparticles (MNPs) by in-field MFM allows for determination of the easy axis of Fe_3–xO₄ MNPs^79,80 and the vortex state formation and annihilation in individual 25 nm MNPs.⁶⁷ 

Variable temperature MFM is another important topic for MFM development. Low-temperature MFM has been utilized to study superconducting flux vortices in Type II superconductors, where detailed information on the temperature and field dependence⁸¹ of their properties can be obtained with the high spatial resolution of the MFM. Understanding phenomena such as flux creep and pinning⁸² at the nanoscale is important for technological applications such as high critical temperature (high-T_c) superconducting ceramics, where creep can cause a reduction in the critical current and fields.^82,83 Low-temperature MFM measurements in the range of 7.6–80 K have been used to image flux vortices in YBa₂Cu₃O_7–x (YBCO) single crystal films.⁸⁴ In these experiments, the authors used a bath cryostat with a variable temperature insert and a superconducting magnet. This system allows for measurements with a temperature range of 6–400 K, ultra-high vacuum, and applied fields of 7 T. The same authors also demonstrated how vortex bundles can be manipulated and nucleated using the stray field from the magnet probe.⁸⁵ 

While piezo excitation is the most common way to excite AFM cantilevers, it is not specifically advantageous in low temperature systems, where instabilities originate from the thermal contraction of mechanical parts pressing the cantilever. In the past, the photothermal excitation of the cantilevers using two laser sources was accepted to be the best alternative method. In this, one of the beams was focused at the end of the cantilever for deflection measurement, and the second beam near the base of the cantilever for excitation.^86,87

Recently, a novel radiation pressure based cantilever excitation method for imaging in dynamic AFM mode was presented by Çelik et al.⁸⁸ In order to simplify the optical design in cryogenic AFM/MFM, the authors explored the use of a single laser beam and fiber optic interferometry, both for the excitation and detection of cantilever deflection in AFM imaging. The high performance of the radiation pressure excitation in AFM/MFM was demonstrated by magnetic domains in Co/Pt multilayers and an Abrikosov vortex lattice in BSCCO(2212) single crystal at 4 K.⁸⁸ 

In addition to low-temperature measurements, it is also possible to image magnetic phenomena and transitions that occur at higher temperatures. Typically, these measurements are performed using Peltier or, for higher temperatures, resistive heaters, which can provide in situ measurement environments from room temperature to 520 K. It has been demonstrated that the temperature dependence of the domain structure of FePt thin films can be imaged. This is highly pertinent for future magnetic recording technologies such as heat assisted magnetic recording (HAMR),⁸⁹ where the energy required for magnetization reversal is reduced through near-field laser heating.

FeRh undergoes a first order metamagnetic phase transition from an antiferromagnet to a ferromagnet above a critical temperature of approximately 370 K, which is also accompanied by an expansion of the crystal lattice and a sharp drop in the electrical resistivity.⁹⁰ It has been shown that the control of the electrical resistivity in FeRh can be achieved via strain modulation of a (001) PMN-ZT piezoelectric substrate.⁹¹ This strain modifies the relative contributions to the total electrical resistivity by modifying the relative volume of the antiferromagnetic and FM regions through the strain induced phase transition. In this work, MFM was used to investigate the first order metamagnetic phase transition of FeRh at temperatures above and below the phase transition. It was found that the relative size of the FM domains expands rapidly through the phase transition and then reduces in size upon cooling, highlighting the effectiveness of MFM to gain insight to the magnetic landscape of complex systems on micrometric length scales.

In MFM experiments such as those already discussed, it is important to consider the electrostatic influences to the MFM signal. Here, we discuss further the instrumental developments and examples relevant to separation and compensation of electrostatic signals in MFM. At typical probe–sample working distances, the magnetic and electrostatic interactions can have comparable values depending on the electric and magnetic properties of the system. An electrostatic contribution is present whenever the probe and sample exhibit different work functions, which results in a contact potential difference (CPD). Such electrostatic interaction can mask other long or short range interactions.^21,92 In a homogeneous sample, the CPD can be compensated by applying an appropriate bias voltage between the probe and the sample. However, if the surface is composed of more than one material, this simple method is not applicable,²¹ since the CPD values vary all over the surface. When a heterogeneous sample (e.g., nanostructures on a substrate) is studied, and especially in the case of low magnetic moment materials, it is necessary to consider this problem in order to prevent incorrect image interpretation.³⁷ 

The first method for separating both long range interactions was proposed by Jaafar et al.⁴⁵ There, a combination between Kelvin Probe Force Microscopy and MFM (KPFM/MFM) was used to distinguish between electrostatic and magnetic contributions [Fig. 1(b)]. The method records both the CPD map and the real compensated MFM image, as it cancels the electrostatic interaction between the probe and sample at every point of the image, thus obtaining a pure magnetic signal.

Angeloni et al.⁴⁴ have also demonstrated the effect of electrostatic tip-sample interactions in MFM, which limited the accuracy of magnetic measurements at the nanometer scale. They developed an alternative MFM-based approach, in which the two subsequent images of the same area were collected, one with the probe being magnetized and another with a quasi-demagnetized probe. The MFM map of the true signal is achieved by subtracting the images. Prior to measurement, it is necessary to determine both the remanent saturation and coercivity of the probe by imaging a reference sample with periodically patterned magnetic domains. The authors demonstrated the effectiveness of this technique by characterizing the magnetization curves of individual MNPs.⁹³ 

The ability to distinguish magnetic and electrostatic signals is still open for discussion. Recently, it has been proposed to perform electrostatic force microscopy (EFM) prior to MFM measurements to compare the respective images.⁹⁴ Alternatively, modifying the magnetic state of the sample with an external magnetic field was used to determine whether the origin of the signal is magnetic. However, only by compensating the electrostatic interaction in each point, a true MFM image (and in addition the CPD information) can be obtained in real time.⁴⁵ 

Recently, a number of MFM-related techniques have appeared, each of them designed to address a specific scientific problem, thus having a somewhat narrower application scope than standard MFM. One such specialized MFM-related technique is magneto-electric force microscopy (MeFM). In this mode, the probe is not mechanically driven in the second-pass as in MFM. Instead, a combined AC/DC bias is applied to the sample while the sample potential is electrically modulated at the mechanical resonance of the cantilever. The resulting AC magnetic field from the sample induces the resonant motion of the magnetic probe^95,96 [Fig. 1(c)]. In addition, the probe is electrically isolated and kept at a large constant tip-sample distance during imaging.³⁵ This method is of particular importance for materials exhibiting a strong coupling and interdependence of electrical and magnetic properties and can be employed to detect the electric field-induced magnetization.

MeFM has been used previously to decouple magnetic and electrical effects in complex samples (e.g., 2D electronic liquids³⁵); visualize the magnetoelectric (ME) response from domain patterns in hexagonal manganites^95,96 and antiferromagnetic 180° domains;⁹⁷ and estimate the upper limit of the linear ME coefficient of h-LuFeO₃.⁹⁸ Additionally, many controlled experiments have been undertaken, e.g., a study of MeFM performance in dependence on the modulation frequency,⁹⁶ which showed that lower modulation frequency produces a better signal-to-noise ratio (SNR). However, lower modulation frequency requires longer averaging time to obtain the intrinsic ME response.

Superior aspects and limitations of MeFM were recently summarized by Schöenherr et al.⁹⁷ The advantages include: (i) high lateral resolution with standard/specially formed probes; (ii) ability to resolve and define the DW inclination; (iii) low sensitivity to material inhomogeneities and thus reduced dependence on the associated scattering effect. The current restrictions of MeFM are a relatively small output response and low SNR. Nevertheless, the limited signal can be improved by increasing the electric field, resulting in a larger induced magnetic field; optimizing the temperature to maximize the ME effect response; optimizing the electrodes; the use of probes with higher magnetic moments, leading to a stronger force between the probe and the magnetoelectrically induced magnetic field. The SNR can also be improved by increasing the averaging time per data point or multiple measurements of the same area. It was thus compellingly demonstrated that this advanced technique provides direct visualization of the ME domains and DWs to open up a new paradigm of explorations of emergent mesoscopic phenomena in materials with multiple coupled orders. It was proposed that the method is of utmost importance for exploring emergent phenomena at the mesoscopic scale such as ME coupling in multiferroic domains and DWs, multiferroic skyrmions, or magnetic topological insulators.

Bimodal MFM belongs to the family of multi-frequency SPM. One of the advantages of SPM is the simultaneous detection of a variety of interactions at different probe–sample separations. Multi-frequency SPM is a novel concept that has been developed in the last few years.⁹⁹ These modes are based on the consideration of the microcantilever-based probe as a mechanical system characterized by multiple resonances and harmonics. Each of those frequencies is sensitive to specific information on the sample properties. Appropriately excited and decoded, those frequencies will provide complete information on the electronic and mechanical properties [Fig. 1(d)].

For the particular case of MFM, the multi-frequency techniques have become an active area of research. In 2009, Li et al. presented the bimodal AFM as a technique to simultaneously separate short- and long-range (topographic and magnetic, respectively) forces in ferromagnetic samples.¹⁰⁰ In this work, the cantilever was driven at two flexural resonant modes. Following this idea, Dietz et al. employed the bimodal AFM to measure a nanomechanical effect that enables the detection of ferritin molecules with high lateral resolution and sensitivity.¹⁰¹ More recently, a non-contact bimodal MFM technique operating in vacuum/UHV was developed.¹⁰² In this work, the higher-stiffness second mode is used to map the topography and the magnetic force is measured at first oscillation mode, which is characterized by higher sensitivity. The torsional resonance mode of cantilever oscillation was employed to perform magnetic imaging without topography-related interference and to improve the lateral resolution.¹⁰³ 

Another alternative is to combine a mechanical (1st mode) and electrical excitation (2nd mode) to drive a cantilever. This approach has been explored in the literature to separate electrostatic and magnetic interactions^38,45 or as a tool to control the probe–sample distance.^31,104 In a similar way, in the secondary resonance MFM, the excitation of the probe is bimodal: the information from the first eigenmode (mechanically excited) is used to obtain the topography but the higher eigenmode is excited by an external magnetic field instead of the piezo. The long-range magnetic forces are separated from short-range allowing a single-pass imaging of topography and magnetic images with high sensitivity, which makes this bimodal MFM technique a useful tool for the characterization of samples with weak magnetic properties.¹⁰⁵ 

Another powerful tool for probing physical phenomena in an MFM-related technique is the study of energy dissipation. In SPM, the dissipation of energy is evaluated by measuring variations in the cantilever oscillation.¹⁰⁶ For MFM, the pioneering work¹⁰⁷ uses these dissipative maps to distinguish between Néel and Bloch DWs or identify pinning sites. It has since been demonstrated that some instrumental artifacts can affect the measured values.¹⁰⁸ 

Classical magnetic dissipation force microscopy (MDFM) studies probe-induced magnetization changes in the sample, but recently the opposite effects have also been studied: the strong probe–sample interaction where the stray field from the sample induces changes in the magnetic state of the probe [Fig. 3(a)]. Iglesias-Freire et al.¹⁰⁹ demonstrated that the magnetic switching at the apex of an MFM probe can produce artifacts in MFM images and could be misinterpreted as a true signal. The authors used this effect to obtain a 3D map of the sample stray field [Fig. 3(b)]. More recently, Jaafar et al.¹¹⁰ discussed a counterintuitive dependence of energy dissipation on probe–sample distance for domains with magnetic moments parallel to the probe's magnetization. Thus, for a large range of distances, the probe–sample separation is directly proportional to the probe's oscillatory excitation energy. The recorded dissipation values (∼fW) were in good agreement with micromagnetic calculations, corresponding to the power losses caused by relatively small regions of spins switching their magnetization. A high spatial resolution (<8 nm) was achieved in the MDFM images; thus, MDFM has been demonstrated to be a promising technique for MNP characterization.^109,111

As MDFM requires a strong probe–sample interaction, which can be a limitation when measuring in high vacuum, Zhao et al.³¹ developed a frequency-modulated capacitive-distance control method, which is valid even in the presence of energy dissipative processes. Another proposed approach for mapping energy dissipation is using drive amplitude modulation AFM (DAM-AFM).¹¹² The method uses the monotonicity of the dissipation force between the probe and the sample to obtain stable images in all environments (e.g., vacuum or liquid suspension).²⁴ In DAM-AFM, the topography map is obtained by using the dissipation of energy as the feedback parameter while the frequency shift, controlled by the phase-locked loop, provides information about the conservative interactions.

Magnetic scanning gate microscopy (mSGM), also known as magnetoresistive sensitivity mapping, modifies the electrical properties of a device under applied voltage in proximity of the scanning MFM probe due to magnetoresistive effects (Fig. 4). From the applied potential difference across the device, electrostatic interactions between the probe and the sample heavily influence the acquired data. Thus, mSGM is often combined with KPFM similar to the KPFM/MFM technique.

The modulated potential difference, induced by magnetoresistive effects from the probe–sample interaction, can be mapped by locking into the frequency of the MFM probe's oscillation across the device with a lock-in amplifier. Hence, the noise generated by the frequencies of the sidebands (from bias modulation on the probe) or the scan rate of the probe across the sample can be significantly suppressed allowing for faster data-acquisition and greater SNR.

In the past, mSGM has been used to characterize giant magnetoresistance (GMR) sensors and obtain sensitivity maps to external magnetic fields. In particular, it has been applied extensively to characterize hard disk drive reading heads.^113,114 Recently, it has been used for the characterization of L-shape permalloy (Py) devices^115,116 and measure the probe stray field using graphene Hall sensors.^117–119 For the former example, devices with pinned DWs were scanned using non-magnetic probes modified with a magnetic bead.¹¹⁶ By monitoring the resistance across the device, it is possible to estimate its sensing volume toward a specific magnetic bead (or any other nanostructure on the probe). This approach enables testing many devices with the same magnetic bead and thus allows correlating results for the optimization of the sensing elements. Other recent developments include using the probe's stray field to manipulate DWs; measuring electrical signals originating from the anomalous Nernst and Hall effects as a way of sensing the position of the DW inside of the nanostructure;^120,121 and writing magnetic landscapes with thermal assistance for magnonic devices.¹²² 

Custom-made MFM probes have been developed to improve the lateral resolution and sensitivity beyond the limit of commercial MFM probes and also to facilitate quantitative MFM (qMFM) studies, e.g., by increasing/reducing the coercive field or modifying the stray field distribution and intensity (Fig. 5). Three trends can be primarily identified: (i) customized magnetic coatings, where the magnetic properties of the material are varied; (ii) probes with magnetic adhered structures, such as Fe-filled carbon nanotubes (CNTs) or magnetic beads; and (iii) MFM probes with fabricated nanostructures.

Among these three approaches, modifying the magnetic coatings of an MFM probe is the most common as it does not require extensive nanofabrication capabilities.^119,123,124 Such probes are characterized by the enhancement of the lateral resolution both in the topography and in the phase/frequency shift signal. This has been achieved, for instance, by partially coating MFM probes,^123,125 or depositing multiple layers of magnetic material to be able to control high/low moment states and if necessary limit the eminent stray-field to the probe's apex.^119,126 The other advantage of customized magnetic coating is a possibility to match the magnetic properties of the probe to that of the sample. For example, reducing the stray field produced by the probe reduces its interaction with soft samples, or conversely increasing the coercivity of the probe helps to image samples with strong stray fields.

A different approach is to adhere magnetic structures to the apex of a non-magnetic probe, which was in many cases used in attempt to create a dipole-like^{116,127–129} or monopole-like probe.¹³⁰ CNTs filled or coated with magnetic material have been attached to the apex of standard AFM/MFM probes^131,132 to improve the lateral resolution of the MFM [Fig. 5(a)]. However, this approach is usually at the cost of the sensitivity, due to the small amount of magnetic material interacting with the sample. Another alternative is attaching microscopic pieces of hard magnetic material¹³³ or magnetic beads^{116,127–129,134,135} [Figs. 5(b) and 5(c), respectively] to the probe apex. In both cases, the typical size (∼1 μm) is far larger than a probe's apex diameter (∼30 nm), which could jeopardize the lateral resolution.

The ability to fabricate nanostructures on the probe apex not only opens the possibility for engineering the magnetic properties by selecting the coating material, but also to use shape anisotropy as a way of governing magnetization. The most common approach consists of using electrical current to induce chemical deposition and hence achieve a sharp apex.^136,137 However, the sharpness of the apex may vary from probe to probe. Another approach uses focused ion beam (FIB) milling to sharpen probes into a needle with magnetic coating only at the very end of the needle^25,138 [Fig. 5(d)]. This approach has the advantage of producing sharp probes with high lateral resolution, but with small magnetic moment. The last type of custom-made probes consists of nanostructures built at the probe's apex to use shape anisotropy to constrain the magnetization and produce a strong stray field just. For example, a V-shaped magnetic nanostructure fabricated on one face of a non-magnetic probe was recently demonstrated [Fig. 5(e)]. Such probes combine a low moment with high coercivity to reduce magnetic switching in the presence of strong stray fields.³⁶ A very recent work¹³⁹ combines all three strategies by first developing a hard magnetic thin film architecture most suitable for MFM on an appropriate flat substrate, creating a nanostructure (slim triangular needle) from the substrate film compound by FIB, and adhering this nanostructure to a non-magnetic cantilever. In the above work, a high resolution MFM probe with unrivaled coercivity and thus stability against large magnetic fields has been fabricated from a SmCo₅ film grown epitaxially on MgO.

MFM probe characterization is a fundamental part of the MFM experiments and particularly relevant for qMFM and in-field MFM. When assessing the suitability of an MFM probe for an application, it is recommended to consider its geometry (e.g., by SEM);^119,121,140 its coercive field (e.g., from in-field MFM);^131,141 and its magnetization profile (e.g., by electron holography,^119,121,140 measurement of a reference material,^36,130,142 or Hall sensors^{118,119,143–145}).

This disregards an important aspect of the magnetostatic interaction: the interaction volume of a realistic 3D probe with the stray field of the sample $H s →$ will depend on the size and morphology of the measured domains. Thus, PPA models should be applied only to samples with regular stray field patterns. Recent works report on the moment quantification in laterally confined objects such as MNPs,^93,147–149 chains of magnetosomes,¹⁵⁰ or patches of single molecular magnets (SMM).¹⁵¹ In most cases, the tip's point-probe parameters were freely adjusted to allow a self-consistent data description, but not determined from an independent sample. On the other hand, quantifying the signal of an individual nanoscale object is not easily done with other methods. Uhlig et al.¹²⁷ made use of the point probe character of an MNP by picking up such a particle with a non-magnetic probe. By preparing such an MFM probe, the PPA model description is more justified than for volume probes.

The TTF approach by Hug et al.²⁵ calculates the force on an MFM probe exerted by the stray field of a sample with perpendicular magnetization in Fourier space (Fig. 6). By means of a calibration measurement of a suitable reference sample, one derives a model-independent and parameter-free description of the probe's imaging properties. Considering that even nominally identical probes (from the same manufacturer/batch) can result in large variance of the MFM contrast on an identical sample, this experimentally more elaborate approach is thus judged to be of great importance. The researchers have successfully applied this approach to experimental means, e.g., for the quantification of non-compensated moments in exchange-bias systems.¹⁵² Neu et al. have followed this qMFM approach for, e.g., identifying the vortex state in a magnetic nanowire,⁵⁴ calibrating custom-made probes,¹¹⁹ or quantifying the stray field in the corner of an L-shaped Py structure.³⁶ A recent application of qMFM quantifies artificially patterned stray field landscapes in CoFe/MnIr exchange bias layer systems.¹⁵³ Although successful, this study also reveals the difficulties that arise with the quantification of a complex multiscale domain pattern. Reference samples and measurements need to cover a large range of spatial frequencies to correctly calibrate the probe for all relevant length scales. Due to the even larger complexity and multi-scale character of magnetic domains present in modern permanent magnets,¹⁵⁴ qMFM measurements have not yet been performed on this important set of materials, although it is expected that highly resolving and quantitative MFM measurements can lead to a large improvement of their understanding.

In this part, we discuss applications of MFM and the relevant daughter techniques to modern areas of the physics and the material science. It is noteworthy that such applications are very often quite challenging, dealing with extremely low magnetic signals and requires the ability to distinguish the magnetic response from the other components (i.e., electrostatic contributions, magnetic contaminations, etc.).

We start this part of the Perspective article from considering applications of MFM to thin films with PMA. The interpretation of MFM images is most straightforward for samples with a magnetization orientation perpendicular to the imaged surface. Here, the stray fields produce a qualitative MFM contrast that closely resembles the underlying domain structure. Hence, a wealth of MFM studies focus on the imaging and interpretation of films with PMA, quantified by the (perpendicular) uniaxial anisotropy constant K_u. In the case of large PMA (ideally $K u≫ K d= 1 / 2 μ 0 M s 2$⁠), these band domains can be approximated as being homogeneously magnetized along the z-axis (colloquially up or down), forming a domain morphology that depends among others on field history, surface corrugation, and coercivity. Domain theory of such high PMA films is well established and the knowledge of M_s, K_u and the DW profile allows a correct quantitative reconstruction of the magnetic domain pattern (or, equivalently, the effective magnetic surface charge pattern) from a qualitative image. Thus, such films are well-suited as reference samples for probe calibration (see Sec. III B).

Recent MFM work on films with PMA can be roughly segregated into the following four groups. The first group deals with films with large PMA, where the equilibrium domain width can be used to judge the balance between the various energy terms. For thin film systems with DMI, such comparisons between domain theory and observed domain widths gathered great importance to conclude on the less accessible DW energy.¹⁵⁵ 

The second group considers films with smaller PMA (i.e., K_u < K_d), where the dominating shape anisotropy pulls the magnetization vector into the film plane, but still the presence of PMA can lead to a modulation of the magnetization vector perpendicular to the surface. These stripe domains are again observable by MFM but the magnetization possesses a complex depth dependent structure, which can only be approximated by analytical theory and otherwise requires micromagnetic calculations. A recent work demonstrates the influence of the weak PMA on the domain structure in soft magnetic NdCo₅ films with anti-dot structure.¹⁵⁶ Evaluating stripe domain patterns in a quantitative way has so far not been accomplished to satisfaction. This is due to the lack of qMFM studies and the difficulties in theoretically describing the magnetization pattern.

The third group includes samples, in which a layer with PMA is exchange-coupled to a soft layer with in-plane magnetization, which are a subject of recent studies to obtain a microscopic view of how exchange-coupling occurs in layers with orthogonal anisotropies, see, e.g., an example on the [Co/Pd]/Py system in Ref. 157. The final group includes laser-induced manipulation of a sample's magnetization state, which can be imaged with high resolution by MFM and may give insight into the origin of loss and sometimes also restoration of magnetic order.¹⁵⁸ 

Beyond thin-films, another highly researched topic of study is patterned magnetic media. Patterning FM materials into novel shapes and structures is of particular interest in applications such as logic devices or novel magnetic recording.¹⁵⁹ As methods for patterning materials on the nanoscale improve, as they have been consistently, ways to characterize the new synthetic designs are required to measure the exotic and useful properties they possess. MFM previously has been highlighted as an important tool for understanding the magnetism within such structures, ranging from memory devices (e.g., bit-patterned media)¹⁵⁹ to magnetic strips, and nanodot and antidot arrays.^160–163 One of many modern examples of magnetic patterned structures that are popularly researched are artificial spin ice (ASI), which exhibit geometric frustration, ordering of effective magnetic charges, and a variety of collective dynamics.^164–166 

ASI consists of lithographically patterned arrays of nanoislands/NWs of different designs composed of in-plane FM material, which are magnetically frustrated due to the intrinsic geometric ordering to create two out-of-plane Ising-spins for each nanoisland.^167–169 ASI have received attention as the frustrated arrays can be controllably pinned into multiple stable/meta-stable states, priming them for magnetic recording, logic devices, and experimental hot-beds for understanding magnetic frustration in more complex systems. In their ground-state, some of the most popular structures in literature {e.g., squares and honeycomb lattices [Figs. 7(a)–7(d), respectively]} obey the ice-rule,¹⁶⁷ but can be excited into higher energy states by external stimuli (e.g., by applied field). Wang et al.¹⁷⁰ demonstrated reading, writing, and erasing of individual bits by applying in-plane field below the nanoisland saturation-field and individually switching nanoislands with an MFM probe, demonstrating great precision for single bit writing. Gartside et al.¹⁷¹ similarly introduced topological defect-driven magnetic writing on ASI using the MFM.

Another extremely interesting example of recent objects of MFM studies are magnetic topological structures. Topological solitons, or defects, in magnetic materials have provided, and continue to provide, a rich plethora of phenomena to be studied for fundamental research^66,172–174 and future magnetic based technologies,¹⁷⁵ which rely on various novel magnetic configurations and architectures. Typically, these defects in magnetic materials are manifested as magnetic domain-walls,^176,177 vortices,^178–183 skyrmions,^184,185 or magnetic bubbles.¹⁸⁶ Here, we focus solely on the use of MFM in observing and quantifying physical phenomena occurring in DWs and vortices. MFM studies of skyrmions will be discussed in the Perspective section (Sec. IV C).

Magnetic domains and the walls that divide them are determined by the subtle balance of the following main contributions of micromagnetic energy: exchange interaction, magnetostatic, and magnetocrystalline energies.¹⁷⁷ Understanding DW motion and dynamics under the influence of an applied stimulus such as magnetic field or spin polarized current pulses can elucidate to the complex underlying magnetization reversal processes and how DWs can be manipulated for use in modern technologies. Here, MFM excels as a tool to investigate phenomena such as the domain structure in magnetic nano-patterned elements following the application of an external stimulus in so-called quasi-static measurements. This is of high importance for technological applications of DWs¹⁸⁷ such as that of the racetrack memory (RM).¹⁷⁵ RM offers a significant gain over conventional magnetic storage devices and potential silicon based logic circuitry in terms of performance.¹⁸⁸ Here, spin polarized currents are used to generate spin torque transfer^189,190 such that DWs in the racetrack can be moved along a track, which extends into the third dimension^175,191 increasing the effective bit density. Recent developments have moved to more exotic phenomena to drive domain-wall motion where spin-orbit torque (SOT) effects, such as the Rashba effect^192,193 and the spin Hall effect.^194,195

MFM is also often used to investigate complex domain type structures where the geometry, hence the magnetostatic energy, of the material system starts to play a dominant role.^196–198 This alters the equilibrium configuration such that it becomes more complex than in the typical cases of Bloch or Néel type DWs in thin films. Examples of these wall types include the transverse/asymmetric transverse DWs^196,197,199 and single, as well as multiple, vortex walls.^196,197,200 Understanding the internal structure of such DW configurations is important not only for scientific interest but also for applications as the internal structure strongly dictates the DW dynamics.¹⁷⁴ Recently, Nguyen et al. have demonstrated that in Py nanostrips with in plane magnetization a so-called Landau DW exists.¹⁷⁴ This novel DW configuration is described as a flux closure pattern that resembles a Landau pattern; however, it is elongated and encircles a Bloch type wall. In this work, MFM was integral in confirming the predicted domain configurations obtained by employing finite difference methods to solve the Landau–Lifshitz–Gilbert equation.²⁰¹ 

Of particular interest is the case of cylindrical wire and FM nanotube type geometries. Arrays of such wires have potential in many advance technological areas, including data storage and information, energy, Life Science, and environmental sectors.²⁰² Furthermore, numerical simulations have predicted that the Walker breakdown limit in such 1D nanostructures is topologically forbidden,²⁰³ making them extremely attractive for technological applications requiring DW displacement. In these geometries, a number of topological defects can be identified: transverse DWs; asymmetric transverse DWs; and Bloch point walls, which are similar in nature to vortex walls found in FM nanotubes.²⁰⁴ Due to its high spatial resolution and sensitivity, MFM has been widely used to study the domain configurations of such wires. For example, it has been shown that in Co NWs of dimensions 45 nm in diameter and 10 μm in length, an alternating pattern of vortex states is energetically favorable, offering an interesting route toward novel spintronic memory devices.²⁰⁵ Similarly, the spin configuration has also been investigated in Co bamboo-like NWs with diameter modulation.²⁰² Using MFM, it was demonstrated that, due to the competition between the magnetocrystalline and shape anisotropy energies, multi-vortex structures with alternating chirality form. Interestingly, as it was evidenced by MFM data, DW pinning in the modulated diameters wires was avoided, in contrast to other materials (FeCuCo) investigated in the same study.

In addition to DWs in thin films and nanowire type topographies, confinement in nano-sized patterned elements can lead to stabilization of vortex cores.¹⁷⁹ These are of particular interest due to the potential they hold for future microwave sources magnetic sensors and logic as well as in non-volatile memory applications.²⁰⁶ Vortex cores exist as a thermally stable flux domain pattern that can be typically characterized by in plane winding of the magnetization around a perpendicularly magnetized core.¹⁸² Vortex cores, which can be as small as 10 nm¹⁸¹ in size, possess polarity ±p with respect the out-of-the-plane axis and a given chirality. MFM has proved to be an invaluable tool for the investigation of vortex cores and indeed was used in the first observations of vortex cores in patterned disks of Py.¹⁷⁸ Additionally, MFM has been utilized to explore the switching of vortex cores using current driven vortex excitation via spin torque transfer¹⁸⁰ paving the way for electrical control of magnetization in logic devices.

Multiferroics are another modern class of material where MFM and MeFM are used, often in conjunction with piezo force microscopy (PFM) studies. The coupling between the magnetic and electric dipoles in multiferroics holds vast promise for conceptually novel electronic devices and has been widely explored in the last decade. ME phenomena have a profound and broad impact on diverse areas of materials science from multiferroic materials to topological insulators, where direct visualization of ME domains and DWs is of both fundamental and practical importance. Specifically, MFM has been proven as an essential technique for studies of multiferroic (in particular, ME) materials that exploit both FM and ferroelectric (FE) properties.

MFM is typically used to reveal the microstructure of both single-phased multiferroics and multiferroic composites, such as detection of the strong magnetic contrast, visualization of the magnetic structure of grain boundaries, and reviewing the appearance of non-magnetic pores between the phases in nanostructured ME materials.²⁰⁷ MFM imaging was used to reveal the presence of magnetic domains being extended over several adjacent ferrite grains in BaTiO₃(Ni_0.5Zn_0.5)Fe₂O₄ multiferroics²⁰⁸ and in BiFeO₃ NWs.²⁰⁹ In many cases, it was advantageous to use extended modes of MFM, i.e., in-field MFM or under the action of electrical poling.

Additionally, MFM was used to establish the nature and overall contribution of the material properties originating from magnetic and multiferroic defects. In the relaxor FE single-phase (BiFe_0.9Co_0.1 O₃)_0.4 – (Bi _1/2K_1/2TiO₃)_0.6, CoFe₂O₄ magnetic clusters with sizes 0.5–1.5 μm were identified using MFM.²¹⁰ Such inclusions exhibit solely a magnetic dipolar response, indicating magnetization along the in-plane orientation. On the other hand, a combination of MFM and PFM showed that multiferroic clusters (unspecified in nature) exhibit both FE and strong magnetic properties. It is expected that these findings will lead to new research in this novel class of non-ergodic relaxor multiferroics, especially as the material is Pb-free and consists only of abundant elements.²¹⁰ The overall concept is ideal for an electrically controlled magnetic nanodot storage device.²¹¹ 

Local MFM studies were used to directly demonstrate magnetization reversal under purely electrical control in another BaTiO₃/Ni system, which is the overall goal in magnetoelectrics.²¹² The authors primarily used MFM to study a commercially manufactured multilayer capacitor that displays strain-mediated coupling between magnetostrictive Ni electrodes and piezoelectric BaTiO₃-based dielectric layers. The authors evidenced that the anisotropy field responsible for the perpendicular magnetization could repeatedly be reversed by the electrically-driven magnetic switching. The demonstration of non-volatile magnetic switching via volatile FE switching was used to inspire the design of fatigue-free devices for electric-write magnetic-read data storage.²¹² 

Direct visualization of ME domains in multiferroics was demonstrated using low temperature in situ MeFM from lock-in detection of electrically-induced magnetization. The authors directly demonstrated the local intrinsic ME response of multiferroic domains in hexagonal ErMnO₃⁹⁵ and YbMnO₃,⁹⁶ distinguishing contribution of six degenerate states of the crystal lattice, which are locked to both FE and magnetic DWs. Results were in excellent agreement with the symmetry analysis, and a giant enhancement of the ME response was observed in proximity of the critical temperature. This suggests that critical fluctuations of competing orders may be harnessed for colossal electrically-induced magnetic responses (Fig. 8). The use of cryogenic in-field MFM was also demonstrated by Wang et al.⁹⁸ Labyrinth-like domains (∼1.8 μm) in an h-LuFeO₃ thin film were observed after zero-field cooling below the Néel temperature, T_N ≈ 147 K, where weak FM order with a canted moment exists. At 6 K, MFM images of the magnetization reversal process reveal a typical domain behavior of a pinning-dominated hard magnet. The temperature dependence of the domain contrast demonstrates that MFM is able to detect the domain contrast of magnets with miniscule magnetic moments (∼0.002 μ_B/f.u.).

Moving away from traditional applications in physics and material science, MFM has lately gained a momentum for studies of magnetic nanomaterials for Life Science applications. There are a broad range of applications using magnetic beads and MNPs, including cell separation, bio-sensing, in vivo imaging, magneto-thermal therapy, etc.^213,214 Alternatively, the use of elongated nanostructures such as magnetic cylindrical NWs is of growing interest in different bio-magnetics applications due to their high aspect ratio, anisotropic physical properties, and the possibility to work with different length scales.²¹⁵ 

A direct characterization of the magnetic properties of individual beads and MNPs on nanoscale is possible by microscopy-based techniques such as MFM. For example, MFM has recently been used to detect superparamagnetic and low-coercivity^{79,80,148,216,217} MNPs. Also, MFM has been successfully employed to characterize MNPs inside biological systems as vesicles (niosomes),²¹⁸ virus-capsids,²¹⁹ or cells,²²⁰ where MFM images were used to evaluate the amount of magnetic material inside the different entities.

In addition, MFM has been used to investigate the magnetic properties of individual NWs²²¹ and vortex-state dots¹⁶³ for biomedical applications. Molecules, such as ferritin, have also been characterized by liquid-MFM.^222,223 However, it is crucial to remember that MFM is sensitive to non-magnetic (e.g., electrostatic) signals (see Sec. III A),⁴⁴ especially in the case of biological systems, where the strength of the produced stray field are near the sensitivity limit of the technique.

In the case of biological systems, it is essential to perform sample characterization in relevant environmental conditions, e.g., physiological environment. For that reason, non-standard methods such as bimodal,¹⁰¹ energy dissipation,¹¹¹ or AC-field modulated MFM^224,225 have been explored in recent years. Another approach is the use of custom magnetic probes specifically designed for biological applications.^123,226 However, since MFM has historically been applied to the study of inorganic materials, the potential of MFM for biological/biomedical applications is still under development.²²⁷ Recent studies have demonstrated essential MFM capabilities (i.e., high enough lateral resolution and sensitivity) for studies of individual MNPs in a liquid environment.²⁴ This development opens new possibilities of studying magnetic systems in biologically relevant conditions.

The Perspective part of the paper presents the emerging trends in the field of MFM concerning further development of instrumentation (e.g., in combination with other SPM modes and radiation techniques), the wider applications of qMFM measurements, and application of MFM and its sister modes to studies of advanced and emerging materials.

The MFM community incorporates a variety of users: from beginners that demand a friendly and reliable interface to the highly specialized researchers that customize or even build their own system. It should not be forgotten that the majority of the commercial MFM users are interested in pushing the resolution and sensitivity limits of the technique. While commercial, off-the-shelf systems still remain a valid indispensable tool for a routine inspection of magnetic properties of samples, modern challenges in both research and industry demand development of new advanced MFM modes. To fulfill this need, the current research is targeted in different directions, including the development of a new MFM instrumentation and flexible software, novel types of MFM probes (a key point still under development), and combination of MFM with other techniques targeting complex material properties, which is a general trend to make the MFM compatible with the simultaneous transport, thermal, or optical characterization. Finally, there are several groups that push the MFM technique to the limits of high speed scanning, fast signal processing, and recording than allow exploring high frequency processes.

Often the realistic experimental needs require measurements in a specific, precisely defined environment, e.g., temperature (i.e., low, high, or variable), pressure, humidity, specific gas atmosphere, vector magnetic field. Typically all these options are not available commercially but rather custom-developed as a research tool (see Sec. III A). Another rapidly filling niche is the development of custom-made magnetic probes. While commercial suppliers usually offer magnetic probes of three main types (i.e., standard, low-/high-moment), the customized options provide a significantly larger variety of probes with properties targeted to a specific (sometimes very narrow) application. The examples include the probes functionalized with magnetic nanoparticles and microsized beads, Fe-filled CNTs, one-side coated switchable probes, lithographically modified probes (e.g., Fig. 5), etc. Another important option is an ability to separate magnetic and electrostatic signals and successfully eliminate the latter. This is an imperative option for samples with low conductivity or electrically biased devices.^{118,119,145,228} One of the most promising trends is the development of new multifunctional systems, allowing combined mapping of magnetic and additional functional properties (e.g., KPFM-MFM, MF-PFM, MF-SEM, etc.), or measurements of magnetic properties by different means [e.g., in tandem MFM and magneto-optical Kerr effect microscopy (MFM-MOKE)]. All these combined modes are currently available only as custom-developed options (often due to a limited size of the specialized market). However, it might be expected that they will soon find their way to commercial options.

For the latter example, the combination of a MOKE microscope and MFM provides an interesting and powerful tool to study novel magnetic materials,²²⁹ not only at different length or time scales, dictated by the two methods, but it also allows for flexibility in terms of the magnetic sample to be investigated in a highly efficient manner. For example, by utilizing the MOKE, one can tune the domain pattern or magnetization state of the sample and image within the, diffraction limited, resolution of the microscope. Then, subsequent MFM investigations can follow which would allow for higher spatially resolved images to be taken. This combination has been utilized to image the domain structure of NbFeB crystals.²³⁰ In this work, two data analysis techniques were used to gain further insight to the magnetic structure, including surface charge pattern and local susceptibility. This is achieved by taking the difference and sum images, respectively, of two subsequent scans with oppositely magnetized probes. This allows the general domain structure through charge contrast images and also the variation in the sample permeability through the susceptibility contrast images to be obtained. Due to the depth sensitivity of the two techniques, complimentary information of the surface as well as the general magnetization structure within the domain can be investigated. Such functionality has significant merits for topics that are currently investigated and feature in this Perspective section. For instance, systems hosting skyrmions or bubble domains could be studied using this combined approach. Due to the different skyrmion sizes possible, a cross-over between the two techniques would be defined: Kerr effect for a general overview and location of optically resolvable features and MFM, which would be used as more local probe to investigate the stray field signatures of the skyrmions. This is particularly interesting in terms of the field protocol used to nucleate and annihilate skyrmions as it would allow for a broad understanding of the regions of most interest in a timely fashion rather than searching within the field-of-view of the SPM. Although the original combination of MFM and MOKE was published relatively long time ago,²²⁹ there is now a clear industrial interest from the companies in resuming this type of instrumentation on a commercial scale.

Multi-functional microscopes, with the ability to combine data from different sources into a single image as well as controllably and reproducibly modify the sample's state, are becoming more and more ubiquitous. For instance, quite recently, it has become possible to use an AFM inside of an SEM chamber to combine the two imaging techniques^231,232 or to perform nanofabrication with the focused-ion beam (FIB).²³³ This new instrument, called AF-SEM, works in vacuum conditions and allows for large scanning areas and positioning the probe in ways that are typically non-accessible to normal MFM. This is of interest when considering the possible shift from 2D fabrication towards 3D magnetic nanostructures, since AF-SEM will enable navigating complex samples and perform MFM on different faces of a 3D structure.²⁰⁵ 

Another interesting system, in particular for in-liquid MFM,²³⁴ is a combination of a SPM and an optical microscope where the latter includes functionalities such as confocal- or fluorescence microscopy. The combination of MFM with these techniques will further enable a range of Life Science studies (e.g., related to MNPs applications or combined magnetic and optical labeling).¹⁵⁰ For example, in a typical experiment where the cells are sensitive to light, proteins marked both with MNPs and fluorescent markers are introduced to the extracellular medium. Using either fluorescent or confocal microscopy, it is possible to study the large scale distribution and see if the MNPs are internalized by the cells, while using the MFM, it is possible to detect individual MNPs and characterize their distribution at the nanoscale level inside of the cell without having to expose the cell to high intensity light.^235,236

Apart from combining different imaging techniques, the possibility of performing manipulation or modifications on the samples under study during imaging is a growing trend that has seen big developments in the last couple of years. For example, some SPM systems now include a lithography mode where the probe follows a custom-defined path, while a deflection or bias voltage are applied to the probe.^122,171,237 Such experiments have been performed to move/capture magnetic beads, to induce defects/nucleation sites in magnetic films, and more recently, to print 3D nanostructures. The possibility of inducing defects/nucleation sites in magnetic films and nanostructures has a wide range of applications, since the lithography mode allows direct introduction of desirable magnetic sites, while MFM enables imaging the magnetization distribution and its consequent evolution. Additionally, the possibility to manipulate magnetic beads using MFM enables single magnetic bead studies. Finally, the 3D-printer AFM, which operates in-liquid and uses a hollow probe to deposit materials,²³⁸ is a system that has so far demonstrated rapid nanofabrication capabilities, without the need of a clean-room or e-beam lithography, which are expensive facilities that limit the access to nanofabrication. This is an excellent system to be combined with MFM, since the 3D printer enables building magnetic nanostructures, and the MFM allows imaging them to check if the magnetic nanostructure behaves as expected.

The ability to perform real-time MFM is a desired function for researchers in micro-/nano-magnetics as it would combine operational simplicity and availability of an SPM system in non-specialized environment with the power to map real (quasi-)dynamic effects, rather than “freeze-frames.” Such advancements would allow for the study of effects such as domain wall propagation/creep or magnetization relaxation in MNPs with relative ease and high resolution. High-speed AFM (HS-AFM) is not a new concept, with commercial systems capable to map topography with sub-100 ms time-resolution rather than minutes in standard AFM,²³⁴ but speeds required to study a multitude of dynamic magnetic effects (potentially μs resolution and below) appear to be unlikely within the current scope of developments (as of time of writing) for MFM.

Another interesting concept in MFM-instrumentation is the ability to perform volumetric magnetic measurements. Until recently, SPM was traditionally performed in zero- (e.g., noise measurements), one- (e.g., variably-field MFM), or most typically two spatial dimensions in the sense that a change in physical interaction between the probe and the sample is quantified within a defined Cartesian coordinate above the basal plane of the surface. More advanced SPM techniques have extended into the third dimension by mapping chemical/physical properties with respect to physical matter interactions (e.g., vibrational modes in chemical bonds by tip/surface-enhanced Raman²³⁹ and scanning nano-IR microscopy²⁴⁰) or xyz (i.e., volumetric) data-acquisition (e.g., AFM force volume measurements^241,242). Volumetric MFM can be performed on commercial instruments as it is largely based on the force volume methodology; the fast axis in such measurements is the z-axis, thus the probe generates force curves at each xy-pixel coordinate, mapping the phase change as a function of z-displacement (Fig. 9). Despite this, MFM has instead largely stayed within a single spatial plane, despite the recent scientific drive towards “big data” in other areas.²⁴³ The likely causes for this shortfall thus far is large data-sizes and lengthy acquisition times.

3D-data for MFM does not exclusively refer to the three spatial dimensions, and there are many examples where the 3rd variable is an alternative controllable property such as temperature or applied field, which have been discussed throughout the Review section. A recent example of acquiring 3D matrices of MFM data is provided by the demonstration of the general-mode (G-Mode) SPM system.²⁴³ This system samples the entire photodetector response of an SPM with a MHz sampling rate, generating a three-dimensional datasets (after post-segmentation). One interesting application for G-Mode is the identification and separation of magnetic and electrostatic interactions in MFM.²⁴⁴ 

However, volumetric MFM datasets remain uncommon, despite improved data acquisition (in part developed from the popularity of functions such as force-volume) and more available tools/software for 3D-data visualization and analysis.^245–247 Volumetric MFM is largely an under-researched area in which, with further development of data-handling practices, statistics, and with utilization of modern techniques such as machine learning, interesting properties could be quantified at the nanoscale without specialized/expensive equipment, e.g., 3D calibrated characterization of MNP's stray field, magnetization dynamics with respect to perturbing fields, or probe calibration/characterization.

The development of new MFM instrumentation goes hand-in-hand with the development of new magnetic probes built on demand (both custom-made and commercial). Customized probes are used to perform very specific tasks and push the limits of commercial MFM systems, e.g., to achieve a higher resolution; reduce/increase probe–sample interaction; or to be able to combine different scanning modes. An example of the latter is the use of a probe that is both magnetic and conductive simultaneously, enabling the instantaneous extraction of both magnetic and electrical signals.⁵ 

Due to targeted specificity and high production costs, the market for customized probes is often small; thus, many of the proposed modifications do not become available as commercial products. However, occasionally some of the new designs become commercially valid due to a reduction in fabrication costs and growth of the market for MFM (and other SPM techniques).¹²³ The MFM probe with partial coating²⁴⁸ is an example of this, where only one side of the probe's tip is coated with magnetic material; this reduces the magnetic moment of the probe, achieving a higher spatial sensitivity. The magnetic coating of this probe model is deposited in such a way as to prevent also coating the cantilever with magnetic material, reducing the cantilever-sample interaction. Another example of custom probes entering the market is the MFM probe where the magnetic element is either inside or at the end of a CNT attached to the probe's apex. These probes are suitable for commercialization due to their apparent advantages (very low magnetic moment, high spatial resolution, and extremely low probe–sample interaction), which are becoming more and more critical in growing fields such as bio-magnetism or magnetic topological structures (e.g., skyrmions).

An emerging technology is the multifunctional nanoscale sensor, which is able to detect several types of interaction simultaneously, rather than being used only for a single application. Examples of this include the use of magnetic probes in near-field systems such as scanning near-field microscopy²⁴⁹ or tip-enhanced Raman spectroscopy.²⁵⁰ This multifunctional approach would allow the production of probes to be more cost-effective. For example, the single application probe used in scanning thermal microscopy has very costly fabrication steps, but the addition of a magnetic coating, to create a multi-functional probe, would add only a relatively small cost to the total amount. In addition to the economic advantage, multifunctional probes are able to significantly reduce imaging time as they are able to simultaneously extract several different data types. It could be argued that the data quality would also be increased as the necessity to locate the area of interest with a multiple probes would be eliminated.

Despite the limitations faced in making customized probe models widely available, there are several examples of new probe models being adopted by MFM probe suppliers. While the probes with single functionality are expected to continue to dominate the MFM market in the short term, the multifunctional approach is expected to see greater success in the medium to long term, due to increased cost-effectiveness and added probe functionalities that are advantageous to users.

Typically, macroscopic magnetic field measurements are traceable to nuclear magnetic resonance quantum standards and traceability chains to industry are already well established. However, these calibration chains only relate to measurements of fields that are constant and homogeneous over macroscopic volumes or surface areas down to the millimeter scale. At the same time, key international high-tech industries such as magnetic sensor manufacturing, precision position control and sensing in information technology, consumable electronics, and Life Science, as well as in R&D require traceable and reliable measurements of magnetic fields and flux densities on the micro- or nanometer scale, e.g., for quantitative analysis and quality control. In order to address the gap between the technological capabilities and the industrial needs, a collaborative European metrological project (NanoMag; http://www.ptb.de/empir/nanomag.html) has been established. The overall goal of this project is to develop and provide coordinated and sustainable European metrology capabilities that extend reliable and traceable measurements of spatially resolved magnetic fields down to the micrometer and nanometer length scale. Development of the standards for traceable calibrations for MFM is one of the primarily goals of this project. The prime outcome of the project is related to the development, comparison, and validation of calibration procedures for traceable quantitative MFM measurements as well as establishing a high level of metrological MFM capabilities across Europe.

Quantitative stray field measurements on the sub-50 nm length scale, which can be easily achieved by qMFM, have a multitude of applications. One of the largest is the realization of position control devices, which due to the much-reduced length scale will find use in appliances, automotive, and consumer electronics. Furthermore, tailored magnetic stray field landscapes on the micrometer and nanometer length scale allow controllable magnetic micro-bead and/or nanoparticle manipulation and transport^220,251 in future cost-efficient lab-on-a-chip devices for biological, chemical, medical, and life science applications. Finally, a multitude of scientific studies, which are already tackled by MFM (see Sec. III C), would benefit from a quantitative analysis. We just mention two large fields: (a) isolated nanoscopic object, in which size and magnetic nature are not fully known [e.g., core-shell particles (see Sec. III B) with a non-magnetic oxide shell, structured thin film elements with a magnetic dead layer] and cannot satisfactorily be studied by global magnetometry, but could be quantified microscopically. (b) Reconstructing the magnetization state from stray field data is an ill-posed inversion problem in magnetostatics. This is even more problematic, when inhomogeneous magnetization structures or magnetization textures are to be resolved. While qMFM may not be able to unambiguously reconstruct such textures due to fundamental limitations, it allows to decide between different hypothetical models. Thus, inhomogeneous magnetization states, such as stripe domains in films with weak PMA (Sec. III C) or skyrmions (Sec. IV C) can be identified and distinguished from band domains or bubble domains when MFM measurements are analyzed quantitatively.

With increasing automation of both measurement capabilities and analysis procedures in modern AFM/MFM instrumentation, qMFM based on the most versatile TTF approach will become accessible for routine MFM experiments. The most important requirements are the availability of appropriate reference samples and of dedicated analysis software. Few groups do already have these capabilities^25,54 and they are currently being evaluated and developed further for dissemination to the public in the current European metrology project NanoMag (http://www.ptb.de/empir/nanomag.html), including analysis software tools in the scanning force data analysis package Gwyddion.²⁴⁵ A second requirement is the availability of artifact-free, low noise, and reproducible MFM data, which is aided by the improved stability and ease-of-operation in modern SPM-instrumentation.

Automation of measurement procedures (using scripting and batch processing) will allow repeated measurements with unchanged parameters (for improved signal-to noise), repeated measurements with systematically changing parameters (e.g., varying lift height for confirming the correct decay behavior of stray fields and thus excluding artifacts), and also alternating measurements between reference sample and the sample of interest (to judge the stability or wear of the probe's imaging properties during repeated use). Automation of analysis procedures will easily allow for, e.g., drift corrections, averaging, or more complex mathematical operations (filtering, deconvolution, etc.) of images, which finally results in a quantitative evaluation of the MFM probe or the magnetic sample under study.

We further describe a required standard procedure for calibration. Prior to an automated quantitative measurement of a sample under study (i.e., measurand), MFM probe, reference sample, and measurement procedure have to be properly selected to reveal the desired information. The main characteristic of a reference sample is such that its domain or stray field pattern can be quantitatively constructed from the measured MFM data without detailed knowledge of the yet to be calibrated probe. A reference sample can be a thin film with known PMA and saturation magnetization in a multi-domain state (see Sec. III C), the stray field landscape of patterned thin film elements in a single domain or the stray field of current-carrying wire structures. Most important for the choice of reference sample is that it covers all spatial frequencies present in the studied sample.

A standard procedure for quantitative MFM is envisioned as a flow diagram (Fig. 10). An alternative to the final block (red) is to develop a hypothetical surface charge/stray field model of the sample and construct a theoretical MFM pattern via convolution with the agreed TTF. The model should be modified until sufficient agreement with the experimental MFM pattern is achieved.

We further discuss the application of MFM to studies of advanced and emerging magnetic materials and structures, namely antiferromagnets, spin-caloritronic materials, skyrmions, topological insulators, 2D materials, and van der Waals crystals as well as application of MFM to multidisciplinary life Science and environmental studies, which are often beyond a “traditional” physics approach.

The applicability of MFM to characterize the stray magnetic fields from magnetic recording (MR) and logic devices is historically well established in literature. In earlier studies, Rugar et al.³² reviewed the application of MFM to longitudinal recording media, and ever since there has been numerous studies of MR by MFM along with a host of other techniques. However, the bit capacity for modern MR (e.g., those based on perpendicular MR devices) has accelerated to the point where they are almost beyond the limits of the spatial resolution for standard MFM. As a consequence, MFM is currently a confirmatory technique for characterizing stray fields in MR devices industrially, used in tandem with other imaging methods. Further development in MR is certainly going to continue at pace, potentially circumventing the practicality for MFM imaging devices directly as it will not be able to fully resolve the features. However, MFM's simplicity and availability means that, although it may not be used to characterize MR devices directly as it has been used historically, it may remain a popular tool for research in this area in other ways, as we have seen for heat- or microwave-assisted magnetic recording (HAMR and MAMR, respectively),^89,252,253 which are modern technologies set for commercial markets. Examples of some creative studies into these devices with MFM includes work by Chen et al., who used MFM and MOKE to probe the erasure of the pre-recorded magnetic patterns as a function of laser power,²⁵⁴ and to experimentally study a novel bi-layered HAMR architecture that has one layer for conventional MR and a dedicated servomechanism in the underlayer.²⁵⁵ More novel MR concepts, such as racetrack memory (see Sec. III C) shall remain a significant research topic as MFM offers the ability to image the domains in a quasi-dynamic state, and quality test the imperfections in NWs, which currently limits the DW velocities in devices.

Antiferromagnetic materials are interesting for spintronic applications due to the great variety of inherent phenomena they possess.^256,257 These include absence of stray fields due to fully compensated magnetic moments, resilience to externally applied fields, and faster spin dynamics than those of FM materials due to high magnetic resonance frequencies of the order of THz. These properties make them attractive for applications such as antiferromagnetic-based memory. It has recently been demonstrated that current induced torques can be used to shift the orientation of the Néel vector in CuMnAs,²⁵⁸ resulting in the all electrical reading and writing of antiferromagnetic recording media. Indeed, thin films of Cr₂O₃ have been studied due to their ME effect which can be significantly enhanced when the thickness dimensions are of the order of a few nanometers.²⁵⁹ Here, MeFM has been extremely successful in identifying the antiferromagnetic domains in Cr₂O₃. Furthermore, the antiferromagnetic properties of Cr₂O₃ combined with its ME effect can be used as an active exchange bias layer that can be modified electrically which can manipulate the FM state of exchange coupled magnetic layers.²⁶⁰ 

It is expected that both MFM and MeFM will be adopted on a broader scale in order to understand better the local magnetic properties of antiferromagnetic materials. The intrinsic properties and hence the functionality of such materials are extremely dependent on the local degree of disorder and defects. The information gained by MFM and MeFM will be invaluable for the miniaturization of current antiferromagnetic based spintronic, multiferroic systems,²⁶¹ and understanding of the role defects play in these materials. This is evident in recent investigations of multiferroic hexagonal rare earth manganite where MeFM was used to observe ME domains on a micrometer scale.⁹⁵ Here, it was evidenced, by observing a divergence in the ME effect near the tri-critical point using MeFM, that an enhancement of the ME effect in h-ErMnO₃ could be possible by utilizing critical fluctuations. Combinations of MeFM and MFM at low temperatures are anticipated to play a crucial role in the understanding and further development of multiferroic and antiferromagnetic materials exhibiting ME coupling on the micro- and nanoscale.

Further to the investigation of antiferromagnetic order by MeFM,^35,97 applications of MFM are likely to be employed for studies of defects in antiferromagnetic materials such as NiO. It has been shown that crystallographic defects can exhibit significantly different magnetic behavior to that of the lattice, where MFM was used to visualize dislocations at the individual level.²⁶² Moreover, it was found that it was possible to create such dislocations in order to generate high stability and high coercivity FM elements embedded in an antiferromagnetic environment, where the ferromagnetism arises due to the off-stoichiometry of the dislocations.

Spin caloritronics studies the combination of thermoelectric properties and spintronics, i.e., heat currents and spin currents.²⁶³ This combination potentially offers benefits in efficiency over traditional Seebeck effect based devices, such as thermoelectric power generators^264,265 for energy harvesting applications.²⁶⁶ A particularly interesting system that is highly studied in the field spin caloritronics is a thin film of heavy metal exhibiting spin-orbit interaction on top of a FM insulator.²⁶⁷ Pt/YIG bilayers are popular candidates chosen to investigate phenomena such as spin pumping^268–271 where the FM YIG is used to drive a spin current into the Pt, which is detected via the inverse Spin Hall effect (ISHE), a manifestation of the spin-orbit interaction.^{194,272–276} These systems are also used to observe the spin Seebeck effect (SSE),^267,277,278 where temperature gradients are used to generate a thermally induced spin voltage in the heavy metal layer, related to the magnetization dynamics of the magnet material in the thermal gradient. Again, the ISHE is used to as a spin current detector to measure the magnitude of the conversion. Of particular interest is the interface between the two layers where investigations have shown that magnetic proximity effects could exist, which have driven intense discussion.^279–282 Here, an induced moment in the nonmagnetic heavy metal layer could convolute the interpreted signal with additional effects such as the anomalous Nernst effect. Further to this, recent x-ray magnetic circular dichroism (XMCD) experiments have led to the debate²⁸³ of the size of such an induced moment of Pt in Pt/YIG samples. Polarized neutron reflectivity (PNR) is an extremely sensitive technique which allows the layered magnetic structure of a material to be probed which has also revealed an induced magnetic moment at the Pt/YIG interface²⁸⁴ in these types of bilayer samples.

Previously, there has been little in the way of local scale analysis/observation of the SSE in Pt/YIG type samples. Local laser heating experiments have been used to observe the effect with a resolution of approximately 5 μm in Hall bar type devices.²⁸⁵ Therefore, it is highly expected that MFM and other relevant techniques (i.e., MFM + MOKE or MFM + SThM) will be used to shed light on the complexity of this type of materials and reveal new insights. Here, high spatial resolution and sensitivity to the perpendicular field gradients could potentially elucidate the magnetic properties and domain structures close to the interface.

Skyrmions are chiral magnetic spin textures that are non-trivial and topologically stable.^286,287 Due to these characteristics, they have been shown to demonstrate interesting phenomena such as the skyrmion Hall effect^173,288 and the topological Hall effect^289–292 and therefore present an interesting platform for investigation of emergent electromagnetism associated with skyrmions. Figures 11(a) and 11(b) show example vector fields for Néel and Bloch skyrmions of certain chirality, respectively, and the color scales depict the z-component of the spin and the insets show a cross-sectional dataset for each skyrmion highlighting the internal spin texture. Skyrmions are known to exist in bulk non-centrosymmetric chiral crystals^66,293–295 and also stabilized in highly engineered thin-films comprising of FM/heavy metal interfaces,²⁹⁶ which can host skyrmions above room temperature.²⁹⁷ Due to the inherent or engineered inversion asymmetry found in these lattices or layered interfaces, a DMI is induced,²⁹¹ which contributes to the overall magnetic ordering and tends to cant neighboring spins in favor of pure parallel/antiparallel Heisenberg exchange interaction, thus generating chiral spin structures. Due to their small size, theorized to range from 1 nm to 1 μm depending on the interplay of mechanisms that stabilize them²⁹⁸ and ability to be generated and manipulated by SOT,^{76,297,299–301} it is expected that skyrmions will give rise to a range of new skyrmionic based logic and storage elements for future computer technologies, which scale beyond dimensions predicted by Moore's law.²⁸⁷ Among other imaging techniques,²⁹⁶ MFM has been used to image skyrmions and estimate the DMI value, as it allows a relatively wide field of view and high resolution to determine parameters such as the domain periodicity which can be used as an input parameter to numerically estimate the average DMI value.³⁰² 

Latest examples of qMFM have highlighted the possibility to attain a deeper understanding of the nanoscale magnetic complexity of skyrmions. Recent developments in implementing quantitative approaches have progressed the use of MFM in skyrmionic research from a simple imaging tool to an integral analysis procedure, which is the key to understanding vital aspects of the magnetic characteristics of skyrmions. Yagil et al. have demonstrated that MFM can be used to study the stray field profile of skyrmions in sputtered Ir/Fe/Co/Pt multilayers.⁷⁵ By employing a closed expression from a multipole expansion and a simulated stray field from the MFM probe, it was demonstrated that fitting the experimental data could reveal insights into the topological properties of the skyrmions. This approach allows for the determination of the skyrmion texture and distinguish between Bloch and Néel type skyrmions, demonstrating with reasonably certainty the prevailing nature of Néel-type skyrmions. Rather than using a simulated MFM probe, Yagil et al.⁷⁵ utilized an alternative approach that can be used to gain an insight into the magnetism on nanometer length scales. Baćani et al. have recently demonstrated through qMFM^130,142 that it is possible to quantify the variation in DMI in sputtered Ir/Co/Pt multilayers to nanoscale precision.³⁰² These observations elucidate the need of the significantly higher current densities required to initiate skyrmionic motion in multilayered systems (∼10¹¹ A m⁻²)²⁹⁷ compared to those in bulk materials (∼10⁶ A m⁻²).²⁹⁸ Here, the authors used the TTF method to calibrate the instruments response, which is required when pushing the limits of the measurement toward the resolution limit of the instrument. This takes into account the physical characteristics of the cantilever, magnetic properties of the MFM probe, and also characteristics specific to the instrument such as the angle at which cantilever is mounted into the system. This method allowed observations of significant inhomogeneity in the DMI values of multilayers, revealing that variations up to 75% of the average value of the DMI can exist in spatial regions of ∼50 nm. Thus, qMFM represents a considerable improvement in understanding of inhomogeneity at a nanoscale level of precision. The authors estimated that this corresponds to variations in the Co layer thickness equal to ±1.2 monolayers, underlying the high level of control required to make skyrmion based memory and logic a reality.

It has recently been demonstrated that not only can MFM play a critical role in the determination of the properties of skyrmions but it can also be used to manipulate the magnetism in thin-films that exhibit DMI and generate skyrmions. Zhang et al.³⁰³ showed that it is possible to use the stray magnetic field from an MFM probe to effectively slice the domain structure in a sample that had an initial starting point in the magnetostatic ground state and displayed a stripe-like domain pattern. By repeatedly scanning the surface, it was possible to cut the stripe domains into skyrmions in the absence of an applied magnetic field and at room temperature. The TTF approach was used to calculate the stray field from the different types of probes used in the experiment to understand the magnitude of the z-component of the magnetic at the sample surface where the interaction occurs.

These examples capture the powerful way in which MFM can be extended by incorporating quantitative methods, such as the TTF approach, to extract information about a sample that is otherwise difficult to achieve. It is expected that as quantitative methods become widespread, a proliferation in these types of insightful experiments will shed light into emerging areas of magnetism at the nanoscale.

Topological insulators (TIs) are unique electronic materials that, in addition to a bulk bandgap similar to an ordinary insulator, have protected conducting states on the edge or surface that are possible due to the combination of spin-orbit interactions and time-reversal symmetry. Besides a huge fundamental interest, ferromagnetic TIs hold a great promise for applications in spintronics, metrology, and quantum computing. However, due to the complexity of sample preparation and cryogenic temperature of operation, so far, relatively limited number of MFM studies have been reported for topological insulators. Wang et al.³⁰⁴ have performed a systematic in situ cryogenic MFM study of FM domains in both single-crystal and thin-films samples of magnetic TIs, Cr-doped (Bi_0.1Sb_0.9)₂Te₃. Bubble-like FM domains were observed in both single crystals and thin films. In the latter, smaller domain size (∼500 nm) with narrower DWs (∼150−300 nm) were detected due to vertical confinement effect, suggesting that thin films are more promising for visualization of chiral edge states.³⁰⁴ In a work by Niu et al.,²²¹ cryogenic MFM was used to study intrinsic ferromagnetism and quantum transport transition in individual Fe-doped Bi₂Se₃ topological insulator NWs. The NW showed spontaneous magnetization with a T_c of 40 K. The intrinsic ferromagnetism and gapped topological surface states in individual NWs suggest a pathway for future memory and ME applications. As the research interest in the field will only grow in near future, the application of advanced MFM modes (i.e., in-field low temperature MFM as well as qMFM) is expected to accelerate to provide valuable information about these fascinating materials.

2D materials are another emerging class of modern artificial materials with exceptionally rich fundamental properties. Creating modern, smart materials with precise control over their physical properties is crucial for a wide range of applications and, as a trend, is most pronounced in the area of atomically thin 2D materials and their heterostructures. Such materials often possess unique and unexpected magnetic properties and MFM is a well-suited tool to validate and study them on nanoscale. For example, low-temperature in-field MFM was applied to studies of ferro-/antiferromagnetic transitions in a quasi-2D itinerant ferromagnet, Fe₃GeTe₂.³⁰⁵ In the local state, it was observed that the branching domain structure dynamically evolved into bubble domains as temperature decreased from 210 to 150 K, demonstrating existence of two distinct stable magnetic transitions and suggesting the existence of an instability in this temperature range.

In another recent study, the authors performed an MFM study of a new material system, which comprises of the InSe semiconductor van der Waals crystal and FM Fe-islands.³⁰⁶ In contrast to many traditional semiconductors, the electronic properties of InSe are preserved after the incorporation of Fe. It was demonstrated that the formation of crystalline Fe-clusters in InSe induces a uniaxial internal magnetic field (∼1 T) perpendicular to the InSe layers. Thus, this hybrid system, which consists of Fe-inclusions and a van der Waals crystal, enables the coexistence of magnetic and semiconducting properties within the same structure.

However, in a number of recent works on 2D materials, MFM was used without applying the correct procedures and control tests, which led to rushed and not experimentally justified conclusions. For example, MFM was applied to characterize the mechanically and liquid exfoliated single- and few-layer MoS₂, graphene, and graphene oxide nanosheets.³⁰⁷ By the analysis of the phase and amplitude shifts, the authors demonstrated that the magnetic response of MoS₂ and graphene is dependent on the layer thickness. It was shown that the mechanically and liquid exfoliated single-layer MoS₂ demonstrated the reverse magnetic signal. At the same time, it was shown that graphene and MoS₂ flakes become nonmagnetic when they exceed a certain thickness. In this initial work, the authors performed merely a simple MFM study and the presence of electrostatic interaction was ruled out only on the basis of separate measurements on Fe₃O₄ and Au nanoparticles rather than directly excluded by the means of active Kelvin compensation. No experiments with probe magnetization reversal were performed and no clear explanation of the effect apart from a possible Li doping of MoS₂ was provided. In the follow-up article by Li and Chen,³⁰⁸ a more methodical and careful experimental study was performed. It was found that the MFM response had significant non-magnetic contributions due to capacitive and electrostatic interactions between the nanosheets of 2D materials and conductive cantilever tip, as demonstrated by EFM and SKPM analyses. In addition, the MFM signals of graphene and MoS₂ nanosheets were not responsive to reversed magnetic moment of the probe. Therefore, the observed MFM response was mainly originated from electrostatic artifacts and not compelling enough to imply intrinsic magnetism in graphene and MoS₂ nanosheets.³⁰⁸ 

Similarly, MFM was used for studies of locally induced magnetization in strained ReSe₂ ribbons.³⁰⁹ The authors observed a big negative phase shift on top of a folded ribbon, which they attributed to strong attractive interaction between the ReSe₂ wrinkles and the MFM probe. However, in this case as well, the conclusions were drawn without a convincing control experiment (i.e., reverse of the probe magnetization, use of non-magnetic metal coated probe, etc.). Similarly to what was discussed earlier, the field of 2D materials in magnetism is extremely fast and successfully growing. While magnetic properties of such materials were somewhat late to be explored (primarily due to difficulty in synthesis of ferromagnets in 2D state), very recent development have demonstrated that this can be successfully overcome, opening the way for advanced MFM studies. As in the case of TI, it can be expected that such varieties of MFM modes as in-field and low temperature MFM as well as qMFM are to be applied.

Using HS-AFM, both the structure and dynamic processes of biomolecules can be observed without disturbing their function.³¹⁰ The possibility to combine this technique with MFM would open new opportunities of characterization and manipulation of biological systems. Also, the combination of AFM and inverted optical microscopy techniques, in particular Total Internal Reflection Fluorescence (TIRF) microscopy, allows for simultaneous manipulation and imaging of samples, which can be applied for the measurement of mechanical properties of single proteins and the identification of specific components in complex assemblies.³¹¹ For that reason, the combination of MFM, capable of, e.g., detection of magnetic labels, and these optical techniques opens the possibility of nanomanipulation and simultaneous detection of different properties giving the chance to obtain information inaccessible with other techniques.

We further discuss the potential to use MFM in less traditional areas such as Life Science and environmental studies. In the case of in vivo applications, MNPs [i.e., superparamagnetic iron oxides (SPIOs)] integrated into the material of a mesh can be used, e.g., for the development of a surgical mesh implant that is visible in magnetic resonance imaging. In order to get a high quality mesh, a narrow size distribution and homogenous spatial distribution, as well as a strong magnetization of SPIOs within the filament of the mesh are required. Slabu et al.³¹² used MFM to determine the beneficial properties for the assembly and imaging of the implant. These analyses showed the feasibility of visualization of surgical implants with incorporated SPIOs and the influence of the agglomeration of SPIOs on their magnetization and on a homogenous spatial distribution within the polymer of the mesh. The findings demonstrate that MFM is a very promising tool for the characterization of surgical implants.

In addition to the traditional use of magnetic materials in high-tech, advanced manufacturing, sensor, and biomedical industries, they are also applied in geoscience, including climate change, pollution evolution, iron biomineralization, and diagenetic processes in sediments.³¹³ Recently, the use of magnetic micro- and nanoparticles has been proposed as a crucial factor for water remediation³¹⁴ and oil recovery.³¹⁵ MFM (together with other characterization techniques) has been applied for a survey of different Fe-containing magnetic compounds targeting their use in environmental applications, such as in wastewater treatments and remediation, and revealing their advantages and drawbacks.³¹⁶ Due to its high resolution and sensitivity, capability to study rough surfaces (i.e., topographic and magnetic signals separation), possibility to detect simultaneously different interactions and properties, and to operate under different ambience conditions and magnetic fields, the MFM is a useful technique to perform magnetic analysis of environmentally relevant systems.

In the Review of the current state of the art, we addressed the recent major developments in the field of MFM, including a variety of the operational modes and new trends in instrumentation, such as in-field and variable field MFM, MFM under controllable temperature, electrostatic compensation, energy dissipation, and MeFM. A variety of specialized, custom-designed magnetic probes (one-side and multilayer coated, functionalized with a MNPs, NWs of CNT filled with magnetic materials, etc.) were presented. Special attention was paid to commonly occurring artifacts in the MFM images and the way to deal with them. Modern objects of recent MFM studies were summarized, including objects such as thin films with PMA, multiferroic materials, and magnetic topological structures.

In this Perspective article, we addressed the emerging MFM trends, concerning further development of instrumentation (e.g., in combination with other SPM modes and radiation techniques) and software, routes toward calibrated MFM imaging using either modeling approaches or physical means of and application of MFM to studies of advanced and emerging materials.

While commercial, off-the-shelf MFM systems still remain a valid indispensable tool for a routine inspection of magnetic properties of samples, modern challenges in both research and industry demand development of new advanced MFM modes. To fulfill this need, the current research is targeted in different directions including the development of a new MFM instrumentation and flexible software; novel types of MFM probes (a key point still under development); the development of multifunctional MFM, through combination with other techniques; and targeting complex material properties, which is a general trend to make MFM compatible with simultaneous transport, thermal, or optical characterization.

Another coming trend is the possibility to obtain volumetric MFM datasets (where the third dimension should be understood in a broad sense, e.g., probe–sample separation, magnetic or electrical field, etc.). This trend is well supported by advances in Big Data acquisition and handling (in part related to the popularity of force-volume functions) and availability of tools/software for 3D-data visualization and analysis. Further development of volumetric MFM (and other SPM modes) is very closely aligned with the development of data-handling practices, statistics, and utilization of machine learning and artificial intelligence. Following this trend, interesting properties could be quantified on the nanoscale without specialized/expensive equipment, e.g., 3D calibrated characterization of a stray field emanating from a nano-object, magnetization dynamics with respect to perturbing fields, or probe calibration/characterization.

Calibrated MFM will remain an important topic for development. While typically macroscopic magnetic field measurements are traceable to nuclear magnetic resonance down to the millimeter scale, here we outlined the need of such metrological procedures with respect to nanoscale characterization as well as the development of capabilities that extend reliable and traceable measurements of spatially resolved magnetic fields down to the micrometer and nanometer scale. We also described a standard procedure for MFM calibration, which represents a comprehensive approach combining the experimental measurements of the reference and measurand samples with the analytical procedure involving image deconvolution in Fourier space using appropriate noise filters (e.g., Wiener Invert Filter) to reconstruct the tip transfer function. We believe that with increasing automation of both measurement capabilities and analysis procedures in modern AFM/MFM instrumentation, qMFM based on the most versatile TTF approach will soon become accessible for routine MFM experiments. The most important requirements along this route are the availability of appropriate reference samples and of dedicated analysis software.

We further discussed the application of MFM to studies of advanced and emerging magnetic materials and structures (often extremely demanding in terms of resolution, sensitivity, and physical environment), namely, antiferromagnets, spin-caloritronic materials, skyrmions, topological insulators, 2D materials, and van der Waals crystals as well as application of MFM to multidisciplinary Life Science and environmental studies, which are often beyond a “traditional” physics approach.

All these examples demonstrate why MFM remains a powerful characterization tool. Equipped with novel modes and additional functionalities, customized MFM is exceptionally well-positioned to become an even more indispensable technique, to be widely used in insightful experiments that will shed light in emerging areas of magnetism at the nanoscale.

Dr. R. Schäfer and Professor R. Cowburn are thanked for their useful insights into the perspectives of the MFM + MOKE multifunctional technique; R. Nevill is acknowledged for assistance in the production of Figs. 1 and 4; and S. Gorno and K. Edmonds are thanked for their assistance in reference management and general suggestions. O.K., R.P., C.B., H.C., and V.N. acknowledge the financial support from the European Metrology Programme for Innovation and Research (Grant No. 15SIB06), NanoMag. M.J. and A.A. acknowledge the support from the Spanish Ministerio de Economia y Competitividad (MINECO) under Project Nos. MAT2015-73775-JIN and MAT2016-76824-C3-1-R.