The twin image-free phase reconstruction is still a challenge with single-shot inline holographic systems. Existing solutions mostly are based on the inverse problem approaches or alternating projections. However, there exists a trade-off between phase retrieval and twin image elimination. Recent studies have introduced a hybrid method involving both the approaches to mitigate this trade-off. Following these works, we propose a single-shot sparsity-assisted iterative phase retrieval approach that applies a sparsity constraint in the object domain and formulates phase retrieval as a minimization problem. We demonstrate lensless digital inline holographic microscopy for imaging transparent and weakly scattering biological samples over a large field-of-view of . The proposed method achieves high fidelity phase reconstruction with faster convergence compared to the existing single-shot phase retrieval methods. We further demonstrate the phase quantification of label-free biological samples, such as cervical cells and RBCs, to highlight the potential of our technique in clinical applications.
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28 December 2022
Research Article|
December 22 2022
Quantitative phase imaging of biological cells using lensless inline holographic microscopy through sparsity-assisted iterative phase retrieval algorithm
Special Collection:
Special Collection Recognizing Women in Applied Physics
Ashwini S. Galande
;
Ashwini S. Galande
(Conceptualization, Data curation, Investigation, Methodology, Resources, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing)
1
Medical Optics and Sensors Laboratory, Department of Biomedical Engineering, Indian Institute of Technology, Hyderabad
, Kandi, Telangana, India
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Hanu Phani Ram Gurram;
Hanu Phani Ram Gurram
(Conceptualization, Data curation, Investigation, Methodology, Resources, Software, Validation, Visualization, Writing – original draft, Writing – review & editing)
1
Medical Optics and Sensors Laboratory, Department of Biomedical Engineering, Indian Institute of Technology, Hyderabad
, Kandi, Telangana, India
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Aruna Priya Kamireddy
;
Aruna Priya Kamireddy
(Data curation, Resources, Validation, Writing – review & editing)
2
Department of Genetics and Molecular Medicine, Kamineni Hospitals
, Hyderabad 500068, India
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Vijaya Sree Venkatapuram
;
Vijaya Sree Venkatapuram
(Data curation)
2
Department of Genetics and Molecular Medicine, Kamineni Hospitals
, Hyderabad 500068, India
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Qurratulain Hasan;
Qurratulain Hasan
(Data curation)
2
Department of Genetics and Molecular Medicine, Kamineni Hospitals
, Hyderabad 500068, India
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Renu John
Renu John
a)
(Conceptualization, Data curation, Methodology, Resources, Supervision, Validation, Writing – review & editing)
1
Medical Optics and Sensors Laboratory, Department of Biomedical Engineering, Indian Institute of Technology, Hyderabad
, Kandi, Telangana, India
a)Author to whom correspondence should be addressed: renujohn@bme.iith.ac.in. URL: https://bme.iith.ac.in/Renuweb/index.html
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a)Author to whom correspondence should be addressed: renujohn@bme.iith.ac.in. URL: https://bme.iith.ac.in/Renuweb/index.html
Note: This paper is part of the Special Collection Recognizing Women in Applied Physics.
J. Appl. Phys. 132, 243102 (2022)
Article history
Received:
August 31 2022
Accepted:
December 05 2022
Citation
Ashwini S. Galande, Hanu Phani Ram Gurram, Aruna Priya Kamireddy, Vijaya Sree Venkatapuram, Qurratulain Hasan, Renu John; Quantitative phase imaging of biological cells using lensless inline holographic microscopy through sparsity-assisted iterative phase retrieval algorithm. J. Appl. Phys. 28 December 2022; 132 (24): 243102. https://doi.org/10.1063/5.0123677
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