Non-specific binding (NSB), especially due to cross-reactivity between specific capture probes and interferents, is a platform-independent issue that limits the detection selectivity of most affinity-based bioanalytical assays. The ability to differentiate and quantify NSB has thus been sought after particularly in label-free biomolecular detections. In this paper, the Langmuir formulation has been applied to understand the transient competitive binding between the target molecules and interferents with the capture probes in complex solutions. By tracking the deviation from the ideal interferent-free case, two NSB differentiation approaches have been proposed. The first one requires multiple measurements during the initial transient and has a short turnaround time. The second approach necessitates only one transient and one equilibrium sampling, which offers a better selectivity over the first approach. The applicability of each approach has been scrutinized depending on whether the interferents would contribute a signal comparable to that of the targets. Finally, the achievable detection selectivity has been correlated to the intrinsic sensitivity of the bioanalytical assay.
Skip Nav Destination
,
Article navigation
15 July 2012
Research Article|
July 23 2012
Transient measurement approaches to differentiate non-specific binding in affinity-based bioanalytical assays
Yufei Mao;
Yufei Mao
1Department of Electrical Engineering,
University of California
, Los Angeles, California 90095, USA
Search for other works by this author on:
Chi On Chui
Chi On Chui
1Department of Electrical Engineering,
University of California
, Los Angeles, California 90095, USA
2California NanoSystems Institute,
University of California
, Los Angeles, California 90095, USA
Search for other works by this author on:
Yufei Mao
1
Chi On Chui
1,2
1Department of Electrical Engineering,
University of California
, Los Angeles, California 90095, USA
2California NanoSystems Institute,
University of California
, Los Angeles, California 90095, USA
J. Appl. Phys. 112, 024702 (2012)
Article history
Received:
March 11 2012
Accepted:
June 27 2012
Citation
Yufei Mao, Chi On Chui; Transient measurement approaches to differentiate non-specific binding in affinity-based bioanalytical assays. J. Appl. Phys. 15 July 2012; 112 (2): 024702. https://doi.org/10.1063/1.4739298
Download citation file:
Pay-Per-View Access
$40.00
Sign In
You could not be signed in. Please check your credentials and make sure you have an active account and try again.
147
Views
Citing articles via
A step-by-step guide to perform x-ray photoelectron spectroscopy
Grzegorz Greczynski, Lars Hultman
Piezoelectric thin films and their applications in MEMS: A review
Jinpeng Liu, Hua Tan, et al.
Decoding diffraction and spectroscopy data with machine learning: A tutorial
D. Vizoso, R. Dingreville
Related Content
Development of mathematical models of lateral flow membrane bioanalytical systems and characterization of the regularities of their functioning
AIP Conf. Proc. (April 2020)
A biodetection method using magnetic particles and micro traps
J. Appl. Phys. (March 2012)
Analyte capture in an array of functionalized droplets for a regenerable biosensor
Biomicrofluidics (September 2019)
Validation of HPLC-UV method for the phenytoin determination in spiked-saliva for TDM application
AIP Conf. Proc. (October 2018)
Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay
Biomicrofluidics (November 2014)