The mechanism and properties of bio-photon emission and absorption in bio-tissues were studied using Pang’s theory of bio-energy transport, in which the energy spectra of protein molecules are obtained from the discrete dynamic equation. From the energy spectra, it was determined that the protein molecules could both radiate and absorb bio-photons with wavelengths of <3 μm and 5–7 μm, consistent with the energy level transitions of the excitons. These results were consistent with the experimental data; this consisted of infrared absorption data from collagen, bovine serum albumin, the protein-like molecule acetanilide, plasma, and a person’s finger, and the laser-Raman spectra of acidity I-type collagen in the lungs of a mouse, and metabolically active Escherichia coli. We further elucidated the mechanism responsible for the non-thermal biological effects produced by the infrared light absorbed by the bio-tissues, using the above results. No temperature rise was observed; instead, the absorbed infrared light promoted the vibrations of amides as well the transport of the bio-energy from one place to other in the protein molecules, which changed their conformations. These experimental results, therefore, not only confirmed the validity of the mechanism of bio-photon emission, and the newly developed theory of bio-energy transport mentioned above, but also explained the mechanism and properties of the non-thermal biological effects produced by the absorption of infrared light by the living systems.
REFERENCES
1.
K. H.
Li
, F. A.
Popp
, W.
Nagl
et al, in Coherent Excitation in Biological Systems
, edited by H.
Frohlich
and F.
Krerner
(Springer-Verlag
, Berlin
, 1983
), pp. 117
–134
.2.
F. A.
Popp
, K. H.
Li
, and Q.
Gu
, Recent Advances and Biophoton in Research and its Application
(World Scientific
, Singapore
, 1992
), pp. 145
–212
.3.
Q.
Gu
and F. A.
Popp
, “Biophoton physics: Potential measure of organizational order
,” in Biological Effects of Light
, edited by E. G.
Jung
and M. F.
Holick
(Walter de Gruyter
, Berlin
, 1994
), pp. 425
–444
.4.
F. A.
Popp
, Electromagnetic Bio-information
, edited by F. A.
Popp
, V.
Warnke
, H. L.
Koning
, W.
Peschka
(Urban & Schuarzenberg Munchen Wien
, Baltimore
, 1979
), pp. 144
–176
.5.
R.
Van Wijk
, H.
Van Aken
, W. P.
Wei
, and F. A.
Popp
, “Light-induced photon emission by mammalian cells
,” J. Photochem. Photobiol. B
18
, 75
(1993
).6.
Q.
Gu
and F. A.
Popp
, “Nonlinear response of biophoton emission to external perturbations
,” Cell. Mol. Life Sci.
48
, 1069
–1082
(1992
).7.
M.-W.
Ho
, F. A.
Popp
, and U.
Warnke
, Bioelectrodynamics and Biocommunication
(World Scientific
, Singapore
, 1994
), pp. 157
–245
.8.
B. J.
Trzebiotowska
, B.
Kochel
, J.
Slawinke
, and W.
Strek
, Photon Emission From Biological Systems
(World Scientific
Singapore
, 1987
).9.
X.-F.
Pang
, “A molecular dynamic theory of ultraweak bio-photon emission in the living systems and its properties
,” Chin. J. At. Mol. Phys.
12
, 411
–420
(1995
);10.
X.-F.
Pang
, “Investigation on molecular mechanism cured sickness for infrared medical instrument
,” Chin. J. Biomed. Eng.
8
, 39
–43
(1999
).11.
X.-F.
Pang
, “A statistical theory for the bio-photon emission of the living systems
,” Chin. J. At. Mol. Phys.
16
, 288
–293
(1997
).12.
X.-F.
Pang
, The Theory of Nonlinear Quantum Mechanics
(Chinese Chongqing
, Chongqing
, 1994
), pp. 567
–634
.13.
X. S.
Chi
, D. B.
Pang
, Y. M.
Li
, H. Y.
Yang
, D. Q.
Ye
, and G. X.
Zhu
, “The measurement of ultraweak luminescence emission from human body surface
,” Chin. J. Biophys.
10
, 165
–168
(1994
).14.
A. S.
Davydov
, “The theory of contraction of proteins under their excitation
,” J. Theor. Biol.
38
, 559
–569
(1973
).15.
A. S.
Davydov
, Solitons in Molecuar Systems
, 2nd ed. (Reidel
, Dordrecht
, 1985
), 1991.16.
P. L.
Christiansen
and A. C.
Scott
, Davydov’s Soliton Revisited
(Plenum
, New York
, 1990
).17.
A. C.
Scott
, “Dynamics of Davydov solitons
,” Phys. Rev. A
26
, 578
–595
(1982
).18.
D. W.
Brown
, “Balancing the Schrodinger equation with Davydovansatze
,” Phys. Rev. A
37
, 5010
–5011
(1988
).19.
D. W.
Brown
, K.
Lindenberg
, and B. J.
West
, “Nonlinear density-matrix equation for the study of finite temperature soliton dynamics
,” Phys. Rev. B
35
, 6169
–6181
(1987
).20.
X.-F.
Pang
, “Soliton motions in organic protein molecules
,” Chin. J. Biochem. Biophys.
18
, 1
–6
(1986
).21.
L.
Cruzeiro
, J.
Halding
, P. L.
Christiansen
, O.
Skovgard
, and A. C.
Scott
, “Temperature effects on the Davydov soliton
,” Phys. Rev. A
37
, 880
–887
(1988
).22.
L.
Cruzeio-Hansson
, “Mechanism of thermal destabilization of the Davydov soliton
,” Phys. Rev. A
45
, 4111
–4115
(1992
).23.
W.
Förner
, “Quantum and disorder effects on Davydov soliton theory
,” Phys. Rev. A
44
, 2694
(1991
).24.
P. S.
Lomdahl
and W. C.
Kerr
, “Do Davydov solitons exist at 300K?
,” Phys. Rev. Lett.
55
, 1235
–1238
(1985
).25.
X.
Wang
, D. W.
Brown
, and K.
Lindenberg
, “Quantum Monte Carlo simulation of the Davydov model
,” Phys. Rev. Lett.
62
, 1796
–1799
(1989
).26.
J. P.
Cottingham
and J. W.
Schweitzer
, “Calculation of the lifetime of a Davydov soliton at finite temperature
,” Phys. Rev. Lett.
62
, 1792
–1795
(1989
).27.
X.-F.
Pang
, “The properties of collective excitation in organic protein molecular system
,” J. Phys. Condens. Matter
2
, 9541
–9556
(1990
).28.
X.-F.
Pang
, “Comment on ‘The thermodynamic properties of -helix protein: A soliton approach,’”
Phys. Rev. E
49
, 4747
–4752
(1994
).29.
X.-F.
Pang
, “Influence of the soliton in anharmonic molecular crystals with temperature on Mossbauer effect
,” Eur. Phys. J. B
10
, 415
–425
(1999
).30.
X.-F.
Pang
, “The effect of Raman scattering accompanied by the soliton excitation occurred in the molecular crystals
,” Physica D
154
, 138
(2001
).31.
X.-F.
Pang
, “Stability of the soliton excited in protein in the biological temperature range
,” Chin. Phys. Lett.
10
, 573
(1993
).32.
X.-F.
Pang
, “Nonlinear vibrational energy-spectra of molecular crystals
,” Chin. Phys.
9
, 106
–111
(2000
).33.
X.-F.
Pang
, “Distribution of vibrational energy-levels of protein molecular chains
,” Commun. Theor. Phys.
35
, 763
(2001
).34.
X.-F.
Pang
, “Mossbauer effect arising from supersonic soliton motion in organic crystal
,” Acta Phys. Sin.
42
, 1841
–1852
(1993
).35.
X.-F.
Pang
, “Thermal stability of the new soliton transported bio-energy under influence of fluctuations of characteristic parameters at biological temperature in the protein molecules
,” Int. J. Mod. Phys. B
19
, 4677
–4699
(2005
).36.
X.-F.
Pang
, H.-W.
Zhang
, J.-F.
Yu
, and Y.-H.
Luo
, “Influences of variations of characteristic parameters arising from the structure nonuniformity of the protein molecules on states of the soliton transported bio-energy in the improved model
,” Int. J. Mod. Phys. B
20
, 3027
(2006
).37.
X.-F.
Pang
and Y.-P.
Feng
, Quantum Mechanics in Nonlinear Systems
(World Scientific
, Singapore
, 2005
).38.
X.-F.
Pang
, “The theory of bio-energy transport in the protein molecules and its properties
,” Phys. Life Rev.
8
, 264
–286
(2011
).39.
X.-F.
Pang
, “Correctness and completeness of the theory of bio-energy transport
,” Phys. Life Rev.
8
, 302
–306
(2011
).40.
X.-F.
Pang
, “An improvement of the Davydov theory of bio-energy etransport in the protein molecular systems
,” Phys. Rev. E
62
, 6989
–6998
(2000
).41.
X.-F.
Pang
, “The lifetime of the soliton in the improved Davydov model at the biological temperature 300K for protein molecules
,” Eur. Phys. J. B
19
, 297
–308
(2001
).42.
X.-F.
Pang
, H.-W.
Zhang
, and Y.-H.
Luo
, “Influences of heat bath and structure disorder in protein molecules on the soliton transported bio-energy in the improved model
,” J. Phys. Condens. Matter
18
, 613
–627
(2006
).43.
X.-F.
Pang
, H.-W.
Zhang
, J.-F.
Yu
, and Y.-P.
Feng
, “States and properties of the soliton transported bio-energy in nonuniform protein molecules at physiological temperature
,” Phys. Lett. A
335
, 408
–415
(2005
).44.
X.-F.
Pang
and Y.-H.
Luo
, “Stabilization of the soliton transported bio-energy in protein molecules in the Improved Model
,” Commun. Theor. Phys.
41
, 470
–476
(2004
).45.
X.-F.
Pang
, J.-F.
Yu
, and Y.-H.
Luo
, “Influences of quantum and disorder effects on solitons exited in protein molecules in improved model
,” Commun. Theor. Phys.
43
, 367
–376
(2005
).46.
X.-F.
Pang
, “The temperature effect of infrared absorption of the protein molecules
,” Int. J. Infrared Millim. Waves
22
, 277
–289
(2001
).47.
X.-F.
Pang
and M.-J.
Liu
, “Features of motion of soliton transported bio-energy in aperiodic α–helix protein molecules with three channels
,” Commun. Theor. Phys.
51
, 170
–180
(2009
).48.
X.-F.
Pang
, “The soliton theory of bio-energy transport in protein molecules
,” in Handbook of Solitons, Research, Technology and Applications
, edited by S. P.
Lang
and S. H.
Bedore
(Nova Science
, New York
, 2009
), pp. 251
–298
.49.
X.-F.
Pang
and M.-J.
Liu
, “Features of motion of soliton transported bio-energy in aperiodic alpha-helix protein molecules with three channels
,” Commun. Theor. Phys.
51
, 170
–180
(2009
).50.
X.-F.
Pang
, “The effects of damping and temperature of medium on the soliton excited in -helix protein molecules with three channels
,” Mod. Phys. Lett. B
23
, 71
–88
(2009
).51.
X.-F.
Pang
and M.-J.
Lui
, “The influences of temperature and chain-chain interaction on features of soliton excited in alpha-helix protein molecules with three channels
,” Int. J. Mod. Phys. B
23
, 2303
–2322
(2009
).52.
X.-F.
Pang
, J.-F.
Yu
, and M.-J.
Liu
, “Changes of properties of the soliton with temperature under influences of structure disorder in the α-helix protein molecules with three channels
,” Mol. Phys.
108
, 1297
–1315
(2010
).53.
X.-F.
Pang
, J.-F.
Yu
, and Y.-H.
Lao
, “Combination effects of structure nonuniformity of proteins on the soliton transported bio-energy
,” Int. J. Mod. Phys. B
21
, 13
–42
(2007
).54.
X.-F.
Pang
and M.-J.
Liu
, “Properties of soliton-transported bio-energy in alpha-helix protein molecules with three channels
,” Commun. Theor. Phys.
48
, 369
–376
(2007
).55.
X.-F.
Pang
, “Theory of bio-energy transport in protein molecules and its experimental evidences as well as applications (A)
,” Fron. Phys. China
2
, 469
–493
(2007
).56.
X.-F.
Pang
, “Influence of structure disorders and temperatures of systems on the bio-energy transport in protein molecules
,” Fron. Phys. China
3
, 457
–488
(2008
).57.
J. C.
Eilbeck
, P. S.
Lomdahl
, and A. C.
Scott
, “The discrete self-trapping equation
,” Physica D
16
, 318
(1985
).58.
J. C.
Eilbeck
, P. S.
Lomdahl
, and A. C.
Scott
, “Soliton structure in crystalline acetanilide
,” Phys. Rev. B
30
, 4073
(1984
).59.
A. C.
Scott
, “Davydov’s soliton
,” Phys. Rep.
217
, 1
(1982
).60.
A. C.
Scott
, E.
Gratton
, E.
Shyamsunder
, and G.
Caren
, “IR overtone spectrum of the vibrational soliton in crystalline acetanilide
,” Phys. Rev. B
32
, 3551
–3553
(1982
).61.
A. C.
Scott
and J. C.
Eilbeck
, “On the CH stretch overtones of benzene
,” Chem. Phys. Lett.
132
, 23
–28
(1986
).62.
A. C.
Scott
, D. S.
Lomdahl
, and J. C.
Eilbeck
, “Between the local-mode and normal-mode limits
,” Chem. Phys. Lett.
113
, 29
(1985
).63.
G.
Careri
, U.
Buontempo
, F.
Galluzzi
, A. C.
Scott
, E.
Gratton
, and E.
Shydmsunder
, “Spectroscopic evidence for Davydov-like solitons in acetanilide
,” Phys. Rev. B
30
, 4689
(1984
).64.
R. E.
Burgeson
, “New collagens, new concepts
,” Annu. Rev. Cell Biol.
4
, 551
(1988
).65.
66.
X.-F.
Pang
, Biophysics
(Press of University of Electronic Science and Technology of China
, Chengdu
, 2007
).67.
X.-F.
Pang
, H.-L.
Xiao
, G.-P.
Cue
, H.-W.
Zhang
, and D.
Bo
, “Experiment studies of properties of infrared absorption of biological tissues
,” Int. J. Infrared Millim. Waves
31
, 521
–532
(2010
).68.
H.-L.
Xiao
, S.-Q.
Sun
, Z.
Qun
, X.-F.
Pang
, and Q.-P.
Chu
, “Study of influence of temperature on the structure of collagen in two-dimensional infrared spectrum
,” Chin. J. At. Mol. Phys.
20
, 81
–85
(2003
).69.
X.-F.
Pang
, “Investigation of nonthermally biological effect of infrared light absorbed by life bodies
,” Phys. Sin.
30
, 525
–532
(2001
).70.
Y. A.
Vladiminov
, Photochemistry and Luminescence of Protein
(Science
, Moskova
, 1965
).71.
72.
73.
X.-F.
Pang
, “Experimental evidences of existence of soliton excited in protein molecules
,” Physics
26
, 665
–667
(1997
).74.
Y. J.
Chen
, L. M.
Li
, and C. L.
Ma
, “Functions of biomacromolecules in the fluorescences of blood plasma
,” Chin. J. Biophys.
9
, 673
–176
(1993
).75.
Q.-P.
Chu
, L.-M.
Chen
, and Q.-N.
Yang
, “The properties of spectrum of collagen and fiber feature of silicosis
,” Chin. J. Sickness Labour-Health
10
, 129
–132
(1992
).76.
B. G.
Frushour
and J. L.
Koenig
, “Raman scattering of collagen, gelatin, and elastin
,” Biopolymers
14
, 379
(1975
).77.
S. J.
Webb
, “Laser-Raman spectroscopy of living cells
,” Phys. Rep.
60
, 201
–224
(1980
).78.
S. J.
Webb
and D. D.
Dobbs
, “Inhibition of bacterial cell growth by 136gc microwaves
,” Nature
218
, 374
–375
(1968
).79.
X.-F.
Pang
, “The biological effect and medical functions of infrared light
,” J. Med. Instrum. World
9
, 36
–39
(2000
).80.
X.-F.
Pang
, “Thermally biological effects and its medical functions of the infrared rays absorbed by living systems
,” Int. J. Infrared Millim. Waves
23
(3
), 375
–391
(2002
).81.
X.-F.
Pang
, “Vibrational energy-spectra of the protein molecules and non-thermally biological effect of infrared lights
,” Int. J. Infrared Millim. Waves
22
, 291
–307
(2001
).82.
X.-F.
Pang
, “Infrared absorption spectra of pure and magnetized water at elevated temperature
,” Eur. Phys. Lett.
92
, 65001
(2010
).83.
X.-F.
Pang
, “The conductivity properties of protons in ice and mechanism of magnetization of liquid water
,” Eur. Phys. J. B
49
, 3
–23
(2006
).84.
C.
Kun
, C.
Xi
, and Z.
Lin
, The Application of Bio-Frequency Spectrum to Medicine
(The peoples Medical Publishing House
, Beijing
, 1995
), pp. 12
–89
.© 2012 American Institute of Physics.
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