In this paper, the interaction between bovine serum albumin (BSA) and zinc oxide (ZnO) nanoparticles was investigated by fluorescence quenching spectra, circular dichroism (CD), and synchronous spectra under physiological conditions. From the analysis of the steady state and time resolved fluorescence quenching of BSA in aqueous solution in presence of ZnO it was observed that the nature of the quenching is of static-type quenching. The Stern–Volmer quenching constant at different temperatures were determined and the thermodynamic parameters , , and were computed. The experiment revealed that the electrostatic interaction was the predominant force in stabilizing the complex. The effect of ZnO on the conformation of BSA has been analyzed by synchronous spectra and CD spectrum. Although the observed results demonstrate some conformational changes in BSA in presence of ZnO nanoparticles, the secondary structure of BSA, predominantly of -helix, is found to retain its identity.
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1 August 2009
Research Article|
August 07 2009
Steady state, time resolved, and circular dichroism spectroscopic studies to reveal the nature of interactions of zinc oxide nanoparticles with transport protein bovine serum albumin and to monitor the possible protein conformational changes
Munmun Bardhan;
Munmun Bardhan
Department of Spectroscopy,
Indian Association for the Cultivation of Science
, Jadavpur, Kolkata 700032, West Bengal, India
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Gopa Mandal;
Gopa Mandal
Department of Spectroscopy,
Indian Association for the Cultivation of Science
, Jadavpur, Kolkata 700032, West Bengal, India
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Tapan Ganguly
Tapan Ganguly
a)
Department of Spectroscopy,
Indian Association for the Cultivation of Science
, Jadavpur, Kolkata 700032, West Bengal, India
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a)
Author to whom correspondence should be addressed. Tel.: +91 33 2473 4971 ext 253. FAX: +91 33 2473 2805. Electronic addresses: [email protected] and [email protected].
J. Appl. Phys. 106, 034701 (2009)
Article history
Received:
January 21 2009
Accepted:
June 29 2009
Citation
Munmun Bardhan, Gopa Mandal, Tapan Ganguly; Steady state, time resolved, and circular dichroism spectroscopic studies to reveal the nature of interactions of zinc oxide nanoparticles with transport protein bovine serum albumin and to monitor the possible protein conformational changes. J. Appl. Phys. 1 August 2009; 106 (3): 034701. https://doi.org/10.1063/1.3190483
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