In theory, groups of enzyme-loaded particles producing an acid or base may show complex behavior including dynamical quorum sensing, the appearance of synchronized oscillations above a critical number or density of particles. Here, experiments were performed with the enzyme urease loaded into mm-sized agarose beads and placed in a solution of urea, resulting in an increase in pH. This behavior was found to be dependent upon the number of beads present in the array; however, reaction-induced convection occurred and plumes of high pH developed that extended to the walls of the reactor. The convection resulted in the motion of the mm-sized particles and conversion of the solution to high pH. Simulations in a simple model of the beads demonstrated the suppression of dynamical quorum sensing in the presence of flow.
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March 2019
Research Article|
March 26 2019
Influence of reaction-induced convection on quorum sensing in enzyme-loaded agarose beads
Vladimir M. Markovic
;
Vladimir M. Markovic
1
Faculty of Physical Chemistry, University of Belgrade
, Studentski trg 12-16, 11158 Belgrade, Serbia
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Tamás Bánsági, Jr.;
Tamás Bánsági, Jr.
2
Department of Chemical and Biological Engineering, The University of Sheffield
, Mappin Street, Sheffield S1 3JD, United Kingdom
3
School of Chemistry, The University of Birmingham
, Edgbaston B15 2TT, United Kingdom
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Dennel McKenzie;
Dennel McKenzie
4
Department of Chemistry, Louisiana State University
, 232 Choppin Hall, Baton Rouge, Louisiana 70803, USA
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Anthony Mai;
Anthony Mai
4
Department of Chemistry, Louisiana State University
, 232 Choppin Hall, Baton Rouge, Louisiana 70803, USA
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John A. Pojman
;
John A. Pojman
a)
4
Department of Chemistry, Louisiana State University
, 232 Choppin Hall, Baton Rouge, Louisiana 70803, USA
a)Authors to whom correspondence should be addressed: john@pojman.com and A.F.Taylor@sheffield.ac.uk
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Annette F. Taylor
Annette F. Taylor
a)
2
Department of Chemical and Biological Engineering, The University of Sheffield
, Mappin Street, Sheffield S1 3JD, United Kingdom
a)Authors to whom correspondence should be addressed: john@pojman.com and A.F.Taylor@sheffield.ac.uk
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a)Authors to whom correspondence should be addressed: john@pojman.com and A.F.Taylor@sheffield.ac.uk
Note: This paper is part of the Focus Issue, “Nonlinear Chemical Dynamics and Its Interdisciplinary Impact: Dedicated to Ken Showalter on the Occasion of his 70th Birthday.”
Chaos 29, 033130 (2019)
Article history
Received:
January 18 2019
Accepted:
March 06 2019
Citation
Vladimir M. Markovic, Tamás Bánsági, Dennel McKenzie, Anthony Mai, John A. Pojman, Annette F. Taylor; Influence of reaction-induced convection on quorum sensing in enzyme-loaded agarose beads. Chaos 1 March 2019; 29 (3): 033130. https://doi.org/10.1063/1.5089295
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