Nanomedicine has a great potential to revolutionize the therapeutic landscape. However, up-to-date results obtained from in vitro experiments predict the in vivo performance of nanoparticles weakly or not at all. There is a need for in vitro experiments that better resemble the in vivo reality. As a result, animal experiments can be reduced, and potent in vivo candidates will not be missed. It is important to gain a deeper knowledge about nanoparticle characteristics in physiological environment. In this context, the protein corona plays a crucial role. Its formation process including driving forces, kinetics, and influencing factors has to be explored in more detail. There exist different methods for the investigation of the protein corona and its impact on physico-chemical and biological properties of nanoparticles, which are compiled and critically reflected in this review article. The obtained information about the protein corona can be exploited to optimize nanoparticles for in vivo application. Still the translation from in vitro to in vivo remains challenging. Functional in vitro screening under physiological conditions such as in full serum, in 3D multicellular spheroids/organoids, or under flow conditions is recommended. Innovative in vivo screening using barcoded nanoparticles can simultaneously test more than hundred samples regarding biodistribution and functional delivery within a single mouse.
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Performance of nanoparticles for biomedical applications: The in vitro/in vivo discrepancy
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March 2022
Review Article|
February 01 2022
Performance of nanoparticles for biomedical applications: The in vitro/in vivo discrepancy
Simone Berger
;
Simone Berger
a)
1
Pharmaceutical Biotechnology, Department of Pharmacy, Ludwig–Maximilians-Universität (LMU) Munich
, Butenandtstr. 5-13, D-81377 Munich, Germany
a)Author to whom correspondence should be addressed: simone.berger@cup.uni-muenchen.de
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Martin Berger
;
Martin Berger
2
Department of Chemistry, Johannes Gutenberg-Universität Mainz
, Duesbergweg 10-14, D-55128 Mainz, Germany
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Christoph Bantz;
Christoph Bantz
3
Fraunhofer Institute for Microengineering and Microsystems IMM
, Carl-Zeiss-Str. 18-20, D-55129 Mainz, Germany
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Michael Maskos;
Michael Maskos
2
Department of Chemistry, Johannes Gutenberg-Universität Mainz
, Duesbergweg 10-14, D-55128 Mainz, Germany
3
Fraunhofer Institute for Microengineering and Microsystems IMM
, Carl-Zeiss-Str. 18-20, D-55129 Mainz, Germany
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Ernst Wagner
Ernst Wagner
1
Pharmaceutical Biotechnology, Department of Pharmacy, Ludwig–Maximilians-Universität (LMU) Munich
, Butenandtstr. 5-13, D-81377 Munich, Germany
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a)Author to whom correspondence should be addressed: simone.berger@cup.uni-muenchen.de
Biophysics Rev. 3, 011303 (2022)
Article history
Received:
September 30 2021
Accepted:
January 04 2022
Citation
Simone Berger, Martin Berger, Christoph Bantz, Michael Maskos, Ernst Wagner; Performance of nanoparticles for biomedical applications: The in vitro/in vivo discrepancy. Biophysics Rev. 1 March 2022; 3 (1): 011303. https://doi.org/10.1063/5.0073494
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