This paper presents a continuous flow microfluidic device for the separation of DNA from blood using magnetophoresis for biological applications and analysis. This microfluidic bio-separation device has several benefits, including decreased sample handling, smaller sample and reagent volumes, faster isolation time, and decreased cost to perform DNA isolation. One of the key features of this device is the use of short-range magnetic field gradients, generated by a micro-patterned nickel array on the bottom surface of the separation channel. In addition, the device utilizes an array of oppositely oriented, external permanent magnets to produce strong long-range field gradients at the interfaces between magnets, further increasing the effectiveness of the device. A comprehensive simulation is performed using COMSOL Multiphysics to study the effect of various parameters on the magnetic flux within the separation channel. Additionally, a microfluidic device is designed, fabricated, and tested to isolate DNA from blood. The results show that the device has the capability of separating DNA from a blood sample with a purity of 1.8 or higher, a yield of up to 33 μg of polymerase chain reaction ready DNA per milliliter of blood, and a volumetric throughput of up to 50 ml/h.
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July 2014
Research Article|
August 22 2014
Magnetophoretic-based microfluidic device for DNA isolation
C. Hale;
C. Hale
Department of Mechanical Engineering,
Southern Illinois University Edwardsville
, Edwardsville, Illinois 62026, USA
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Author to whom correspondence should be addressed. Electronic mail: jdarabi@siue.edu. Tel.: 618-650-3382.
Biomicrofluidics 8, 044118 (2014)
Article history
Received:
June 27 2014
Accepted:
August 12 2014
Citation
C. Hale, J. Darabi; Magnetophoretic-based microfluidic device for DNA isolation. Biomicrofluidics 1 July 2014; 8 (4): 044118. https://doi.org/10.1063/1.4893772
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