This study suggests a new erythrocyte sedimentation rate (ESR) measurement method for the biophysical assessment of blood by using a microfluidic device. For an effective ESR measurement, a disposable syringe filled with blood is turned upside down and aligned at 180° with respect to gravitational direction. When the blood sample is delivered into the microfluidic device from the top position of the syringe, the hematocrit of blood flowing in the microfluidic channel decreases because the red blood cell-depleted region is increased from the top region of the syringe. The variation of hematocrit is evaluated by consecutively capturing images and conducting digital image processing technique for 10 min. The dynamic variation of ESR is quantitatively evaluated using two representative parameters, namely, time constant (λ) and ESR-area (AESR). To check the performance of the proposed method, blood samples with various ESR values are prepared by adding different concentrations of dextran solution. λ and AESR are quantitatively evaluated by using the proposed method and a conventional method, respectively. The proposed method can be used to measure ESR with superior reliability, compared with the conventional method. The proposed method can also be used to quantify ESR of blood collected from malaria-infected mouse under in vivo condition. To indirectly compare with the results obtained by the proposed method, the viscosity and velocity of the blood are measured using the microfluidic device. As a result, the biophysical properties, including ESR and viscosity of blood, are significantly influenced by the parasitemia level. These experimental demonstrations support the notion that the proposed method is capable of effectively monitoring the biophysical properties of blood.
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July 2014
Research Article|
August 05 2014
Microfluidic-based measurement of erythrocyte sedimentation rate for biophysical assessment of blood in an in vivo malaria-infected mouse Available to Purchase
Yang Jun Kang;
Yang Jun Kang
1Department of Mechanical Engineering,
Chosun University
, Gwangju, South Korea
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Young-Ran Ha;
Young-Ran Ha
2Division of Integrative Biosciences and Biotechnology,
Pohang University of Science and Technology
, Pohang, South Korea
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Sang-Joon Lee
Sang-Joon Lee
a)
2Division of Integrative Biosciences and Biotechnology,
Pohang University of Science and Technology
, Pohang, South Korea
3Department of Mechanical Engineering,
Pohang University of Science and Technology
, Pohang, South Korea
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Yang Jun Kang
1
Young-Ran Ha
2
Sang-Joon Lee
2,3,a)
1Department of Mechanical Engineering,
Chosun University
, Gwangju, South Korea
2Division of Integrative Biosciences and Biotechnology,
Pohang University of Science and Technology
, Pohang, South Korea
3Department of Mechanical Engineering,
Pohang University of Science and Technology
, Pohang, South Korea
a)
Present address: San 31, Hyoja-dong, Nam-gu, Pohang, Gyeongbuk 790-784, South Korea. Electronic mail: [email protected]. Fax: +82-54-279-3199.
Biomicrofluidics 8, 044114 (2014)
Article history
Received:
June 17 2014
Accepted:
July 23 2014
Citation
Yang Jun Kang, Young-Ran Ha, Sang-Joon Lee; Microfluidic-based measurement of erythrocyte sedimentation rate for biophysical assessment of blood in an in vivo malaria-infected mouse. Biomicrofluidics 1 July 2014; 8 (4): 044114. https://doi.org/10.1063/1.4892037
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