We report the Laser Induced Forward Transfer (LIFT) of antibodies from a liquid donor film onto paper receivers for application as point-of-care diagnostic sensors. To minimise the loss of functionality of the active biomolecules during transfer, a dynamic release layer was employed to shield the biomaterial from direct exposure to the pulsed laser source. Cellulose paper was chosen as the ideal receiver because of its inherent bio-compatibility, liquid transport properties, wide availability and low cost, all of which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged IgG antibodies were LIFT-printed and their functionality was confirmed via a colorimetric enzyme-linked immunosorbent assay. Localisation of the printed antibodies was exhibited, which can allow the creation of complex 2-d patterns such as QR codes or letters for use in a final working device. Finally, a calibration curve was determined that related the intensity of the colour obtained to the concentration of active antibodies to enable quantitative assessment of the device performance. The motivation for this work was to implement a laser-based procedure for manufacturing low-cost, point-of-care diagnostic devices on paper.
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Research Article|
May 19 2014
Paper-based colorimetric enzyme linked immunosorbent assay fabricated by laser induced forward transfer
Ioannis N. Katis;
Ioannis N. Katis
a)
1Optoelectronics Research Centre,
University of Southampton
, Highfield, Southampton SO17 1BJ, United Kingdom
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Judith A. Holloway;
Judith A. Holloway
2Clinical and Experimental Science, Faculty of Medicine and Institute for Life Sciences,
University of Southampton and NIHR Wellcome Trust Clinical Research Facility and Respiratory Biomedical Research Unit, University Hospital Southampton NHS Foundation Trust
, Southampton, United Kingdom
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Jens Madsen;
Jens Madsen
2Clinical and Experimental Science, Faculty of Medicine and Institute for Life Sciences,
University of Southampton and NIHR Wellcome Trust Clinical Research Facility and Respiratory Biomedical Research Unit, University Hospital Southampton NHS Foundation Trust
, Southampton, United Kingdom
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Saul N. Faust;
Saul N. Faust
2Clinical and Experimental Science, Faculty of Medicine and Institute for Life Sciences,
University of Southampton and NIHR Wellcome Trust Clinical Research Facility and Respiratory Biomedical Research Unit, University Hospital Southampton NHS Foundation Trust
, Southampton, United Kingdom
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Spiros D. Garbis;
Spiros D. Garbis
3Institute for Life Sciences, Centre for Proteomic Research, Cancer Sciences, and Clinical and Experimental Medicine,
University of Southampton
, Highfield Campus, Southampton, United Kingdom
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Peter J. S. Smith;
Peter J. S. Smith
4
Institute for Life Sciences and Centre for Biological Sciences
, University of Southampton, Highfield, Southampton, United Kingdom
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David Voegeli;
David Voegeli
5Faculty of Health Sciences,
University of Southampton
, Highfield, Southampton SO17 1BJ, United Kingdom
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Dan L. Bader;
Dan L. Bader
5Faculty of Health Sciences,
University of Southampton
, Highfield, Southampton SO17 1BJ, United Kingdom
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Robert W. Eason;
Robert W. Eason
1Optoelectronics Research Centre,
University of Southampton
, Highfield, Southampton SO17 1BJ, United Kingdom
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Collin L. Sones
Collin L. Sones
1Optoelectronics Research Centre,
University of Southampton
, Highfield, Southampton SO17 1BJ, United Kingdom
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Ioannis N. Katis
1,a)
Judith A. Holloway
2
Jens Madsen
2
Saul N. Faust
2
Spiros D. Garbis
3
Peter J. S. Smith
4
David Voegeli
5
Dan L. Bader
5
Robert W. Eason
1
Collin L. Sones
1
1Optoelectronics Research Centre,
University of Southampton
, Highfield, Southampton SO17 1BJ, United Kingdom
2Clinical and Experimental Science, Faculty of Medicine and Institute for Life Sciences,
University of Southampton and NIHR Wellcome Trust Clinical Research Facility and Respiratory Biomedical Research Unit, University Hospital Southampton NHS Foundation Trust
, Southampton, United Kingdom
3Institute for Life Sciences, Centre for Proteomic Research, Cancer Sciences, and Clinical and Experimental Medicine,
University of Southampton
, Highfield Campus, Southampton, United Kingdom
4
Institute for Life Sciences and Centre for Biological Sciences
, University of Southampton, Highfield, Southampton, United Kingdom
5Faculty of Health Sciences,
University of Southampton
, Highfield, Southampton SO17 1BJ, United Kingdom
a)
E-mail: [email protected]. Tel.:44 2380 597719.
Biomicrofluidics 8, 036502 (2014)
Article history
Received:
March 05 2014
Accepted:
May 08 2014
Citation
Ioannis N. Katis, Judith A. Holloway, Jens Madsen, Saul N. Faust, Spiros D. Garbis, Peter J. S. Smith, David Voegeli, Dan L. Bader, Robert W. Eason, Collin L. Sones; Paper-based colorimetric enzyme linked immunosorbent assay fabricated by laser induced forward transfer. Biomicrofluidics 1 May 2014; 8 (3): 036502. https://doi.org/10.1063/1.4878696
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