A multi-functional microfluidic platform was fabricated to demonstrate the feasibility of on-chip electroporation integrated with dielectrophoresis (DEP) and alternating-current-electro-osmosis (ACEO) assisted cell/particle manipulation. A spatial gradient of electroporation parameters was generated within a microchamber array and validated using normal human dermal fibroblast (NHDF) cells and red fluorescent protein-expressing human umbilical vein endothelial cells (RFP-HUVECs) with various fluorescent indicators. The edge of the bottom electrode, coinciding with the microchamber entrance, may act as an on-demand gate, functioning under either positive or negative DEP. In addition, at sufficiently low activation frequencies, ACEO vortices can complement the DEP to contribute to a rapid trapping/alignment of particles. As such, results clearly indicate that the microfluidic platform has the potential to achieve high-throughput screening for electroporation with spatial control and uniformity, assisted by DEP and ACEO manipulation/trapping of particles/cells into individual microchambers.
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March 2014
Research Article|
April 24 2014
Individually addressable multi-chamber electroporation platform with dielectrophoresis and alternating-current-electro-osmosis assisted cell positioning
Sinwook Park;
Sinwook Park
Faculty of Mechanical Engineering,
Micro- and Nanofluidics Laboratory, Technion–Israel Institute of Technology
, Technion City 32000, Israel
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Dana Ben Bassat;
Dana Ben Bassat
Faculty of Mechanical Engineering,
Micro- and Nanofluidics Laboratory, Technion–Israel Institute of Technology
, Technion City 32000, Israel
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Gilad Yossifon
Gilad Yossifon
a)
Faculty of Mechanical Engineering,
Micro- and Nanofluidics Laboratory, Technion–Israel Institute of Technology
, Technion City 32000, Israel
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a)
Author to whom correspondence should be addressed. Electronic mail: [email protected]
Biomicrofluidics 8, 024117 (2014)
Article history
Received:
February 28 2014
Accepted:
April 16 2014
Citation
Sinwook Park, Dana Ben Bassat, Gilad Yossifon; Individually addressable multi-chamber electroporation platform with dielectrophoresis and alternating-current-electro-osmosis assisted cell positioning. Biomicrofluidics 1 March 2014; 8 (2): 024117. https://doi.org/10.1063/1.4873439
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