Alternating-current (AC) electrokinetics involve the movement and behaviors of particles or cells. Many applications, including dielectrophoretic manipulations, are dependent upon charge interactions between the cell or particle and the surrounding medium. Medium concentrations are traditionally treated as spatially uniform in both theoretical models and experiments. Human red blood cells (RBCs) are observed to crenate, or shrink due to changing osmotic pressure, over 10 min experiments in non-uniform AC electric fields. Cell crenation magnitude is examined as functions of frequency from 250 kHz to 1 MHz and potential from 10 Vpp to 17.5 Vpp over a 100 μm perpendicular electrode gap. Experimental results show higher peak to peak potential and lower frequency lead to greater cell volume crenation up to a maximum volume loss of 20%. A series of experiments are conducted to elucidate the physical mechanisms behind the red blood cell crenation. Non-uniform and uniform electrode systems as well as high and low ion concentration experiments are compared and illustrate that AC electroporation, system temperature, rapid temperature changes, medium pH, electrode reactions, and convection do not account for the crenation behaviors observed. AC electroosmotic was found to be negligible at these conditions and AC electrothermal fluid flows were found to reduce RBC crenation behaviors. These cell deformations were attributed to medium hypertonicity induced by ion concentration gradients in the spatially nonuniform AC electric fields.
REFERENCES
1.
S. K.
Arya
, K. C.
Lee
, D.
Bin Dah'alan
, Daniel
, and A. R. A.
Rahman
, “Breast tumor cell detection at single cell resolution using an electrochemical impedance technique
,” Lab Chip
12
, 2362
–2368
(2012
).2.
B. H.
Lapizco-Encinas
, B. A.
Simmons
, E. B.
Cummings
, and Y.
Fintschenko
, “Dielectrophoretic concentration and separation of live and dead bacteria in an array of insulators
,” Anal. Chem.
76
, 1571
–1579
(2004
).3.
G.
Medoro
, N.
Manaresi
, A.
Leonardi
, L.
Altomare
, M.
Tartagni
, and R.
Guerrieri
, “A lab-on-a-chip for cell detection and manipulation
,” IEEE Sens. J.
3
, 317
–325
(2003
).4.
S.
Fiedler
, S. G.
Shirley
, T.
Schnelle
, and G.
Fuhr
, “Dielectrophoretic sorting of particles and cells in a microsystem
,” Anal. Chem.
70
, 1909
–1915
(1998
).5.
P. R. C.
Gascoyne
, X. B.
Wang
, Y.
Huang
, and F. F.
Becker
, “Dielectrophoretic separation of cancer cells from blood
,” IEEE Trans. Ind. Appl.
33
, 670
–678
(1997
).6.
A. R.
Minerick
, “The rapidly growing field of micro and nanotechnology to measure living cells
,” AIChE J.
54
, 2230
–2237
(2008
).7.
A. R.
Minerick
, R. H.
Zhou
, P.
Takhistov
, and H. C.
Chang
, “Manipulation and characterization of red blood cells with alternating current fields in microdevices
,” Electrophoresis
24
, 3703
–3717
(2003
).8.
K. M.
Leonard
and A. R.
Minerick
, “Explorations of ABO-Rh antigen expressions on erythrocyte dielectrophoresis: Changes in cross-over frequency
,” Electrophoresis
32
, 2512
–2522
(2011
).9.
S. K.
Srivastava
, A.
Artemiou
, and A. R.
Minerick
, “Direct current insulator-based dielectrophoretic characterization of erythrocytes: ABO-Rh human blood typing
,” Electrophoresis
32
, 2530
–2540
(2011
).10.
G.
Gulati
, Blood Cell Morphology Grading Guide
(American Society for Clinical Pathology (ASCP) Publishing Team
, 2009
).11.
M.
Rasia
and A.
Bollini
, “Red blood cell shape as a function of medium's ionic strength and pH
,” Biochim. Biophys. Acta, Biomembr.
1372
, 198
–204
(1998
).12.
T. M.
Fischer
, “Shape memory of human red blood cells
,” Biophys. J.
86
, 3304
–3313
(2004
).13.
M. M.
Gedde
, E. Y.
Yang
, and W. H.
Huestis
, “Shape response of human erythrocytes to altered cell pH
,” Blood
86
, 1595
–1599
(1995
).14.
K.
Kinosita
and T. Y.
Tsong
, “Formation and resealing of pores of controlled sizes in human erythrocyte-membrane
,” Nature
268
, 438
–441
(1977
).15.
T. Y.
Tsong
and E.
Kingsley
, “Hemolysis of human erythrocyte induced by a rapid temperature jump
,” J. Biol. Chem.
250
, 786
–789
(1975
).16.
S.
Movahed
and D. Q.
Li
, “Microfluidics cell electroporation
,” Microfluid. Nanofluid.
10
, 703
–734
(2011
).17.
P.
Garcia-Sanchez
, A.
Ramos
, A.
Gonzalez
, N. G.
Green
, and H.
Morgan
, “Flow reversal in traveling-wave electrokinetics: An analysis of forces due to ionic concentration gradients
,” Langmuir
25
, 4988
–4997
(2009
).18.
M. L.
Turgeon
, Clinical Hematology: Theory and Procedures
(Lippincott Williams & Wilkins
, 2004
), p. 100
.19.
T. N. G.
Adams
, K. M.
Leonard
, and A. R.
Minerick
, “Frequency sweep rate dependence on the dielectrophoretic response of polystyrene beads and red blood cells
,” Biomicrofluidics
7
, 064114
(2013
).20.
K. A.
DeBruin
and W.
Krassowska
, “Modeling electroporation in a single cell. I. Effects of field strength and rest potential
,” Biophys. J.
77
, 1213
–1224
(1999
).21.
D. C.
Chang
and T. S.
Reese
, “Changes in membrane-structure induced by electroporation as revealed by rapid-freezing electron-microscopy
,” Biophys. J.
58
, 1
–12
(1990
).22.
N.
Bao
, T. T.
Le
, J. X.
Cheng
, and C.
Lu
, “Microfluidic electroporation of tumor and blood cells: Observation of nucleus expansion and implications on selective analysis and purging of circulating tumor cells
,” Integr. Biol.
2
, 113
–120
(2010
).23.
V. V.
Moroz
, A. M.
Chernysh
, E. K.
Kozlova
, P. Y.
Borshegovskaya
, U. A.
Bliznjuk
, R. M.
Rysaeva
, and O. Y.
Gudkova
, “Comparison of red blood cell membrane microstructure after different physicochemical influences: Atomic force microscope research
,” J. Crit. Care
25
, 539
–e1
(2010
).24.
A. O.
Bilska
, K. A.
DeBruin
, and W.
Krassowska
, “Theoretical modeling of the effects of shock duration, frequency, and strength on the degree of electroporation
,” Bioelectrochemistry
51
, 133
–143
(2000
).25.
C. S.
Djuzenova
, U.
Zimmermann
, H.
Frank
, V. L.
Sukhorukov
, E.
Richter
, and G.
Fuhr
, “Effect of medium conductivity and composition on the uptake of propidium iodide into electropermeabilized myeloma cells
,” Biochim. Biophys. Acta, Biomembr.
1284
, 143
–152
(1996
).26.
K. A.
DeBruin
and W.
Krassowska
, “Modeling electroporation in a single cell. II. Effects of ionic concentrations
,” Biophys. J.
77
, 1225
–1233
(1999
).27.
A.
Gencoglu
, F.
Camacho-Alanis
, V. T.
Nguyen
, A.
Nakano
, A.
Ros
, and A. R.
Minerick
, “Quantification of pH gradients and implications in insulator-based dielectrophoresis of biomolecules
,” Electrophoresis
32
, 2436
–2447
(2011
).28.
W. Y.
Ng
, Y. C.
Lam
, and I.
Rodriguez
, “Experimental verification of Faradaic charging in ac electrokinetics
,” Biomicrofluidics
3
, 022405
(2009
).29.
F. E.
Senftle
, J. R.
Grant
, and F. P.
Senftle
, “Low-voltage DC/AC electrolysis of water using porous graphite electrodes
,” Electrochim. Acta
55
, 5148
–5153
(2010
).30.
A. J.
Allmand
and H. C.
Cocks
, “CCCXLVII.-The electrolysis of potassium chloride solutions by alternating currents
,” J. Chem. Soc. (Resumed)
1927
, 2626
–2639
.31.
A.
Ramos
, H.
Morgan
, N. G.
Green
, and A.
Castellanos
, “Ac electrokinetics: A review of forces in microelectrode structures
,” J. Phys. D: Appl. Phys.
31
, 2338
–2353
(1998
).32.
J.
Utoh
and H.
Harasaki
, “Damage to erythrocytes from long-term heat-stress
,” Clin. Sci.
82
, 9
–11
(1992
).33.
S. O.
Sowemimo-Coker
, “Red blood cell hemolysis during processing
,” Transfus. Med. Rev.
16
, 46
–60
(2002
).34.
35.
M.
Lian
, N.
Islam
, and J.
Wu
, “AC electrothermal manipulation of conductive fluids and particles for lab-chip applications
,” IET Nanobiotechnol.
1
, 36
–43
(2007
).36.
Z. R.
Gagnon
and H. C.
Chang
, “Electrothermal ac electro-osmosis
,” Appl. Phys. Lett.
94
, 024101
(2009
).37.
P.
García-Sánchez
, A.
Ramos
, N. G.
Green
, and H.
Morgan
, “Traveling-wave electrokinetic micropumps: Velocity, electrical current, and impedance measurements
,” Langmuir
24
, 9361
–9369
(2008
).38.
D. C.
Grahame
, “Electrode processes and the electrical double layer
,” Annu. Rev. Phys. Chem.
6
, 337
–358
(1955
).39.
H.
Morgan
and N. G.
Green
, AC Electrokinetics: Colloids and Particles
(Research Studies Press Ltd.
, 2003
).40.
W. M.
Deen
, Analysis of Transport Phenomena
, 2 ed. (Oxford University Press
, USA
, 2011
).© 2014 AIP Publishing LLC.
2014
AIP Publishing LLC
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