Deterministic lateral displacement (DLD) is a microfluidic size-based particle separation or filter technology with applications in cell separation and enrichment. Currently, there are no cost-effective manufacturing methods for this promising microfluidic technology. In this fabrication paper, however, we develop a simple, yet robust protocol for thermoplastic DLD devices using regulatory-approved materials and biocompatible methods. The final standalone device allowed for volumetric flow rates of 660 μl min−1 while reducing the manufacturing time to <1 h. Optical profilometry and image analysis were employed to assess manufacturing accuracy and precision; the average replicated post height was 0.48% less than the average post height on the master mold and the average replicated array pitch was 1.1% less than the original design with replicated posts heights of 62.1 ± 5.1 μm (mean ± 6 standard deviations) and replicated array pitches of 35.6 ± 0.31 μm.
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Research Article| September 11 2013
Manufacturing and wetting low-cost microfluidic cell separation devices
Ryan S. Pawell;
David W. Inglis;
Tracie J. Barber;
Ryan S. Pawell, David W. Inglis, Tracie J. Barber, Robert A. Taylor; Manufacturing and wetting low-cost microfluidic cell separation devices. Biomicrofluidics 1 September 2013; 7 (5): 056501. https://doi.org/10.1063/1.4821315
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