A porous silicon (PSi) based microarray has been integrated with a microfluidic system, as a proof of concept device for the optical monitoring of selective label-free DNA-DNA interaction. A 4 × 4 square matrix of PSi one dimensional photonic crystals, each one of 200 μm diameter and spaced by 600 μm, has been sealed by a polydimethylsiloxane (PDMS) channels circuit. The PSi optical microarray elements have been functionalized by DNA single strands after sealing: the microfluidic circuit allows to reduce significantly the biologicals and chemicals consumption, and also the incubation time with respect to a not integrated device. Theoretical calculations, based on finite element method, taking into account molecular interactions, are in good agreement with the experimental results, and the developed numerical model can be used for device optimization. The functionalization process and the interaction between DNA probe and target has been monitored by spectroscopic reflectometry for each PSi element in the microchannels.
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September 2011
Research Article|
August 24 2011
A microfluidics assisted porous silicon array for optical label-free biochemical sensing
Ilaria Rea;
Ilaria Rea
1Institute for Microelectronics and Microsystems - National Council of Research, Naples,
Italy
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Emanuele Orabona;
Emanuele Orabona
1Institute for Microelectronics and Microsystems - National Council of Research, Naples,
Italy
2Department of Physics,
University of Naples “Federico II,” Naples
, Italy
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Annalisa Lamberti;
Annalisa Lamberti
3Department of Biochemistry and Medical Biotechnologies,
University of Naples “Federico II,” Naples
, Italy
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Ivo Rendina;
Ivo Rendina
1Institute for Microelectronics and Microsystems - National Council of Research, Naples,
Italy
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Luca De Stefano
Luca De Stefano
a)
1Institute for Microelectronics and Microsystems - National Council of Research, Naples,
Italy
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a)
Author to whom correspondence should be addressed. Electronic mail: luca.destefano@na.imm.cnr.it. Tel.: + 390816132375. Fax: + 390816132598.
Biomicrofluidics 5, 034120 (2011)
Article history
Received:
April 28 2011
Accepted:
July 28 2011
Citation
Ilaria Rea, Emanuele Orabona, Annalisa Lamberti, Ivo Rendina, Luca De Stefano; A microfluidics assisted porous silicon array for optical label-free biochemical sensing. Biomicrofluidics 1 September 2011; 5 (3): 034120. https://doi.org/10.1063/1.3626008
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