In this study, we propose a microfluidic cell culture device mimicking the microscopic structure in liver tissue called hepatic cords. The cell culture area of the device was designed to align hepatocytes in two lines in a similar way to hepatic cords. Thanks to the structural design together with a cell seeding procedure, rat primary hepatocytes were successfully aligned in two lines and cultured under perfusion condition. It is shown that aligned hepatocytes gradually self-organize and form bile canaliculi along the hepatic cord-like structure. The present technique to culture hepatocytes with functional bile canaliculi could be used as an alternative to animal testing in the field of drug discovery and toxicological studies, and also be beneficial to tissue engineering applications.
Skip Nav Destination
,
,
,
Article navigation
June 2011
Research Article|
June 29 2011
Bile canaliculi formation by aligning rat primary hepatocytes in a microfluidic device
Yosuke Nakao;
Yosuke Nakao
1Institute of Industrial Science,
University of Tokyo
, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
Search for other works by this author on:
Hiroshi Kimura;
Hiroshi Kimura
1Institute of Industrial Science,
University of Tokyo
, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
2Core Research for Evolutional Science and Technology,
Japan Science and Technology Agency
, Tokyo, Japan
Search for other works by this author on:
Yasuyuki Sakai;
Yasuyuki Sakai
1Institute of Industrial Science,
University of Tokyo
, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
2Core Research for Evolutional Science and Technology,
Japan Science and Technology Agency
, Tokyo, Japan
Search for other works by this author on:
Teruo Fujii
Teruo Fujii
a)
1Institute of Industrial Science,
University of Tokyo
, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
2Core Research for Evolutional Science and Technology,
Japan Science and Technology Agency
, Tokyo, Japan
Search for other works by this author on:
Yosuke Nakao
1
Hiroshi Kimura
1,2
Yasuyuki Sakai
1,2
Teruo Fujii
1,2,a)
1Institute of Industrial Science,
University of Tokyo
, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
2Core Research for Evolutional Science and Technology,
Japan Science and Technology Agency
, Tokyo, Japan
a)
Author to whom correspondence should be addressed. Tel.: +81-3-5452-6211. FAX: +81-3-5452-6212. Electronic mail: [email protected].
Biomicrofluidics 5, 022212 (2011)
Article history
Received:
December 13 2010
Accepted:
March 29 2011
Citation
Yosuke Nakao, Hiroshi Kimura, Yasuyuki Sakai, Teruo Fujii; Bile canaliculi formation by aligning rat primary hepatocytes in a microfluidic device. Biomicrofluidics 1 June 2011; 5 (2): 022212. https://doi.org/10.1063/1.3580753
Download citation file:
Pay-Per-View Access
$40.00
Sign In
You could not be signed in. Please check your credentials and make sure you have an active account and try again.
Citing articles via
Improved acoustic holograms using simulated annealing
Gagana Weerasinghe, Bram Servais, et al.
Mechanical interaction between a hydrogel and an embedded cell in biomicrofluidic applications
Lei Li, Jiaqi Zhang, et al.
Design automation for deterministic lateral displacement by leveraging deep Q-network
Yuwei Chen, Yidan Zhang, et al.
Related Content
On-chip three-dimensional cell culture in phaseguides improves hepatocyte functions in vitro
Biomicrofluidics (June 2015)
Microstructured multi-well plate for three-dimensional packed cell seeding and hepatocyte cell culture
Biomicrofluidics (August 2014)
A cell lines derived microfluidic liver model for investigation of hepatotoxicity induced by drug-drug interaction
Biomicrofluidics (March 2019)
Modeling and simulation of interstitial fluid flow around an osteocyte in a lacuno-canalicular network
Physics of Fluids (April 2022)
Electron microscopic and physiological study of diclofenac as oxidative stress on liver and antioxidant marker
AIP Conf. Proc. (December 2023)