This study presents a novel approach to decant supernatant on a centrifugal platform. By manipulating the centrifugally induced pressure and the elastic deformation of the plastic lids in the decanting chamber, fixed amounts of the supernatant can be decanted into the detection chamber at lower rotational speeds. The experimental results showed that decanted volume is affected by the volume of deformation and the operating parameters. Factors that influence the decanting ratio are also discussed. This approach has the advantages of simple design and low manufacturing cost; further, it has no need of surface modification. It has been applied to on-disk separation of plasma from whole blood, and the results showed good stability and repeatability.
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March 2011
Research Article|
March 30 2011
Supernatant decanting on a centrifugal platform
Chih-Hsin Shih;
Chih-Hsin Shih
a)
1Department of Chemical Engineering,
Feng Chia University
, 100 Wenhua Road, Taichung 40724, Taiwan
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Chien-Hsing Lu;
Chien-Hsing Lu
b)
2Department of Obstetrics and Gynecology,
Taichung Veterans General Hospital
, 160 Chung-Kang Rd. Sec. 3, Taichung 40705, Taiwan
; Institute of Biomedical Sciences, National Chung-Hsing University
, Taichung 40227, Taiwan
; and Department of Obstetrics and Gynecology, School of Medicine, National Yang-Ming University
, Taipei 11221, Taiwan
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Wei-Li Yuan;
Wei-Li Yuan
1Department of Chemical Engineering,
Feng Chia University
, 100 Wenhua Road, Taichung 40724, Taiwan
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Wei-Ling Chiang;
Wei-Ling Chiang
1Department of Chemical Engineering,
Feng Chia University
, 100 Wenhua Road, Taichung 40724, Taiwan
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Chia-Hui Lin
Chia-Hui Lin
1Department of Chemical Engineering,
Feng Chia University
, 100 Wenhua Road, Taichung 40724, Taiwan
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a)
Tel.: +886-4-24517250 Ext. 3680. FAX: +886-4-24510890. Electronic mail: chshih@fcu.edu.tw.
b)
Tel.: 886-4-23592525 Ext. 5822. FAX: 886-4-23503021. Electronic mail: chlu@mail.vghtc.gov.tw.
Biomicrofluidics 5, 013414 (2011)
Article history
Received:
January 25 2011
Accepted:
March 09 2011
Citation
Chih-Hsin Shih, Chien-Hsing Lu, Wei-Li Yuan, Wei-Ling Chiang, Chia-Hui Lin; Supernatant decanting on a centrifugal platform. Biomicrofluidics 1 March 2011; 5 (1): 013414. https://doi.org/10.1063/1.3571477
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