This study reports an integrated microfluidic system capable of isolation, counting, and sorting of hematopoietic stem cells (HSCs) from cord blood in an automatic format by utilizing a magnetic-bead-based immunoassay. Three functional modules, including cell isolation, cell counting, and cell sorting modules are integrated on a single chip by using microfluidic technology. The cell isolation module is comprised of a four-membrane-type micromixer for binding of target stem cells and magnetic beads, two pneumatic micropumps for sample transport, and an S-shaped channel for isolation of HSCs using a permanent magnet underneath. The counting and sorting of HSCs are performed by utilizing the cell counting and sorting modules. Experimental results show that a separation efficiency as high as 88% for HSCs from cord blood is achieved within 40 min for a sample volume of 100μl. Therefore, the development of this integrated microfluidic system may be promising for various applications such as stem cell research and cell therapy.

1.
P. S.
Dhot
,
V.
Nair
,
D.
Swarup
,
D.
Sirohi
, and
P.
Ganguli
,
Indian J. Pediatr.
70
,
989
(
2003
).
2.
A.
Tomonari
,
S.
Takahashi
,
K.
Takasugi
,
J.
Ooi
, and
N.
Tsukada
,
Int. J. Hematol.
84
,
438
(
2006
).
3.
K.
Melnik
,
M.
Nakamura
,
K.
Comella
,
L. C.
Lasky
,
M.
Zborowski
, and
J. J.
Chalmers
,
Biotechnol. Prog.
17
,
907
(
2001
).
4.
Y. S.
Chien
,
C. H.
Lin
,
F. J.
Kao
, and
C. W.
Ko
,
Mater. Sci. Forum
505–507
,
643
(
2006
).
5.
K. H.
Han
and
A. B.
Frazier
,
Lab Chip
8
,
1079
(
2008
).
6.
M. S.
Pommer
,
Y.
Zhang
,
N.
Keerthi
,
D.
Chen
,
J. A.
Thomson
,
C. D.
Meinhart
, and
H. T.
Soh
,
Electrophoresis
29
,
1213
(
2008
).
7.
S. Y.
Yang
,
S. K.
Hsiung
,
Y. C.
Hung
,
C. M.
Chang
,
T. L.
Liao
, and
G. B.
Lee
,
Meas. Sci. Technol.
17
,
2001
(
2006
).
8.
S. Y.
Yang
,
K. Y.
Lien
,
K. J.
Huang
,
H. Y.
Lei
, and
G. B.
Lee
,
Biosens. Bioelectron.
24
,
855
(
2008
).
9.
T.
Laurell
,
F.
Petersso
, and
A.
Nilsson
,
Chem. Soc. Rev.
36
,
492
(
2007
).
10.
Z.
Wu
,
A. Q.
Liu
, and
K.
Hjort
,
J. Micromech. Microeng.
17
,
1992
(
2007
).
11.
D.
Huh
,
J. H.
Bahng
,
Y.
Ling
,
H. H.
Wei
,
O. D.
Kripfgans
,
J. B.
Fowlkes
,
J. B.
Grotberg
, and
S.
Takayama
,
Anal. Chem.
79
,
1369
(
2007
).
12.
T.
Baier
,
S.
Mohanty
,
K. S.
Drese
,
F.
Rampf
,
J.
Kim
, and
F.
Schonfeld
,
Microfluid. Nanofluid.
7
,
205
(
2009
).
13.
M. D.
Estes
,
J.
Do
, and
C. H.
Ahn
,
Biomed. Microdevices
11
,
509
(
2009
).
14.
H.
Ueda
,
K. K.
Kajikawa
,
M.
Furuse
,
S.
Fuchino
, and
A.
Ishiyama
,
IEEE Trans. Appl. Supercond.
19
,
2157
(
2009
).
15.
J. W.
Choi
,
K. W.
Oh
,
J. H.
Thomas
,
W. R.
Heineman
,
H. B.
Halsall
,
J. H.
Nevin
,
A. J.
Helmicki
,
H. T.
Henderson
, and
C. H.
Ahn
,
Lab Chip
2
,
27
(
2002
).
16.
Y.
Huang
,
E. L.
Mather
,
L. B.
Janice
, and
M.
Madou
,
Anal. Bioanal. Chem.
372
,
49
(
2002
).
17.
M. A. M.
Gijs
,
Microfluid. Nanofluid.
1
,
22
(
2004
).
18.
K. Y.
Lien
,
J. L.
Lin
,
C. Y.
Liu
,
H. Y.
Lei
, and
G. B.
Lee
,
Lab Chip
7
,
868
(
2007
).
19.
T. M.
McHugh
,
R. C.
Miner
,
L. H.
Logan
,
P.
Daniel
, and
D. P.
Stites
,
J. Clin. Microbiol.
26
,
1957
(
1988
).
20.
S. R.
Elliott
,
P. J.
Macardle
, and
H.
Zola
,
J. Immunol. Methods
217
,
121
(
1998
).
21.
D.
Huh
,
W.
Gu
,
Y.
Kamotani
,
J. B.
Grotberg
, and
S.
Takayama
,
Physiol. Meas
26
,
R73
(
2005
).
22.
C.
Yi
,
Q.
Zhang
,
C. W.
Li
,
J.
Yang
,
J.
Zhao
, and
M.
Yang
,
Anal. Bioanal. Chem.
384
,
1259
(
2006
).
23.
D. A.
Ateya
,
J. S.
Erickson
,
P. B.
Howell
, Jr.
,
L. R.
Hilliard
,
J. P.
Golden
, and
L. F. S.
Ligler
,
Anal. Bioanal. Chem.
391
,
1485
(
2008
).
24.
J. E.
Wharton
,
P.
Jin
,
L. T.
Sexton
,
L. P.
Horne
,
S. A.
Sherrill
,
W. K.
Mino
, and
C. R.
Martin
,
Small
3
,
1424
(
2007
).
25.
A.
Carbonaro
and
L.
Sohn
,
Lab Chip
5
,
1155
(
2005
).
26.
T.
Sun
,
D.
Holmes
,
S.
Gawad
,
N. G.
Green
, and
H.
Morgan
,
Lab Chip
7
,
1034
(
2007
).
27.
Z.
Wang
,
J.
El-Ali
,
M.
Engelund
,
T.
Gotsæd
,
I. R.
Perch-Nielsen
,
K. B.
Mogensen
,
D.
Snakenborg
,
J. P.
Kutter
, and
A.
Wolff
,
Lab Chip
4
,
372
(
2004
).
28.
L. M.
Fu
,
R. J.
Yang
,
C. H.
Lin
,
Y. J.
Pan
, and
G. B.
Lee
,
Anal. Chim. Acta
507
,
163
(
2004
).
29.
C. M.
Chang
,
K.
Hsiung
, and
G. B.
Lee
,
Mater. Sci. Forum
505–507
,
637
(
2005
).
30.
C. H.
Lin
and
G. B.
Lee
,
J. Micromech. Microeng.
13
,
447
(
2003
).
31.
Y. N.
Wang
,
Y.
Kang
,
D.
Xu
,
C. H.
Chon
,
L.
Barnett
,
S. A.
Kalam
, and
D.
Li
,
Lab Chip
8
,
309
(
2008
).
32.
B. I.
Haukanes
and
C.
Kvam
,
Biotechnology
11
,
60
(
1993
).
33.
Y. F.
Lee
,
K. Y.
Lien
,
H. Y.
Lei
, and
G. B.
Lee
,
Biosens. Bioelectron.
25
,
745
(
2009
).
34.
C. H.
Wang
and
G. B.
Lee
,
J. Micromech. Microeng.
16
,
341
(
2006
).
35.
Y. N.
Yang
,
H. I.
Lin
,
J. H.
Wang
,
S. C.
Shiesh
, and
G. B.
Lee
,
Biosens. Bioelectron.
24
,
3091
(
2009
).
36.
P.
Aroviita
,
K.
Teramo
,
V.
Hiilesmaa
, and
R.
Kekomäki
,
Transfusion (Bethesda, Md.)
45
,
613
(
2005
).
You do not currently have access to this content.