This study reports an integrated microfluidic system capable of isolation, counting, and sorting of hematopoietic stem cells (HSCs) from cord blood in an automatic format by utilizing a magnetic-bead-based immunoassay. Three functional modules, including cell isolation, cell counting, and cell sorting modules are integrated on a single chip by using microfluidic technology. The cell isolation module is comprised of a four-membrane-type micromixer for binding of target stem cells and magnetic beads, two pneumatic micropumps for sample transport, and an S-shaped channel for isolation of HSCs using a permanent magnet underneath. The counting and sorting of HSCs are performed by utilizing the cell counting and sorting modules. Experimental results show that a separation efficiency as high as 88% for HSCs from cord blood is achieved within 40 min for a sample volume of . Therefore, the development of this integrated microfluidic system may be promising for various applications such as stem cell research and cell therapy.
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June 2010
Research Article|
June 24 2010
An integrated microfluidic system for isolation, counting, and sorting of hematopoietic stem cells
Huei-Wen Wu;
Huei-Wen Wu
1Department of Engineering Science,
National Cheng Kung University
, Tainan 701, Taiwan
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Ruo-Chi Hsu;
Ruo-Chi Hsu
1Department of Engineering Science,
National Cheng Kung University
, Tainan 701, Taiwan
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Chun-Che Lin;
Chun-Che Lin
1Department of Engineering Science,
National Cheng Kung University
, Tainan 701, Taiwan
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Shiaw-Min Hwang;
Shiaw-Min Hwang
2Bioresource Collection and Research Center,
Food Industry Research and Development Institute
, Hsinchu 300, Taiwan
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Gwo-Bin Lee
Gwo-Bin Lee
a)
1Department of Engineering Science,
National Cheng Kung University
, Tainan 701, Taiwan
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a)
Author to whom correspondence should be addressed. Electronic mail: [email protected]. Tel.: +886-6-2757575 ext 63347. Tel./FAX: +886-6-2761687.
Biomicrofluidics 4, 024112 (2010)
Article history
Received:
February 24 2010
Accepted:
May 26 2010
Citation
Huei-Wen Wu, Ruo-Chi Hsu, Chun-Che Lin, Shiaw-Min Hwang, Gwo-Bin Lee; An integrated microfluidic system for isolation, counting, and sorting of hematopoietic stem cells. Biomicrofluidics 1 June 2010; 4 (2): 024112. https://doi.org/10.1063/1.3454767
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