Surface acoustic waves (SAWs) have been used as a rapid and efficient technique for driving microparticles into a three-dimensional scaffold matrix, raising the possibility that SAW may be effective in seeding live cells into scaffolds, that is, if the cells were able to survive the infusion process. Primary osteoblast-like cells were used to specifically address this issue: To investigate the effects of SAW on the cells’ viability, proliferation, and differentiation. Fluorescence-labeled osteoblast-like cells were seeded into polycaprolactone scaffolds using the SAW method with a static method as a control. The cell distribution in the scaffold was assessed through image analysis. The cells were far more uniformly driven into the scaffold with the SAW method compared to the control, and the seeding process with SAW was also significantly faster: Cells were delivered into the scaffold in seconds compared to the hour-long process of static seeding. Over 80% of the osteoblast-like cells were found to be viable after being treated with SAW at 20 MHz for 10–30 s with an applied power of 380 mW over a wide range of cell suspension volumes and cell densities . After determining the optimal cell seeding parameters, we further found that the treated cells offered the same functionality as untreated cells. Taken together, these results show that the SAW method has significant potential as a practical scaffold cell seeding method for tissue and orthopedic engineering.
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September 2009
Research Article|
August 03 2009
Effect of surface acoustic waves on the viability, proliferation and differentiation of primary osteoblast-like cells
Haiyan Li;
Haiyan Li
1Department of Mechanical and Aerospace Engineering, MicroNanophysics Research Laboratory,
Monash University
, Melbourne, Victoria 3800, Australia
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James Friend;
James Friend
1Department of Mechanical and Aerospace Engineering, MicroNanophysics Research Laboratory,
Monash University
, Melbourne, Victoria 3800, Australia
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Leslie Yeo;
Leslie Yeo
1Department of Mechanical and Aerospace Engineering, MicroNanophysics Research Laboratory,
Monash University
, Melbourne, Victoria 3800, Australia
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Ayan Dasvarma;
Ayan Dasvarma
2
Australian Stem Cell Centre
, Clayton, Victoria 3166, Australia
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Kathy Traianedes
Kathy Traianedes
2
Australian Stem Cell Centre
, Clayton, Victoria 3166, Australia
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Haiyan Li
1
James Friend
1
Leslie Yeo
1
Ayan Dasvarma
2
Kathy Traianedes
2
1Department of Mechanical and Aerospace Engineering, MicroNanophysics Research Laboratory,
Monash University
, Melbourne, Victoria 3800, Australia
2
Australian Stem Cell Centre
, Clayton, Victoria 3166, Australia
Biomicrofluidics 3, 034102 (2009)
Article history
Received:
May 14 2009
Accepted:
July 10 2009
Citation
Haiyan Li, James Friend, Leslie Yeo, Ayan Dasvarma, Kathy Traianedes; Effect of surface acoustic waves on the viability, proliferation and differentiation of primary osteoblast-like cells. Biomicrofluidics 1 September 2009; 3 (3): 034102. https://doi.org/10.1063/1.3194282
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