Single-cell printing technology has arisen as a potent instrument for investigating cell biology and disease pathophysiology. Nonetheless, current single-cell printing methodologies are hindered by restricted throughput, a limited field of view, and diminished efficiency. We present an innovative single-cell printing chip that utilizes thermal inkjet technology for single-cell printing, therefore addressing these constraints. We have accomplished high-throughput, wide-field, and efficient single-cell printing by merging a high-density thermal foam-based inkjet nozzle array on a chip with high-speed cameras and computer vision technologies for optical image capture and single-cell identification training. We have shown the efficacy and adaptability of the printing chip by printing various concentrations of Chinese hamster ovary cells and human embryonic kidney 293 cells. The printing of a single 96-well plate is accomplished in 2–3 min, facilitating one-time loading and uninterrupted multi-plate paving. Our thermal bubble single-cell printing chip serves as a viable platform for high-throughput single-cell analysis applications.
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December 2024
Research Article|
November 26 2024
Thermal bubble single-cell printing chip: High-throughput, wide-field, and efficient
Bo Deng
;
Bo Deng
(Data curation, Investigation, Methodology, Project administration, Validation, Writing – original draft, Writing – review & editing)
1
School of Microelectronics, Shanghai University
, Shanghai 201800, China
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Kun Wang
;
Kun Wang
(Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing)
2
Shanghai Aurefluidics Technology Co. Ltd
, Shanghai 201800, China
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Peng Huang;
Peng Huang
(Investigation, Project administration, Resources, Supervision, Validation)
2
Shanghai Aurefluidics Technology Co. Ltd
, Shanghai 201800, China
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Miaomiao Yang;
Miaomiao Yang
(Resources, Software)
2
Shanghai Aurefluidics Technology Co. Ltd
, Shanghai 201800, China
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Demeng Liu
;
Demeng Liu
(Funding acquisition, Investigation, Project administration, Resources, Validation, Writing – original draft, Writing – review & editing)
1
School of Microelectronics, Shanghai University
, Shanghai 201800, China
2
Shanghai Aurefluidics Technology Co. Ltd
, Shanghai 201800, China
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Yimin Guan
Yimin Guan
a)
(Conceptualization, Funding acquisition, Project administration, Resources)
1
School of Microelectronics, Shanghai University
, Shanghai 201800, China
2
Shanghai Aurefluidics Technology Co. Ltd
, Shanghai 201800, China
a)Author to whom correspondence should be addressed: Yimin_Guan@shu.edu.cn
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a)Author to whom correspondence should be addressed: Yimin_Guan@shu.edu.cn
Biomicrofluidics 18, 064102 (2024)
Article history
Received:
June 27 2024
Accepted:
November 01 2024
Citation
Bo Deng, Kun Wang, Peng Huang, Miaomiao Yang, Demeng Liu, Yimin Guan; Thermal bubble single-cell printing chip: High-throughput, wide-field, and efficient. Biomicrofluidics 1 December 2024; 18 (6): 064102. https://doi.org/10.1063/5.0225883
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