We propose a traveling surface acoustic wave (TSAW)-based microfluidic method for cell lysis that enables lysis of any biological entity, without the need for additional additives. Lysis of cells in the sample solution flowing through a poly (dimethyl siloxane) microchannel is enabled by the interaction of cells with TSAWs propagated from gold interdigitated transducers (IDTs) patterned onto a LiNbO3 piezoelectric substrate, onto which the microchannel was also bonded. Numerical simulations to determine the wave propagation intensities with varying parameters including IDT design, supply voltage, and distance of the channel from the IDT were performed. Experiments were then used to validate the simulations and the best lysis parameters were used to maximize the nucleic acid/protein extraction efficiency (>95%) within few seconds. A comparative analysis of our method with traditional chemical, physical and thermal, as well as the current microfluidic methods for lysis demonstrates the superiority of our method. Our lysis strategy can hence be used independently and/or integrated with other nucleic acid-based technologies or point-of-care devices for the lysis of any pathogen (Gram positives and negatives), eukaryotic cells, and tissues at low voltage (3 V) and frequency (33.17 MHz), without the use of amplifiers.
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