Systematic evolution of ligands by exponential enrichment (SELEX) is a method that is generally used for developing aptamers, which have arisen the promising alternatives for antibodies. However, conventional SELEX methods have limitations, such as a limited selection of target molecules, time-consuming and complex fabrication processes, and labor-intensive processes, which result in low selection yields. Here, we used (i) graphene oxide (GO)-coated magnetic nanoparticles in the selection process for separation and label-free detection and (ii) a multilayered microfluidic device manufactured using a three-dimensionally printed mold that is equipped with automated control valves to achieve precise fluid flows. The developed on-chip aptamer selection device and GO-coated magnetic nanoparticles were used to screen aptamer candidates for adenosine in eight cycles of the selection process within approximately 2 h for each cycle. Based on results from isothermal titration calorimetry, an aptamer with a dissociation constant of 18.6 ± 1.5 μM was selected. Therefore, the on-chip platform based on GO-coated magnetic nanoparticles provides a novel label-free screening technology for biosensors and micro/nanobiotechnology for achieving high-quality aptamers.
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July 2022
Research Article|
July 28 2022
On-chip selection of adenosine aptamer using graphene oxide-coated magnetic nanoparticles
Hosub Lim
;
Hosub Lim
(Conceptualization, Investigation, Writing – original draft, Writing – review and editing)
1
Harvard Institute of Medicine, Harvard Medical School, Harvard University, Brigham and Women's Hospital
, Boston, Massachusetts 02115, USA
2
Institute of Quantum Biophysics (IQB), Sungkyunkwan University
, Suwon, Republic of Korea
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Junhyuck Chang
;
Junhyuck Chang
(Conceptualization, Investigation, Writing – original draft, Writing – review and editing)
3School of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, Republic of Korea
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Kyung-il Kim;
Kyung-il Kim
(Investigation, Writing – original draft)
3School of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, Republic of Korea
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Youngkwang Moon;
Youngkwang Moon
(Investigation)
4
School of Mechanical Engineering, Sungkyunkwan University
, Suwon, Republic of Korea
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Saebom Lee;
Saebom Lee
(Investigation)
4
School of Mechanical Engineering, Sungkyunkwan University
, Suwon, Republic of Korea
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Byoungsang Lee
;
Byoungsang Lee
(Investigation)
3School of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, Republic of Korea
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Jung Heon Lee
;
Jung Heon Lee
a)
(Conceptualization, Funding acquisition, Supervision, Writing – original draft, Writing – review and editing)
2
Institute of Quantum Biophysics (IQB), Sungkyunkwan University
, Suwon, Republic of Korea
3School of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, Republic of Korea
5
Research Center for Advanced Materials Technology, Sungkyunkwan University
, Suwon, Republic of Korea
6
Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University (SKKU)
, Suwon 16419, Republic of Korea
a)Authors to whom correspondence should be addressed: jhlee7@skku.edu, Tel.: +82-31-290-7404 and lee.jinkee@skku.edu, Tel.: +82-31-299-4845.
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Jinkee Lee
Jinkee Lee
a)
(Conceptualization, Funding acquisition, Supervision, Writing – original draft, Writing – review and editing)
2
Institute of Quantum Biophysics (IQB), Sungkyunkwan University
, Suwon, Republic of Korea
4
School of Mechanical Engineering, Sungkyunkwan University
, Suwon, Republic of Korea
a)Authors to whom correspondence should be addressed: jhlee7@skku.edu, Tel.: +82-31-290-7404 and lee.jinkee@skku.edu, Tel.: +82-31-299-4845.
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a)Authors to whom correspondence should be addressed: jhlee7@skku.edu, Tel.: +82-31-290-7404 and lee.jinkee@skku.edu, Tel.: +82-31-299-4845.
Biomicrofluidics 16, 044102 (2022)
Article history
Received:
April 10 2022
Accepted:
June 30 2022
Citation
Hosub Lim, Junhyuck Chang, Kyung-il Kim, Youngkwang Moon, Saebom Lee, Byoungsang Lee, Jung Heon Lee, Jinkee Lee; On-chip selection of adenosine aptamer using graphene oxide-coated magnetic nanoparticles. Biomicrofluidics 1 July 2022; 16 (4): 044102. https://doi.org/10.1063/5.0095419
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