A low-cost device for registration-free quantitative phase microscopy (QPM) based on the transport of intensity equation of cells in continuous flow is presented. The method uses acoustic focusing to align cells into a single plane where all cells move at a constant speed. The acoustic focusing plane is tilted with respect to the microscope’s focal plane in order to obtain cell images at multiple focal positions. As the cells are displaced at constant speed, phase maps can be generated without the need to segment and register individual objects. The proposed inclined geometry allows for the acquisition of a vertical stack without the need for any moving part, and it enables a cost-effective and robust implementation of QPM. The suitability of the solution for biological imaging is tested on blood samples, demonstrating the ability to recover the phase map of single red blood cells flowing through the microchip.
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Acoustofluidic phase microscopy in a tilted segmentation-free configuration
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January 2021
Research Article|
January 05 2021
Acoustofluidic phase microscopy in a tilted segmentation-free configuration
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Julián Mejía Morales
;
Julián Mejía Morales
a)
1
Institut de Physique de Nice, Université Côte d’Azur, CNRS
, 06560 Valbonne, France
2
Department of Experimental Medicine, University of Genova
, 16149 Genova, Italy
a)Author to whom correspondence should be addressed: [email protected]
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Björn Hammarström
;
Björn Hammarström
3
Engineering Sciences, University of Southampton
, SO17 1BJ Southampton, United Kingdom
4
KTH Royal Institute of Technology
, 100 44 Stockholm, Sweden
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Gian Luca Lippi
;
Gian Luca Lippi
1
Institut de Physique de Nice, Université Côte d’Azur, CNRS
, 06560 Valbonne, France
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Massimo Vassalli
;
Massimo Vassalli
5
James Watt School of Engineering, University of Glasgow
, G12 8LT Glasgow, United Kingdom
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Peter Glynne-Jones
Peter Glynne-Jones
3
Engineering Sciences, University of Southampton
, SO17 1BJ Southampton, United Kingdom
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Julián Mejía Morales
1,2,a)
Björn Hammarström
3,4
Gian Luca Lippi
1
Massimo Vassalli
5
Peter Glynne-Jones
3
1
Institut de Physique de Nice, Université Côte d’Azur, CNRS
, 06560 Valbonne, France
2
Department of Experimental Medicine, University of Genova
, 16149 Genova, Italy
3
Engineering Sciences, University of Southampton
, SO17 1BJ Southampton, United Kingdom
4
KTH Royal Institute of Technology
, 100 44 Stockholm, Sweden
5
James Watt School of Engineering, University of Glasgow
, G12 8LT Glasgow, United Kingdom
a)Author to whom correspondence should be addressed: [email protected]
Biomicrofluidics 15, 014102 (2021)
Article history
Received:
November 06 2020
Accepted:
December 17 2020
Citation
Julián Mejía Morales, Björn Hammarström, Gian Luca Lippi, Massimo Vassalli, Peter Glynne-Jones; Acoustofluidic phase microscopy in a tilted segmentation-free configuration. Biomicrofluidics 1 January 2021; 15 (1): 014102. https://doi.org/10.1063/5.0036585
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