Multiple myeloma (MM), the disorder of plasma cells, is the second most common type of hematological cancer and is responsible for approximately 20% of deaths from hematological malignancies. The current gold standard for MM diagnosis includes invasive bone marrow aspiration. However, it lacks the sensitivity to detect minimal residual disease, and the nonuniform distribution of clonal plasma cells (CPCs) within bone marrow also often results in inaccurate reporting. Serum and urine assessment of monoclonal proteins, such as Kappa light chains, is another commonly used approach for MM diagnosis. Although it is noninvasive, the level of paraprotein elevation is still too low for detecting minimal residual disease and nonsecretive MM. Circulating CPCs (cCPCs) have been reported to be present in the peripheral blood of MM patients, and high levels of cCPCs were shown to correlate with poor survival. This suggests a potential noninvasive approach for MM disease progress monitoring and prognosis. In this study, we developed a mechanical property-based microfluidic platform to capture cCPCs. Using human myeloma cancer cell lines spiked in healthy donor blood, the microfluidic platform demonstrates high enrichment ratio (>500) and sufficient capture efficiency (40%–55%). Patient samples were also assessed to investigate the diagnostic potential of cCPCs for MM by correlating with the levels of Kappa light chains in patients.
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Mechanical segregation and capturing of clonal circulating plasma cells in multiple myeloma using micropillar-integrated microfluidic device
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November 2019
Research Article|
November 19 2019
Mechanical segregation and capturing of clonal circulating plasma cells in multiple myeloma using micropillar-integrated microfluidic device
Dongfang Ouyang;
Dongfang Ouyang
a)
1
Department of Mechanical and Industrial Engineering, University of Toronto
, Toronto, Ontario M5S 3G8, Canada
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Yonghua Li;
Yonghua Li
a)
2
Department of Hematology, General Hospital of Southern Theater Command, PLA
, Guangzhou 510010, China
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Wenqi He;
Wenqi He
3
Department of Biomedical Engineering, Southern University of Science and Technology
, Shenzhen 518055, China
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Weicong Lin
;
Weicong Lin
3
Department of Biomedical Engineering, Southern University of Science and Technology
, Shenzhen 518055, China
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Lina Hu;
Lina Hu
4
Department of Hematology, Shenzhen People's Hospital
, Shenzhen 518020, China
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Chen Wang;
Chen Wang
5
Pathology and Laboratory Medicine, Mount Sinai Hospital
, Toronto, Ontario M5G 1X5, Canada
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Liangcheng Xu;
Liangcheng Xu
6
Institute of Biomaterials & Biomedical Engineering, University of Toronto
, Toronto, Ontario M5S 3G9, Canada
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Jaewon Park;
Jaewon Park
b)
3
Department of Biomedical Engineering, Southern University of Science and Technology
, Shenzhen 518055, China
7
Department of Electrical and Electronic Engineering, Southern University of Science and Technology
, Shenzhen 518055, China
b)Authors to whom correspondence should be addressed: youlidan@mie.utoronto.ca, Tel.: +1 416-978-5736 and jwpark@sustech.edu.cn, Tel.: +86 755-8801-8574.
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Lidan You
Lidan You
b)
1
Department of Mechanical and Industrial Engineering, University of Toronto
, Toronto, Ontario M5S 3G8, Canada
6
Institute of Biomaterials & Biomedical Engineering, University of Toronto
, Toronto, Ontario M5S 3G9, Canada
b)Authors to whom correspondence should be addressed: youlidan@mie.utoronto.ca, Tel.: +1 416-978-5736 and jwpark@sustech.edu.cn, Tel.: +86 755-8801-8574.
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a)
Contributions: Dongfang Ouyang and Yonghua Li contributed equally to this work.
b)Authors to whom correspondence should be addressed: youlidan@mie.utoronto.ca, Tel.: +1 416-978-5736 and jwpark@sustech.edu.cn, Tel.: +86 755-8801-8574.
Biomicrofluidics 13, 064114 (2019)
Article history
Received:
June 04 2019
Accepted:
October 08 2019
Citation
Dongfang Ouyang, Yonghua Li, Wenqi He, Weicong Lin, Lina Hu, Chen Wang, Liangcheng Xu, Jaewon Park, Lidan You; Mechanical segregation and capturing of clonal circulating plasma cells in multiple myeloma using micropillar-integrated microfluidic device. Biomicrofluidics 1 November 2019; 13 (6): 064114. https://doi.org/10.1063/1.5112050
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