We present here a novel microfluidic platform that can perform microfluidic on-chip immunohistochemistry (IHC) processes on a formalin-fixed paraffin-embedded section slide. Unlike previous microfluidic IHC studies, our microfluidic chip made of organic solvent-resistant polyurethane acrylate (PUA) is capable of conducting on-chip IHC processes consecutively. A narrow channel wall structure of the PUA chip shows effective sealing by pressure-based reversible assembly with a section slide. We performed both on-chip IHC and conventional IHC processes and compared the IHC results based on the immunostaining intensity. The result showed that the effects of the on-chip deparaffinization, antigen retrieval, and immunoreaction processes on the IHC result were equivalent to conventional methods while reducing the total process time to less than 1/2. The experiment with breast cancer tissue shows that human epidermal growth factor receptor 2 (HER2) classification can be performed by obtaining a clearly distinguishable immunostaining intensity according to the HER2 expression level. We expect our on-chip microfluidic platform to provide a facile technique suitable for miniaturized, automated, and precise diagnostic devices, including a point-of-care device.
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July 2018
Research Article|
July 19 2018
Microfluidic on-chip immunohistochemistry directly from a paraffin-embedded section
Chang Hyun Cho;
Chang Hyun Cho
1
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)
, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
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Seyong Kwon;
Seyong Kwon
1
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)
, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
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Segi Kim;
Segi Kim
1
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)
, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
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Yoonmi Hong;
Yoonmi Hong
1
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)
, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
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Pilnam Kim
;
Pilnam Kim
1
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)
, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
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Eun Sook Lee;
Eun Sook Lee
2
Center for Breast Cancer, National Cancer Center
, 323 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 10408, Republic of Korea
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Je-Kyun Park
Je-Kyun Park
a)
1
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)
, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
a)Author to whom correspondence should be addressed: jekyun@kaist.ac.kr. Tel.: +82-42-350-4315. Fax: +82-42-350-4310.
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a)Author to whom correspondence should be addressed: jekyun@kaist.ac.kr. Tel.: +82-42-350-4315. Fax: +82-42-350-4310.
Biomicrofluidics 12, 044110 (2018)
Article history
Received:
May 31 2018
Accepted:
July 09 2018
Citation
Chang Hyun Cho, Seyong Kwon, Segi Kim, Yoonmi Hong, Pilnam Kim, Eun Sook Lee, Je-Kyun Park; Microfluidic on-chip immunohistochemistry directly from a paraffin-embedded section. Biomicrofluidics 1 July 2018; 12 (4): 044110. https://doi.org/10.1063/1.5042347
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