An ion concentration polarization (ICP)-based electrokinetic concentration device is used for accelerating the surface hybridization reaction between exosomal microRNAs (miRNAs) and morpholinos (MOs) as a synthetic oligo capture probe in the nanomolar concentration range in a microfluidic channel. Compared with standard hybridization at the same concentration, the hybridization time of the miRNA target on MO capture probes could be reduced from ∼24 h to 30 min, with an increase in detection speed by 48 times. This ICP-enhanced hybridization method not only significantly decreases the detection time but also makes workflow simple to use, circumventing use of quantitative reverse transcription polymerase chain reaction or other conventional enzyme-based amplification methods that can cause artifacts.
Rapid detection of exosomal microRNA biomarkers by electrokinetic concentration for liquid biopsy on chip
Lucia S. Cheung, Xi Wei, Diogo Martins, Yong-Ak Song; Rapid detection of exosomal microRNA biomarkers by electrokinetic concentration for liquid biopsy on chip. Biomicrofluidics 1 January 2018; 12 (1): 014104. https://doi.org/10.1063/1.5009719
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