In this study, a low-cost, compact biochip is designed and fabricated for protein detection. Nanofractures formed by self-assembled gold nanoparticles at junction gaps are applied for ion enrichment and depletion to create a trapping zone when electroosmotic flow occurs in microchannels. An impedance measurement module is implemented based on the lock-in amplifier technique to measure the impedance change during antibody growth on the gold electrodes which is caused by trapped proteins in the detection region. The impedance measurement results confirm the presence of trapped proteins. Distinguishable impedance profiles, measured at frequencies in the range of 10–100 kHz, for the detection area taken before and after the presence of proteins validate the performance of the proposed system.
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September 2017
Research Article|
October 23 2017
A compact microfluidic chip with integrated impedance biosensor for protein preconcentration and detection
Tuan Vu Quoc
;
Tuan Vu Quoc
1
Institute of Applied Physics and Scientific Instrument, Vietnam Academy of Science and Technology
, Hanoi, Vietnam
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Meng-Syuan Wu;
Meng-Syuan Wu
2
Department of Mechanical Engineering, National Chung Cheng University
, Chia-Yi, Taiwan
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Tung Thanh Bui
;
Tung Thanh Bui
3
University of Engineering and Technology, Vietnam National University
, Hanoi, Vietnam
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Trinh Chu Duc;
Trinh Chu Duc
a)
3
University of Engineering and Technology, Vietnam National University
, Hanoi, Vietnam
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Chun-Ping Jen
Chun-Ping Jen
a)
2
Department of Mechanical Engineering, National Chung Cheng University
, Chia-Yi, Taiwan
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a)
Authors to whom correspondence should be addressed: imecpj@ccu.edu.tw and trinhcd@vnu.edu.vn
Biomicrofluidics 11, 054113 (2017)
Article history
Received:
July 14 2017
Accepted:
October 06 2017
Citation
Tuan Vu Quoc, Meng-Syuan Wu, Tung Thanh Bui, Trinh Chu Duc, Chun-Ping Jen; A compact microfluidic chip with integrated impedance biosensor for protein preconcentration and detection. Biomicrofluidics 1 September 2017; 11 (5): 054113. https://doi.org/10.1063/1.4996118
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