An integrated microfluidic system capable of automatically identifying aptamers specific to cholangiocarcinoma (CCA) cells was developed herein. The developed system was capable of performing cell-based systematic evolution of ligands via an exponential enrichment (Cell-SELEX) process on-chip, and only six rounds of Cell-SELEX were required to identify high specificity aptamers; this represents a significant improvement in speed over conventional SELEX, in which 15–20 rounds are typically required. Using the microfluidic chip developed, three aptamers specific to CCA cells (one for SNU-478 cells and two for HuCCT-1 cells) were successfully screened. This automated system could be modified to uncover aptamer probes against other cancer cells, thereby allowing for earlier diagnosis and consequently a potentially improved prognosis.
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July 2017
Research Article|
July 05 2017
Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
Priya Gopinathan;
Priya Gopinathan
1
Institute of Nanoengineering and Microsystems, National Tsing Hua University
, Hsinchu, Taiwan
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Lien-Yu Hung;
Lien-Yu Hung
2
Department of Power Mechanical Engineering, National Tsing Hua University
, Hsinchu 30013, Taiwan
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Chih-Hung Wang;
Chih-Hung Wang
2
Department of Power Mechanical Engineering, National Tsing Hua University
, Hsinchu 30013, Taiwan
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Nai-Jung Chiang;
Nai-Jung Chiang
3
National Institute of Cancer Research, National Health Research Institutes
, Tainan, Taiwan
4
Division of Hematology and Oncology, Department of Internal Medicine, National Cheng Kung University Hospital
, Tainan, Taiwan
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Yu-Chun Wang;
Yu-Chun Wang
5
Institute of Clinical Medicine, National Cheng Kung University Hospital
, Tainan, Taiwan
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Yan-Shen Shan;
Yan-Shen Shan
a)
5
Institute of Clinical Medicine, National Cheng Kung University Hospital
, Tainan, Taiwan
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Gwo-Bin Lee
Gwo-Bin Lee
a)
1
Institute of Nanoengineering and Microsystems, National Tsing Hua University
, Hsinchu, Taiwan
2
Department of Power Mechanical Engineering, National Tsing Hua University
, Hsinchu 30013, Taiwan
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a)
Authors to whom correspondence should be addressed: gwobin@pme.nthu.edu.tw, Tel.: +886-3-5715131 ext. 33765, Fax: +886-3-5742495 and ysshan@mail.ncku.edu.tw, Tel.: +886-6-2353535 ext. 3105, Fax: +886-6-2758781.
Note: The preliminary results of this study have been presented at the MicroTAS 2016, Dublin, 2016.
Biomicrofluidics 11, 044101 (2017)
Article history
Received:
April 05 2017
Accepted:
June 19 2017
Citation
Priya Gopinathan, Lien-Yu Hung, Chih-Hung Wang, Nai-Jung Chiang, Yu-Chun Wang, Yan-Shen Shan, Gwo-Bin Lee; Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform. Biomicrofluidics 1 July 2017; 11 (4): 044101. https://doi.org/10.1063/1.4991005
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