Cell disruption plays a vital role in detection of intracellular components which contain information about genetic and disease characteristics. In this paper, we demonstrate a novel microfluidic platform based on an on-chip micropump for mechanical cell disruption and sample transport. A 50 μl cell sample can be effectively lysed through on-chip multi-disruption in 36 s without introducing any chemical agent and suffering from clogging by cellular debris. After 30 cycles of circulating disruption, 80.6% and 90.5% cell disruption rates were achieved for the HEK293 cell sample and human natural killer cell sample, respectively. Profiting from the feature of pump-on-chip, the highly integrated platform enables more convenient and cost-effective cell disruption for the analysis of intracellular components.
A mechanical cell disruption microfluidic platform based on an on-chip micropump
Yinuo Cheng, Yue Wang, Zhiyuan Wang, Liang Huang, Mingzhao Bi, Wenxiao Xu, Wenhui Wang, Xiongying Ye; A mechanical cell disruption microfluidic platform based on an on-chip micropump. Biomicrofluidics 1 March 2017; 11 (2): 024112. https://doi.org/10.1063/1.4979100
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