The study of inflammatory bowel disease, including Ulcerative Colitis and Crohn's Disease, has relied largely upon the use of animal or cell culture models; neither of which can represent all aspects of the human pathophysiology. Presented herein is a dual flow microfluidic device which holds full thickness human intestinal tissue in a known orientation. The luminal and serosal sides are independently perfused ex vivo with nutrients with simultaneous waste removal for up to 72 h. The microfluidic device maintains the viability and integrity of the tissue as demonstrated through Haematoxylin & Eosin staining, immunohistochemistry and release of lactate dehydrogenase. In addition, the inflammatory state remains in the tissue after perfusion on the device as determined by measuring calprotectin levels. It is anticipated that this human model will be extremely useful for studying the biology and testing novel interventions in diseased tissue.
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November 2016
Research Article|
November 01 2016
A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow
A. Dawson;
A. Dawson
a)
1Faculty of Life Sciences,
University of Hull
, Cottingham Road, Hull HU6 7RX, United Kingdom
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C. Dyer;
C. Dyer
1Faculty of Life Sciences,
University of Hull
, Cottingham Road, Hull HU6 7RX, United Kingdom
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J. Macfie;
J. Macfie
2
Scarborough Hospital
, Woodlands Drive, Scarborough Y012 6QL, United Kingdom
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J. Davies;
J. Davies
3General Surgery,
Castlehill Hospital
, Castle Rd, Cottingham HU16 5JQ, United Kingdom
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L. Karsai;
L. Karsai
4Pathology Building,
Hull Royal Infirmary
, Anlaby Road, Hull HU3 2JZ, United Kingdom
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J. Greenman;
J. Greenman
1Faculty of Life Sciences,
University of Hull
, Cottingham Road, Hull HU6 7RX, United Kingdom
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M. Jacobsen
M. Jacobsen
5Department of Medicine,
Ostfold Hospital Trust
, Fredrikstad, Norway
6Institute for Clinical Research,
University of Oslo
, Oslo Norway
7
Institute for Chemistry and Biology
, NMBU, Ås, Norway
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A. Dawson
1,a)
C. Dyer
1
J. Macfie
2
J. Davies
3
L. Karsai
4
J. Greenman
1
M. Jacobsen
5,6,7
1Faculty of Life Sciences,
University of Hull
, Cottingham Road, Hull HU6 7RX, United Kingdom
2
Scarborough Hospital
, Woodlands Drive, Scarborough Y012 6QL, United Kingdom
3General Surgery,
Castlehill Hospital
, Castle Rd, Cottingham HU16 5JQ, United Kingdom
4Pathology Building,
Hull Royal Infirmary
, Anlaby Road, Hull HU3 2JZ, United Kingdom
5Department of Medicine,
Ostfold Hospital Trust
, Fredrikstad, Norway
6Institute for Clinical Research,
University of Oslo
, Oslo Norway
7
Institute for Chemistry and Biology
, NMBU, Ås, Norway
a)
A. Dawson awarded National Scholar Award for Norway, Poster presentation, UEG Conference, Vienna, October 2014.
Biomicrofluidics 10, 064101 (2016)
Article history
Received:
August 10 2016
Accepted:
September 30 2016
Connected Content
Citation
A. Dawson, C. Dyer, J. Macfie, J. Davies, L. Karsai, J. Greenman, M. Jacobsen; A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow. Biomicrofluidics 1 November 2016; 10 (6): 064101. https://doi.org/10.1063/1.4964813
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