Surface acoustic wave (SAW), a nanometer amplitude electroelastic wave generated and propagated on low-loss piezoelectric substrates (such as LiNbO3), is an extremely efficient solid–fluid energy transfer mechanism. The present study explores the use of SAW nebulization as a solution for effective pulmonary peptide delivery. In vitro deposition characteristics of the nebulized peptides were determined using a Next Generation Cascade Impactor. 70% of the peptide-laden aerosols generated were within a size distribution favorable for deep lung distribution. The integrity of the nebulized peptides was found to be retained, as shown via mass spectrometry. The anti-mycobacterial activity of the nebulized peptides was found to be uncompromised compared with their non-nebulized counterparts, as demonstrated by the minimum inhibition concentration and the colony forming inhibition activity. The peptide concentration and volume recoveries for the SAW nebulizer were significantly higher than 90% and found to be insensitive to variation in the peptide sequences. These results demonstrate the potential of the SAW nebulization platform as an effective delivery system of therapeutic peptides through the respiratory tract to the deep lung.
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Stability and efficacy of synthetic cationic antimicrobial peptides nebulized using high frequency acoustic waves
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Research Article|
June 07 2016
Stability and efficacy of synthetic cationic antimicrobial peptides nebulized using high frequency acoustic waves
Ying Wang;
Ying Wang
1Department of Pharmacy,
National University of Singapore
, 18 Science Drive 4, Singapore, Singapore
117543
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Amgad R. Rezk;
Amgad R. Rezk
2Micro/Nanophysics Research Laboratory, School of Civil, Environmental and Chemical Engineering,
RMIT University
, Melbourne, Victoria 3000, Australia
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Jasmeet Singh Khara;
Jasmeet Singh Khara
1Department of Pharmacy,
National University of Singapore
, 18 Science Drive 4, Singapore, Singapore
117543
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Leslie Y. Yeo
;
Leslie Y. Yeo
a)
2Micro/Nanophysics Research Laboratory, School of Civil, Environmental and Chemical Engineering,
RMIT University
, Melbourne, Victoria 3000, Australia
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Pui Lai Rachel Ee
Pui Lai Rachel Ee
a)
1Department of Pharmacy,
National University of Singapore
, 18 Science Drive 4, Singapore, Singapore
117543
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a)
Authors to whom correspondence should be addressed. Electronic addresses: [email protected] and [email protected].
Biomicrofluidics 10, 034115 (2016)
Article history
Received:
April 26 2016
Accepted:
May 26 2016
Citation
Ying Wang, Amgad R. Rezk, Jasmeet Singh Khara, Leslie Y. Yeo, Pui Lai Rachel Ee; Stability and efficacy of synthetic cationic antimicrobial peptides nebulized using high frequency acoustic waves. Biomicrofluidics 1 May 2016; 10 (3): 034115. https://doi.org/10.1063/1.4953548
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