Precise analysis of the aquatic cells and their responses to the toxic chemicals, i.e., water disinfective agents, is of crucial importance due to their role in the ecosystem. We demonstrate the application of the droplets based millifluidic tool for isolating and longtime monitoring of single Paramecium tetraurelia cells using a large number of water-in-oil emulsion droplets. Due to the automated monitoring of the fluorescence signal, the droplets containing cells are distinguished from the empty reservoirs. A viability indicator is used to follow the metabolic dynamic of the cells in every single droplet. Finally, we perform ecotoxicity tests in droplets, exposing the encapsulated paramecia cells to silver nitrate for determination of EC50 levels, and compare the output with the conventional microtiter plate assay.
Skip Nav Destination
Article navigation
March 2016
Research Article|
March 24 2016
Ecotoxicity assessment using ciliate cells in millifluidic droplets
Rico Illing;
Rico Illing
1Max Bergmann Center of Biomaterials and Institute for Materials Science,
Dresden University of Technology
, Budapesterstrasse 27, 01069 Dresden, Germany
2
Center for Advancing Electronics Dresden
, 01062 Dresden, Germany
Search for other works by this author on:
Corinna Burkart;
Corinna Burkart
3
Institute of Hydrobiology
, TU Dresden, 01062 Dresden, Germany
Search for other works by this author on:
Daniel Pfitzner;
Daniel Pfitzner
3
Institute of Hydrobiology
, TU Dresden, 01062 Dresden, Germany
Search for other works by this author on:
Dirk Jungmann;
Dirk Jungmann
3
Institute of Hydrobiology
, TU Dresden, 01062 Dresden, Germany
Search for other works by this author on:
Larysa Baraban;
Larysa Baraban
a)
1Max Bergmann Center of Biomaterials and Institute for Materials Science,
Dresden University of Technology
, Budapesterstrasse 27, 01069 Dresden, Germany
Search for other works by this author on:
Gianaurelio Cuniberti
Gianaurelio Cuniberti
1Max Bergmann Center of Biomaterials and Institute for Materials Science,
Dresden University of Technology
, Budapesterstrasse 27, 01069 Dresden, Germany
2
Center for Advancing Electronics Dresden
, 01062 Dresden, Germany
Search for other works by this author on:
a)
Author to whom correspondence should be addressed. Electronic mail: [email protected].
Biomicrofluidics 10, 024115 (2016)
Article history
Received:
January 10 2016
Accepted:
March 15 2016
Citation
Rico Illing, Corinna Burkart, Daniel Pfitzner, Dirk Jungmann, Larysa Baraban, Gianaurelio Cuniberti; Ecotoxicity assessment using ciliate cells in millifluidic droplets. Biomicrofluidics 1 March 2016; 10 (2): 024115. https://doi.org/10.1063/1.4944869
Download citation file:
Pay-Per-View Access
$40.00
Sign In
You could not be signed in. Please check your credentials and make sure you have an active account and try again.
Citing articles via
Processing and inspection of high-pressure microfluidics systems: A review
Jiangyi Song, Shaoxin Meng, et al.
Design of 3D printed chip to improve sensitivity of platelet adhesion through reinjection: Effect of alcohol consumption on platelet adhesion
Haebeen Kim, Hae-Ryoun Park, et al.
Impact of dcEF on microRNA profiles in glioblastoma and exosomes using a novel microfluidic bioreactor
Hsieh-Fu Tsai, Amy Q. Shen
Related Content
Pb2+ Modulates Ca2+ Membrane Permeability In Paramecium
AIP Conference Proceedings (September 2004)
L‐glutamate Receptor In Paramecium
AIP Conference Proceedings (September 2004)
Development of a multitarget tracking system for paramecia
Rev. Sci. Instrum. (July 2010)
Reversible single cell trapping of Paramecium caudatum to correlate swimming behavior and membrane state
Biomicrofluidics (March 2022)
On random search: Collection kinetics of Paramecia into a trap embedded in a closed domain
Am. J. Phys. (June 2010)