We aimed to characterize aging of single vascular endothelial cells, which are indicators of senescence, using micro electrical impedance spectroscopy (μEIS) for the first time. The proposed μEIS was equipped with two barriers under the membrane actuator near the sensing electrodes, increasing its cell-trapping capability and minimizing the interference between the target cell and subsequent cells. The cell-trapping capability in μEIS with barriers was considerably improved (90%) with a capture time of 5 s or less, compared to μEIS without barriers (30%). Cells were extracted from transgenic zebrafish to minimize an initial discrepancy originating from genetic differences. In order to estimate useful parameters, cytoplasm resistance and membrane capacitance were estimated by fitting an electrical equivalent circuit to the data of ex vivo sensor output. The estimated cytoplasm resistance and membrane capacitance in the younger vascular endothelial cells were 20.16 ± 0.79 kΩ and 17.46 ± 0.76 pF, respectively, whereas those in the older cells were 17.81 ± 0.98 kΩ and 20.08 ± 1.38 pF, respectively. Discrimination of each group with different aging showed statistical significance in terms of cytoplasm resistance (p < 0.001) and membrane capacitance (p < 0.001). Considering both of the sensor and cellular level, the optimal frequency was determined as 1 MHz at which the electrical impedance of each group was clearly discriminated (p < 0.001).
Skip Nav Destination
,
,
,
,
,
,
,
,
Article navigation
January 2016
Research Article|
January 28 2016
Ex vivo characterization of age-associated impedance changes of single vascular endothelial cells using micro electrical impedance spectroscopy with a cell trap Available to Purchase
Yangkyu Park;
Yangkyu Park
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
Search for other works by this author on:
Jung-Joon Cha
;
Jung-Joon Cha
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
Search for other works by this author on:
Seungwan Seo;
Seungwan Seo
2Department of Mechatronics,
GIST
, Gwangju 500-712, South Korea
Search for other works by this author on:
Joho Yun;
Joho Yun
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
Search for other works by this author on:
Hyeon Woo Kim;
Hyeon Woo Kim
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
Search for other works by this author on:
Changju Park;
Changju Park
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
Search for other works by this author on:
Giseok Gang;
Giseok Gang
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
Search for other works by this author on:
Juhun Lim;
Juhun Lim
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
Search for other works by this author on:
Jong-Hyun Lee
Jong-Hyun Lee
a)
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
2Department of Mechatronics,
GIST
, Gwangju 500-712, South Korea
Search for other works by this author on:
Yangkyu Park
1
Jung-Joon Cha
1
Seungwan Seo
2
Joho Yun
1
Hyeon Woo Kim
1
Changju Park
1
Giseok Gang
1
Juhun Lim
1
Jong-Hyun Lee
1,2,a)
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
2Department of Mechatronics,
GIST
, Gwangju 500-712, South Korea
a)
Author to whom correspondence should be addressed. Electronic mail: [email protected]. Tel.: 8262 715 2395. Fax: 8262 715 2384.
Biomicrofluidics 10, 014114 (2016)
Article history
Received:
January 13 2016
Accepted:
January 19 2016
Citation
Yangkyu Park, Jung-Joon Cha, Seungwan Seo, Joho Yun, Hyeon Woo Kim, Changju Park, Giseok Gang, Juhun Lim, Jong-Hyun Lee; Ex vivo characterization of age-associated impedance changes of single vascular endothelial cells using micro electrical impedance spectroscopy with a cell trap. Biomicrofluidics 1 January 2016; 10 (1): 014114. https://doi.org/10.1063/1.4941044
Download citation file:
Pay-Per-View Access
$40.00
Sign In
You could not be signed in. Please check your credentials and make sure you have an active account and try again.
Citing articles via
A tracking algorithm for finite-size particles
Aryan Mehboudi, Shrawan Singhal, et al.
Investigation of pressure balance in proximity of sidewalls in deterministic lateral displacement
Aryan Mehboudi, Shrawan Singhal, et al.
Recent advances in microscale techniques for red blood cells manipulation
Huihui Xu, Huijing Zhang, et al.
Related Content
Micro electrical impedance spectroscopy on a needle for ex vivo discrimination between human normal and cancer renal tissues
Biomicrofluidics (May 2016)
Discrimination between the human prostate normal cell and cancer cell by using a novel electrical impedance spectroscopy controlling the cross-sectional area of a microfluidic channel
Biomicrofluidics (August 2013)
Microfluidic impedance cytometry of tumour cells in blood
Biomicrofluidics (December 2014)
Single Crystal Diamond Beam Position Monitors with Radiofrequency Electronic Readout
AIP Conf. Proc. (June 2010)
Top-up operation at Pohang Light Source-II
Rev. Sci. Instrum. (May 2014)