We aimed to characterize aging of single vascular endothelial cells, which are indicators of senescence, using micro electrical impedance spectroscopy (μEIS) for the first time. The proposed μEIS was equipped with two barriers under the membrane actuator near the sensing electrodes, increasing its cell-trapping capability and minimizing the interference between the target cell and subsequent cells. The cell-trapping capability in μEIS with barriers was considerably improved (90%) with a capture time of 5 s or less, compared to μEIS without barriers (30%). Cells were extracted from transgenic zebrafish to minimize an initial discrepancy originating from genetic differences. In order to estimate useful parameters, cytoplasm resistance and membrane capacitance were estimated by fitting an electrical equivalent circuit to the data of ex vivo sensor output. The estimated cytoplasm resistance and membrane capacitance in the younger vascular endothelial cells were 20.16 ± 0.79 kΩ and 17.46 ± 0.76 pF, respectively, whereas those in the older cells were 17.81 ± 0.98 kΩ and 20.08 ± 1.38 pF, respectively. Discrimination of each group with different aging showed statistical significance in terms of cytoplasm resistance (p < 0.001) and membrane capacitance (p < 0.001). Considering both of the sensor and cellular level, the optimal frequency was determined as 1 MHz at which the electrical impedance of each group was clearly discriminated (p < 0.001).
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January 2016
Research Article|
January 28 2016
Ex vivo characterization of age-associated impedance changes of single vascular endothelial cells using micro electrical impedance spectroscopy with a cell trap
Yangkyu Park;
Yangkyu Park
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
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Jung-Joon Cha
;
Jung-Joon Cha
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
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Seungwan Seo;
Seungwan Seo
2Department of Mechatronics,
GIST
, Gwangju 500-712, South Korea
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Joho Yun;
Joho Yun
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
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Hyeon Woo Kim;
Hyeon Woo Kim
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
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Changju Park;
Changju Park
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
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Giseok Gang;
Giseok Gang
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
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Juhun Lim;
Juhun Lim
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
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Jong-Hyun Lee
Jong-Hyun Lee
a)
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
2Department of Mechatronics,
GIST
, Gwangju 500-712, South Korea
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Yangkyu Park
1
Jung-Joon Cha
1
Seungwan Seo
2
Joho Yun
1
Hyeon Woo Kim
1
Changju Park
1
Giseok Gang
1
Juhun Lim
1
Jong-Hyun Lee
1,2,a)
1Department of Medical System Engineering,
Gwangju Institute of Science and Technology (GIST)
, Gwangju 500-712, South Korea
2Department of Mechatronics,
GIST
, Gwangju 500-712, South Korea
a)
Author to whom correspondence should be addressed. Electronic mail: [email protected]. Tel.: 8262 715 2395. Fax: 8262 715 2384.
Biomicrofluidics 10, 014114 (2016)
Article history
Received:
January 13 2016
Accepted:
January 19 2016
Citation
Yangkyu Park, Jung-Joon Cha, Seungwan Seo, Joho Yun, Hyeon Woo Kim, Changju Park, Giseok Gang, Juhun Lim, Jong-Hyun Lee; Ex vivo characterization of age-associated impedance changes of single vascular endothelial cells using micro electrical impedance spectroscopy with a cell trap. Biomicrofluidics 1 January 2016; 10 (1): 014114. https://doi.org/10.1063/1.4941044
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