We present a dielectric model and its parameters for Chinese hamster ovary (CHO) cells based on a double-shell structure which includes the cell membrane, cytoplasm, nuclear envelope, and nucleoplasm. Employing a dielectrophoresis (DEP) based technique and a microfluidic system, the DEP response of many single CHO cells is measured and the spectrum of the Clausius-Mossotti factor is obtained. The dielectric parameters of the model are then extracted by curve-fitting to the measured spectral data. Using this approach over the 0.6–10 MHz frequency range, we report the values for CHO cells' membrane permittivity, membrane thickness, cytoplasm conductivity, nuclear envelope permittivity, and nucleoplasm conductivity. The size of the cell and its nuclei are obtained using optical techniques.
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January 2016
Research Article|
January 21 2016
Dielectric model for Chinese hamster ovary cells obtained by dielectrophoresis cytometry
E. Salimi;
E. Salimi
1Department of Electrical and Computer Engineering,
University of Manitoba
, Winnipeg, Manitoba R3T 5V6, Canada
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K. Braasch;
K. Braasch
2Department of Microbiology,
University of Manitoba
, Winnipeg, Manitoba R3T 2N2, Canada
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M. Butler;
M. Butler
2Department of Microbiology,
University of Manitoba
, Winnipeg, Manitoba R3T 2N2, Canada
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D. J. Thomson;
D. J. Thomson
1Department of Electrical and Computer Engineering,
University of Manitoba
, Winnipeg, Manitoba R3T 5V6, Canada
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G. E. Bridges
G. E. Bridges
a)
1Department of Electrical and Computer Engineering,
University of Manitoba
, Winnipeg, Manitoba R3T 5V6, Canada
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a)
Author to whom correspondence should be addressed. Electronic mail: [email protected]
Biomicrofluidics 10, 014111 (2016)
Article history
Received:
December 04 2015
Accepted:
January 11 2016
Citation
E. Salimi, K. Braasch, M. Butler, D. J. Thomson, G. E. Bridges; Dielectric model for Chinese hamster ovary cells obtained by dielectrophoresis cytometry. Biomicrofluidics 1 January 2016; 10 (1): 014111. https://doi.org/10.1063/1.4940432
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