The fabrication of biomimetic scaffolding is a challenging issue in tissue engineering. Scaffolds must be designed with micrometer precision to enable cell proliferation and tissue growth, requiring customization based on the type of tissue being developed. Biomimetic scaffolds have attracted interest for their potential in spinal fusion applications. By providing a structured environment to promote osteogenesis, these materials offer a robust and minimally invasive means to fuse vertebrae. The present study describes the successful preparation of a biomimetic collagen/hydroxyapatite hierarchical scaffold, with each strut having several microchannels via 3D printing, leaching, and coating processes (i.e., one-way shape morphing, 4D printing). The biophysical properties of the scaffold were analyzed, as were its various cellular activities, using human adipose stem cells. This biomimetic microchannel scaffold demonstrated great potential for osteogenic activities in vitro and significantly increased new bone formation and ingrowth of blood vessels in vivo in a mouse model of posterolateral lumbar spinal fusion. These in vitro and in vivo results suggest that the microchannel collagen/hydroxyapatite scaffold could act as a potential bone graft substitute to promote high rates of successful fusion.
Skip Nav Destination
Bone tissue engineering via application of a collagen/hydroxyapatite 4D-printed biomimetic scaffold for spinal fusion
,
,
,
,
,
,
,
,
Article navigation
June 2021
Research Article|
May 04 2021
Bone tissue engineering via application of a collagen/hydroxyapatite 4D-printed biomimetic scaffold for spinal fusion
Hanjun Hwangbo;
Hanjun Hwangbo
1
Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University
, Suwon 16419, South Korea
Search for other works by this author on:
Hyeongjin Lee;
Hyeongjin Lee
1
Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University
, Suwon 16419, South Korea
Search for other works by this author on:
Eun Ji Roh;
Eun Ji Roh
2
Department of Neurosurgery, CHA University School of Medicine, CHA Bundang Medical Center
, Seongnam-si, South Korea
Search for other works by this author on:
WonJin Kim
;
WonJin Kim
1
Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University
, Suwon 16419, South Korea
Search for other works by this author on:
Hari Prasad Joshi;
Hari Prasad Joshi
2
Department of Neurosurgery, CHA University School of Medicine, CHA Bundang Medical Center
, Seongnam-si, South Korea
Search for other works by this author on:
Su Yeon Kwon;
Su Yeon Kwon
2
Department of Neurosurgery, CHA University School of Medicine, CHA Bundang Medical Center
, Seongnam-si, South Korea
Search for other works by this author on:
Un Yong Choi;
Un Yong Choi
2
Department of Neurosurgery, CHA University School of Medicine, CHA Bundang Medical Center
, Seongnam-si, South Korea
Search for other works by this author on:
In-Bo Han
;
In-Bo Han
a)
2
Department of Neurosurgery, CHA University School of Medicine, CHA Bundang Medical Center
, Seongnam-si, South Korea
Search for other works by this author on:
Geun Hyung Kim
Geun Hyung Kim
a)
1
Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University
, Suwon 16419, South Korea
3
Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University
, Suwon 16419, South Korea
Search for other works by this author on:
Hanjun Hwangbo
1
Hyeongjin Lee
1
Eun Ji Roh
2
WonJin Kim
1
Hari Prasad Joshi
2
Su Yeon Kwon
2
Un Yong Choi
2
In-Bo Han
2,a)
Geun Hyung Kim
1,3,a)
1
Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University
, Suwon 16419, South Korea
2
Department of Neurosurgery, CHA University School of Medicine, CHA Bundang Medical Center
, Seongnam-si, South Korea
3
Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University
, Suwon 16419, South Korea
Appl. Phys. Rev. 8, 021403 (2021)
Article history
Received:
October 29 2020
Accepted:
March 22 2021
Citation
Hanjun Hwangbo, Hyeongjin Lee, Eun Ji Roh, WonJin Kim, Hari Prasad Joshi, Su Yeon Kwon, Un Yong Choi, In-Bo Han, Geun Hyung Kim; Bone tissue engineering via application of a collagen/hydroxyapatite 4D-printed biomimetic scaffold for spinal fusion. Appl. Phys. Rev. 1 June 2021; 8 (2): 021403. https://doi.org/10.1063/5.0035601
Download citation file:
Pay-Per-View Access
$40.00
Sign In
You could not be signed in. Please check your credentials and make sure you have an active account and try again.
Citing articles via
Architected acoustic metamaterials: An integrated design perspective
G. Comandini, M. Ouisse, et al.
Smart biomaterials in healthcare: Breakthroughs in tissue engineering, immunomodulation, patient-specific therapies, and biosensor applications
Ansheed Raheem, Kalpana Mandal, et al.
Roadmap for focused ion beam technologies
Katja Höflich, Gerhard Hobler, et al.
Related Content
Neuralized and vascularized fast bone regeneration using recombinant humanized type 1 collagen and native bone composite inorganic salts
APL Mater. (March 2025)
New products tissue-engineering in the treatment of spinal cord injury
AIP Conf. Proc. (November 2015)
Development of collagen-based bioinks for cartilage tissue engineering applications
AIP Conf. Proc. (August 2024)
Emergence of functional neuromuscular junctions in an engineered, multicellular spinal cord-muscle bioactuator
APL Bioeng. (April 2020)
Biohybrid robot with skeletal muscle tissue covered with a collagen structure for moving in air
APL Bioeng. (April 2020)