This letter reported the biophysical characterization of immunophenotypically distinct hematopoietic cells from normal and leukemic sources, through manipulation with optical tweezers at single cell level. The results show that the percentage of cells that are stretchable and their deformability are significantly higher in the more primitive cell populations. This study provides the evidence that normal and leukemic hematopoietic cell populations with distinct primitiveness exhibit differential biophysical properties. These findings raise a hypothesis that the high deformability may be related to the unique functions and activities of primitive hematopoietic cells.
REFERENCES
1.
G.
Bao
and S.
Suresh
, Nat. Mater.
2
, 715
(2003
).2.
A. R.
Bausch
and K.
Kroy
, Nat. Phys.
2
, 231
(2006
).3.
M.
Nagayama
, H.
Haga
, and K.
Kawabata
, Cell Motil. Cytoskeleton
50
, 173
(2001
).4.
F.
Chowdhury
, S.
Na
, D.
Li
, Y.-C.
Poh
, T. S.
Tanaka
, F.
Wang
, and N.
Wang
, Nat. Mater.
9
, 82
(2010
).5.
G.
Yourek
, M. A.
Hussain
, and J. J.
Mao
, ASAIO J.
53
, 219
(2007
).6.
G.
Ofek
, V. P.
Willard
, E. J.
Koay
, J. C.
Hu
, P.
Lin
, and K. A.
Athanasiou
, ASME J. Biomech. Eng.
131
, 061011
(2009
).7.
S. E.
Cross
, Y.-S.
Jin
, J.
Rao
, and J. K.
Gimzewski
, Nat. Nanotechnol.
2
, 780
(2007
).8.
S.
Suresh
, J.
Spatz
, J. P.
Mills
, A.
Micoulet
, D.
Mao
, C. T.
Lim
, M.
Beil
, and T.
Seufferlein
, Acta Biomater.
1
, 15
(2005
).9.
D.
Bonnet
and J. E.
Dick
, Nat. Med.
3
, 730
(1997
).10.
A.
Cheung
, Chow
, R.
Liang
, and A.
Leung
, Br. J. Haematol.
144
, 484
(2008
).11.
A.
Ashkin
, Phys. Rev. Lett.
24
, 156
(1970
).12.
Y.
Wu
, D.
Sun
, and W.
Huang
, J. Biomech.
44
, 741
(2010
).13.
S.
Henon
, G.
Lenormand
, A.
Richert
, and F.
Gallet
, Biophys. J.
76
, 1145
(1999
).14.
Y.
Tan
, D.
Sun
, J.
Wang
, and W.
Huang
, IEEE Trans. Biomed. Eng.
57
, 1816
(2010
).15.
M.
Khan
, H.
Soni
, and A. K.
Sood
, Appl. Phys. Lett.
95
, 233703
(2009
).16.
See supplementary material at http://dx.doi.org/10.1063/1.3610938 for a more detailed description of cell collection, elasticity measurement with optical tweezers, statistical analysis, and other data.
17.
Y.
Tan
, D.
Sun
, and W.
Huang
, ASME J. Biomech. Eng.
132
, 044504
(2010
).18.
Y.
Tan
, A. Y. H.
Leung
, K.
Wang
, T.-K.
Fung
, and D.
Sun
, IEEE Nanotechnol. Mag.
5
, 17
(2011
).19.
M. J.
Wozniak
, N.
Kawazoe
, T.
Tateishi
, and G.
Chen
, Soft Matter
6
, 2462
(2010
).20.
A.
Wilson
and A.
Trumpp
, Nat. Rev. Immun.
6
, 93
(2006
).© 2011 American Institute of Physics.
2011
American Institute of Physics
You do not currently have access to this content.