Electroporation is the formation of reversible hydrophilic pores in the cell membrane under electric fields. Severity of electroporation is challenging to measure and quantify. An image analysis method is developed, and the initial results with a fabricated microfluidic device are reported. The microfluidic device contains integrated microchannels and coplanar interdigitated electrodes allowing low-voltage operation and low-power consumption. Noninvasive human buccal cell samples were specifically stained, and electroporation was induced. Captured image sequences were analyzed for pixel color ranges to quantify the severity of electroporation. The method can detect even a minor occurrence of electroporation and can perform comparative studies.
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4 April 2011
Research Article|
April 08 2011
Identifying severity of electroporation through quantitative image analysis
Bashir I. Morshed;
Bashir I. Morshed
a)
1Medical Devices Innovation Institute,
University of Ottawa
, Ontario K1Y4W7, Canada
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Maitham Shams;
Maitham Shams
2Department of Electronics,
Carleton University
, Ottawa, Ontario K1S5B6, Canada
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Tofy Mussivand
Tofy Mussivand
1Medical Devices Innovation Institute,
University of Ottawa
, Ontario K1Y4W7, Canada
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a)
Electronic mail: bashirmorshed@gmail.com.
Appl. Phys. Lett. 98, 143704 (2011)
Article history
Received:
February 09 2011
Accepted:
March 08 2011
Citation
Bashir I. Morshed, Maitham Shams, Tofy Mussivand; Identifying severity of electroporation through quantitative image analysis. Appl. Phys. Lett. 4 April 2011; 98 (14): 143704. https://doi.org/10.1063/1.3575561
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