The FluidFM technology uses microchanneled atomic force microscope cantilevers that are fixed to a drilled atomic force microscope cantilevers probeholder. A continuous fluidic circuit is thereby achieved extending from an external liquid reservoir, through the probeholder and the hollow cantilever to the tip aperture. In this way, both overpressure and an underpressure can be applied to the liquid reservoir and hence to the built-in fluidic circuit. We describe in this letter how standard atomic force microscopy in combination with regulated pressure differences inside the microchanneled cantilevers can be used to displace living organisms with micrometric precision in a nondestructive way. The protocol is applicable to both eukaryotic and prokaryotic cells (e.g., mammalian cells, yeasts, and bacteria) in physiological buffer. By means of this procedure, cells can also be transferred from one glass slide to another one or onto an agar medium.

1.
D.
Di Carlo
and
L. P.
Lee
,
Anal. Chem.
78
,
7918
(
2006
).
2.
A.
Ashkin
and
J. M.
Dziedzic
,
Science
235
,
1517
(
1987
).
3.
A.
Ashkin
,
J. M.
Dziedzic
, and
T.
Yamane
,
Nature (London)
330
,
769
(
1987
).
4.
A.
Ashkin
and
J. M.
Dziedzic
,
Proc. Natl. Acad. Sci. U.S.A.
86
,
7914
(
1989
).
5.
G.
Weber
and
K. O.
Greulich
,
Int. Rev. Cytol.
133
,
1
(
1992
).
6.
K.
Svoboda
and
S. M.
Block
,
Annu. Rev. Biophys. Biomol. Struct.
23
,
247
(
1994
).
7.
P.
Jordan
,
J.
Leach
,
M.
Padgett
,
P.
Blackburn
,
N.
Isaacs
,
M.
Goksor
,
D.
Hanstorp
,
A.
Wright
,
J.
Girkin
, and
J.
Cooper
,
Lab Chip
5
,
1224
(
2005
).
8.
A. L.
Weisenhorn
,
P. K.
Hansma
,
T. R.
Albrecht
, and
C. F.
Quate
,
Appl. Phys. Lett.
54
,
2651
(
1989
).
9.
W. H.
Wright
,
G. J.
Sonek
, and
M. W.
Berns
,
Appl. Phys. Lett.
63
,
715
(
1993
).
10.
K.
König
,
H.
Liang
,
M. W.
Berns
, and
B. J.
Tromberg
,
Opt. Lett.
21
,
1090
(
1996
).
11.
K. C.
Neuman
,
E. H.
Chadd
,
G. F.
Liou
,
K.
Bergman
, and
S. M.
Block
,
Biophys. J.
77
,
2856
(
1999
).
12.
M. B.
Rasmussen
,
L. B.
Oddershede
, and
H.
Siegumfeldtl
,
Appl. Environ. Microbiol.
74
,
2441
(
2008
).
13.
T. K.
Chowdhury
,
J. Phys. E
2
,
1087
(
1969
).
15.
D.
Sanchez
,
N.
Johnson
,
C.
Li
,
P.
Novak
,
J.
Rheinlaender
,
Y. J.
Zhang
,
U.
Anand
,
P.
Anand
,
J.
Gorelik
,
G. I.
Frolenkov
,
C.
Benham
,
M.
Lab
,
V. P.
Ostanin
,
T. E.
Schaffer
,
D.
Klenerman
, and
Y. E.
Korchev
,
Biophys. J.
95
,
3017
(
2008
).
16.
G.
Binnig
,
C. F.
Quate
, and
C.
Gerber
,
Phys. Rev. Lett.
56
,
930
(
1986
).
17.
P. H.
Tsang
,
G. L.
Li
,
Y. V.
Brun
,
L.
Ben Freund
, and
J. X.
Tang
,
Proc. Natl. Acad. Sci. U.S.A.
103
,
5764
(
2006
).
18.
M.
Benoit
,
D.
Gabriel
,
G.
Gerisch
, and
H. E.
Gaub
,
Nat. Cell Biol.
2
,
313
(
2000
).
19.
X. H.
Zhang
,
A.
Chen
,
D.
De Leon
,
H.
Li
,
E.
Noiri
,
V. T.
Moy
, and
M. S.
Goligorsky
,
Am. J. Physiol. Heart Circ. Physiol.
286
,
H359
(
2003
).
20.
V.
Vogel
and
M.
Sheetz
,
Nat. Rev. Mol. Cell Biol.
7
,
265
(
2006
).
21.
C.
Selhuber-Unkel
,
B. I. F. Futura
22
,
197
(
2007
).
22.
T.
Ludwig
,
R.
Kirmse
,
K.
Poole
, and
U. S.
Schwarz
,
Pfluegers Arch. Eur. J. Physiol.
456
,
29
(
2008
).
23.
C. M.
Franz
and
P. -H.
Puech
,
Cell Mol Bioeng
1
,
289
(
2008
).
24.
A.
Meister
,
M.
Gabi
,
P.
Behr
,
P.
Studer
,
J.
Vörös
,
P.
Niedermann
,
J.
Bitterli
,
J.
Polesel-Maris
,
M.
Liley
,
H.
Heinzelmann
, and
T.
Zambelli
,
Nano Lett.
9
,
2501
(
2009
).
25.
F.
Kühner
,
R. A.
Lugmaier
,
S.
Mihatsch
, and
H. E.
Gaub
,
Rev. Sci. Instrum.
78
,
075105
(
2007
).
26.
See supplementary material at http://dx.doi.org/10.1063/1.3462979 for the description of the custom FluidFM, the “before-after” images of the yeasts manipulation and the description of the ”double-tube” tips microfabrication.
27.
S.
Deladi
,
N. R.
Tas
,
J. W.
Berenschot
,
G. J. M.
Krijnen
,
M. J.
de Boer
,
J. H.
de Boer
,
M.
Peter
, and
M. C.
Elwenspoek
,
Appl. Phys. Lett.
85
,
5361
(
2004
).
28.
K. H.
Kim
,
N.
Moldovan
, and
H. D.
Espinosa
,
Small
1
,
632
(
2005
).
29.
T. S.
Hug
,
T.
Biss
,
N. F.
de Rooij
, and
Q.
Staufer
Dig. Tech. Pap. Transducers
,
2
,
1191
, (
2005
).
30.
A.
Meister
,
J.
Przybylska
,
P.
Niedermann
,
C.
Santschi
, and
H.
Heinzelmann
,
NSTI-Nanotech 2008
(NSTI (Nano Science and Technology Institute, CRC, Taylor & Francis Group,
2008
), Vol.
3
, pp.
273
276
.
31.
N.
Kato
,
T.
Kawashima
,
T.
Shibata
,
T.
Mineta
, and
E.
Makino
,
Microelectronic Engineering
87
,
1185
(
2010
).
32.
S.
Deladi
,
J. W.
Berenschot
,
N. R.
Tas
,
G. J. M.
Krijnen
,
J. H.
de Boer
,
M. J.
de Boer
, and
M. C.
Elwenspoek
,
J. Micromech. Microeng.
15
,
528
(
2005
).
33.
N. P.
Huang
,
R.
Michel
,
J.
Vörös
,
M.
Textor
,
R.
Hofer
,
A.
Rossi
,
D. L.
Elbert
,
J. A.
Hubbell
, and
N. D.
Spencer
,
Langmuir
17
,
489
(
2001
).
34.
The stepper motor was used to detach cells because of the limited maximal extension of the piezo. As consequence, a sinusoidal noise was superimposed to the F(z) curves.
35.
C.
Selhuber-Unkel
,
T.
Erdmann
,
M.
Lopez-Garcia
,
H.
Kessler
,
U. S.
Schwarz
, and
J. P.
Spatz
,
Biophys. J.
98
,
543
(
2010
).
36.
G.
Weder
,
N.
Blondiaux
,
M.
Giazzon
,
N.
Matthey
,
M.
Klein
,
R.
Pugin
,
H.
Heinzelmann
, and
M.
Liley
,
Langmuir
26
,
8180
(
2010
).

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