In this study, we considered the interplay between the efficiency and cytotoxicity of multicomponent cationic liposome/DNA complexes, in cell lines of different origin, as NIH 3T3, HEK 293T, N18TG2, and SK-N-SH. We show that both efficiency and cytotoxicity vary considerably depending on used transfection agents and cells lines. Such variations are largely overcome when transfection is evaluated by a parameter, , that combines the percentages of transfected, nonviable, and nonadherent cells. These findings provide a strong validation of as an unbiased indicator for transfection performance across cell lines and transfection agents.
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© 2010 American Institute of Physics.
2010
American Institute of Physics
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