Heme proteins, such as hemoglobins and cytochromes, play important roles in various biological processes. Here we employ the two-photon excited photothermal effect as a contrast mechanism to map heme proteins distribution. Particularly, both a thermal lens scheme and a high-frequency modulation are utilized to enhance the signal-to-noise ratio. We demonstrate label-free imaging of individual red blood cells, subcellular distribution of cytochromes in live mammalian cells, and the microvascular networks in mouse ear tissue and in a zebrafish gill.
Label-free imaging of heme proteins with two-photon excited photothermal lens microscopy
Sijia Lu, Wei Min, Shasha Chong, Gary R. Holtom, X. Sunney Xie; Label-free imaging of heme proteins with two-photon excited photothermal lens microscopy. Appl. Phys. Lett. 15 March 2010; 96 (11): 113701. https://doi.org/10.1063/1.3308485
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