Biochemical modifications of single cells of human keratinocytes exposed to different concentrations of chlorpyriphos solutions were detected by means of Raman microspectroscopy. The modifications mainly consist of breakdown of amide linkage between aminoacids. Fragmentation of DNA bases and single aminoacids occurs as well. The protein and DNA damage are already detectable at a chlorpyriphos concentration value , which is at least three orders of magnitude lower than the concentration estimated as cytotoxic . Raman microspectroscopy technique results to be a very sensitive technique to detect molecular changes even in single human cells exposed to toxic pesticide.
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Raman spectra of cells exposed at chlorpyriphos concentration of and have not been reported because the former concentration is cytotoxic and about 72% are dead cells according to the viability test. Moreover, these cells detached from the substrate and Raman spectra cannot be measured. Further, the cellular morphology of remaining living cells (28%) resulted strong damage. At , about 56% of the cells were still alive but their Raman spectra were characterized by a large fluctuation from cell to cell as compared with the variability of the spectra exposed at lower chlorpyriphos concentration. Therefore, these Raman spectra are not reported because the standard deviation results are quite large with respect to that of the spectra of samples exposed to lower chlorpyriphos concentration and, then, the mean Raman signal measured over the investigated cells is not very meaningful.
© 2009 American Institute of Physics.
2009
American Institute of Physics
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